Identification of ferroptosis-related gene signatures in temporal lobe epilepsy with hippocampal sclerosis DOI Creative Commons
Fan Gao, Jinzi Li

Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: April 2, 2025

Ferroptosis is a form of regulated cell death that damages neurons in the central nervous system. In this study, we aimed to construct ferroptosis-related gene signatures temporal lobe epilepsy with hippocampal sclerosis (TLE-HS) and explore their diagnostic role TLE-HS. The GSE205661 dataset was acquired for training purposes, while GSE71058 obtained serve as validation dataset. Subsequently, differentially expressed genes (FR-DEGs) TLE-HS were further analyzed. We used weighed co-expression network analysis (WGCNA) algorithm, single-factor logistic regression analysis, LASSO algorithm screen characteristic FR-DEGs. Then, receiver operating (ROC) evaluate value these disease diagnosis. Finally, long non-coding RNA (lncRNA)-microRNA (miRNA)-messenger (mRNA) constructed. identified 141 FR-DEGs TLE-HS, enriched T-cell activation involved immune response signaling pathways related lipids atherosclerosis. Further WGCNA performed select 47 overlapping FR-DEGs, which significantly 13 biological processes 14 Kyoto Encyclopedia Genes Genomes (KEGG) pathways, including negative regulation apoptotic process ferroptosis. Four genes, namely PDK4, SMPD1, GPT2, METTL14, signature Moreover, ROC derived from four predicting had an area under curve (AUC) 0.988 0.929, respectively. Furthermore, lncRNA-miRNA-mRNA constructed 4 consisted 5 lncRNAs miRNAs. based on found be strong predictor they may represent valuable therapeutic targets

Language: Английский

Ferroptosis role in complexity of cell death: unrevealing mechanisms in Parkinson’s disease and therapeutic approaches DOI
Anupam Awasthi,

Kousik Maparu,

Shamsher Singh

et al.

Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Language: Английский

Citations

4
Reproductive toxicity of perfluorobutane sulfonate in zebrafish (Danio rerio): Impacts on oxidative stress, hormone disruption and HPGL axis dysregulation DOI

Jenila John Santhi,

Praveen Kumar Issac, Manikandan Velayutham

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2025, Volume and Issue: 289, P. 110122 - 110122

Published: Jan. 7, 2025

Language: Английский

Citations

1
Incorporating Fresh Durum Wheat Semolina Pasta Fortified with Cardoncello (Pleurotus eryngii) Mushroom Powder as a Mediterranean Diet Staple DOI Creative Commons
Maria Calasso,

Antonino De Lisi,

Arianna Ressa

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 284 - 284

Published: Feb. 27, 2025

Pasta made from durum wheat semolina has a medium-high glycemic index score, high starch digestibility, and limited nutritional value due to its low fiber, vitamin, bioactive compound content. This study aimed enhance pasta's functional qualities by incorporating Pleurotus eryngii (PE) powder at various substitution levels achieve one claim least. research involved two phases: evaluating the chemical/physical, nutritional, functional, sensory properties of laboratory-scale samples validating selected formulations through industrial-scale production shelf-life analyses. The pasta sample with 8.62% PE (SPE8-P) demonstrated significantly improved qualities, including fiber content sufficient for "high content" claim, potential prebiotic activity indicated increased bifidobacterial density during simulated fecal microbiota fermentation. Despite enhanced riboflavin antioxidant content, regulatory constraints inclusion claims vitamin B2 richness activity. Although affecting color, taste, odor profiles, analysis revealed overall acceptability, supporting product's consumer acceptance. confirms feasibility producing innovative, nutritionally enriched as ingredient. Future will focus on in vivo evaluation establish classifying this prototype food.

Language: Английский

Citations

1
Micronutrient–Antioxidant Therapy and Male Fertility Improvement During ART Cycles DOI Open Access
Marwa Lahimer,

Severine Capelle,

Élodie Lefranc

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(2), P. 324 - 324

Published: Jan. 17, 2025

Today, accumulating evidence highlights the impact of oxidative stress (OS) on semen quality. It is considered to be a key factor contributing decline in male fertility. OS detected 30-80% men with infertility, highlighting its strong association impaired reproductive function and clinical outcomes following use assisted technologies. Spermatozoa are particularly vulnerable damage due their high content polyunsaturated fatty acids (PUFAs) limited antioxidant defense abilities. arises from an imbalance between production reactive oxygen species capacity neutralize or repair adverse effects. Evidence indicates that leads lipid peroxidation, protein oxidation, mitochondrial dysfunction, genomic instability. Micronutrient-antioxidant therapies can play role infertility improvement by neutralizing free radicals preventing cellular damage. Many different micronutrients, including L-carnitine, L-glutathione, coenzyme Q10, selenium, zinc, as well vitamins complexes, proposed improve sperm parameters fertility potential. This study aims review supplementation parameters, volume, motility, concentration, morphology, genome integrity (maturity fragmentation), vitro fertilization (IVF) outcomes. Antioxidant intake balanced lifestyle reduce enhancing spermatogenesis spermiogenesis processes, improving quality, protecting DNA integrity.

