ENC1 Promotes the Malignant Progression and Metastasis by Suppressing TRIM21 Mediated Vimentin Degradation in Wilms Tumor DOI

Zuopeng Lu,

Hongjie Gao,

Fan Huang

et al.

Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Ectodermal neural cortex 1 (ENC1) is significantly upregulated in various cancers and shows a positive correlation with poor prognosis advanced clinical stages, such as colorectal cancer, endometrial cancer breast cancer. However, the role of ENC1 Wilms tumor (WT) has not been previously reported. In this study, we conducted several vitro functional experiments established xenograft models to confirm oncogenic potential ENC1. The binding proteins were identified through co-immunoprecipitation mass spectrometry screen mechanism malignant progression. Further analysis elucidated by which promotes tumorigenesis. results demonstrated that was overexpressed recurrence samples, elevated expression showing significant negative both overall survival recurrence-free patients. Functionally, oncogenicity assessment cell proliferation, migration, invasion capabilities. Mechanistically, immunoprecipitation spectrometry, Vimentin an interacting protein competed E3 ubiquitin ligase TRIM21 for binding, thereby reducing ubiquitination level enhancing its stability. conclusion, study demonstrates functions novel target disrupting TRIM21-mediated Vimentin, presents insights treatment development prognostic markers.

Language: Английский

Tumor Microenvironment-Derived Exosomes: A Double-Edged Sword for Advanced T Cell-Based Immunotherapy DOI
Ye Lu,

Jiarong Zheng,

Pei Lin

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

The tumor microenvironment (TME) plays a crucial role in cancer progression and immune evasion, partially mediated by the activity of TME-derived exosomes. These extracellular vesicles are pivotal shaping responses through transfer proteins, lipids, nucleic acids between cells, facilitating complex interplay that promotes growth metastasis. This review delves into dual roles exosomes TME, highlighting both their immunosuppressive functions emerging therapeutic potential. Exosomes can inhibit T cell function promote escape carrying immune-modulatory molecules, such as PD-L1, yet they also hold promise for therapy vehicles delivering antigens costimulatory signals. Additionally, discusses intricate crosstalk among various types within influencing to immunotherapies. Moreover, this highlights current challenges future directions. Collectively, elucidating detailed mechanisms which mediate offers promising avenue revolutionizing treatment. Understanding these interactions allows development targeted therapies manipulate exosomal pathways enhance system's response tumors.

Language: Английский

Citations

7
Post‐Translational Modifications of RNA‐Modifying Proteins in Cellular Dynamics and Disease Progression DOI Creative Commons
Yunfan Lin, Pei Lin, Ye Lu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(44)

Published: Oct. 8, 2024

Abstract RNA‐modifying proteins, classified as “writers,” “erasers,” and “readers,” dynamically modulate RNA by adding, removing, or interpreting chemical groups, thereby influencing stability, functionality, interactions. To date, over 170 distinct modifications more than 100 enzymes have been identified, with ongoing research expanding these numbers. Although significant progress has made in understanding modification, the regulatory mechanisms that govern proteins themselves remain insufficiently explored. Post‐translational (PTMs) such phosphorylation, ubiquitination, acetylation are crucial modulating function behavior of proteins. However, full extent PTM influence on their role disease development remains to be fully elucidated. This review addresses gaps offering a comprehensive analysis roles PTMs play regulating Mechanistic insights provided into how alter biological processes, contribute cellular function, drive progression. In addition, current landscape is examined, highlighting therapeutic potential targeting for precision medicine. By advancing networks, this seeks facilitate effective strategies inspire future critical area

Language: Английский

Citations

5
ENC1 Promotes the Malignant Progression and Metastasis by Suppressing TRIM21 Mediated Vimentin Degradation in Wilms Tumor DOI

Zuopeng Lu,

Hongjie Gao,

Fan Huang

et al.

Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Ectodermal neural cortex 1 (ENC1) is significantly upregulated in various cancers and shows a positive correlation with poor prognosis advanced clinical stages, such as colorectal cancer, endometrial cancer breast cancer. However, the role of ENC1 Wilms tumor (WT) has not been previously reported. In this study, we conducted several vitro functional experiments established xenograft models to confirm oncogenic potential ENC1. The binding proteins were identified through co-immunoprecipitation mass spectrometry screen mechanism malignant progression. Further analysis elucidated by which promotes tumorigenesis. results demonstrated that was overexpressed recurrence samples, elevated expression showing significant negative both overall survival recurrence-free patients. Functionally, oncogenicity assessment cell proliferation, migration, invasion capabilities. Mechanistically, immunoprecipitation spectrometry, Vimentin an interacting protein competed E3 ubiquitin ligase TRIM21 for binding, thereby reducing ubiquitination level enhancing its stability. conclusion, study demonstrates functions novel target disrupting TRIM21-mediated Vimentin, presents insights treatment development prognostic markers.

Language: Английский

Citations

0