Language: Английский

Citations

0
Vitamin E Mitigates Polystyrene-Nanoplastic-Induced Visual Dysfunction in Zebrafish Larvae DOI Open Access
Febriyansyah Saputra, Azzah Dyah Pramata, Agoes Soegianto

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1216 - 1216

Published: Jan. 30, 2025

Vitamin E (VitE), a potent antioxidant, has demonstrated significant potential in mitigating oxidative stress and cellular damage, making it valuable agent for countering environmental toxicities, including those caused by polystyrene nanoplastics (PSNPs). This study examined the effects of PSNPs on zebrafish visual system evaluated protective role VitE. Zebrafish embryos were exposed to (0.01, 0.1, 1, 10 μg/mL) with or without 20 μM VitE co-treatment from fertilization 6 days post-fertilization (dpf). Visual function, morphology, molecular responses assessed at 4 dpf. Exposure concentrations 0.1 μg/mL significantly increased bioaccumulation eye concentration-dependent manner disrupted system. These disruptions reduction eye-to-body length ratio decreased optomotor response positivity swimming distance, indicating impaired function behavior. Furthermore, elevated reactive oxygen species (ROS) levels, induced retinal apoptosis, gene expression related development (six6, pax2, pax6a, pax6b), apoptosis (tp53, casp3, bax, bcl2a), antioxidant defense (sod1, cat, gpx1a). mitigated these adverse effects, reducing restoring defenses, preserving function. highlights as against PSNP-induced dysfunction underlines urgent need address nanoplastic pollution protect aquatic ecosystems.

Language: Английский

Citations

0
Neuronal Injury after Ischemic Stroke: Mechanisms of Crosstalk Involving Necroptosis DOI

X. Y. Zhang,

Hongyu Li, Yaowei Zhao

et al.

Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(1)

Published: Feb. 4, 2025

Language: Английский

Citations

0
Evaluation of the Impact of Selected Metallic Contaminants on Wild Rutilus rutilus Through Integrated Antioxidant Biomarker Responses DOI
Katarina Jovičić, Vesna Đikanović, Marina Radenković

et al.

Archives of Environmental Contamination and Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

Language: Английский

Citations

0
Na+/K+-ATPase: a multifunctional target in type 2 diabetes and pancreatic islets DOI Creative Commons
Lisha Mou, Zhenkun Fu,

Tony Bowei Wang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 19, 2025

Type 2 diabetes (T2D) is a widespread metabolic disorder marked by hyperglycemia, arising from insulin resistance and relative deficiency. This review investigates the critical role of Na+/K+-ATPase (NKA), transmembrane protein essential for maintaining cellular ion gradients, in pathophysiology T2D. We provide an overview NKA's biological functions, emphasizing its involvement signaling pathways, secretion, glucose homeostasis. The potential NKA as therapeutic target T2D analyzed, showcasing innovative strategies such activators, gene therapy, stem cell therapy aimed at enhancing activity to achieve better glycemic control. Additionally, multifunctional viability modulating immune responses islet transplantation may offer benefits improving transplant outcomes. By elucidating complex interactions between T2D, this aims shed light on developing novel interventions that meet multifaceted needs individuals suffering chronic condition, ultimately their health

Language: Английский

Citations

0
Neuroprotective insights into epigallocatechin gallate (EGCG) for neurodegenerative disorders DOI Creative Commons
Neha Kamboj,

S.D. Sharma,

Rahul Kumar

et al.

Exploration of neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Neurodegenerative disorders, including Alzheimer’s, Parkinson’s, Huntington’s, and amyotrophic lateral sclerosis, are among the most significant health concerns worldwide, characterized by neuronal dysfunction, oxidative stress, neuroinflammation, protein misfolding. Epigallocatechin gallate, a green tea polyphenol, has been reported to possess multifaceted neuroprotective properties. It reduces stress through free radical scavenging, activation of antioxidant enzymes, stabilization mitochondrial function. also inhibits neuroinflammation modulation key signaling pathways. suppresses amyloid-beta aggregation in Alzheimer’s alpha-synuclein fibrillation thus attenuating toxic accumulation. Its activity induction autophagy promotion synaptic plasticity supports survival However, low bioavailability metabolic instability hinder its translation into clinic. Strategies nanoparticle encapsulation, structural modifications, combination therapies being explored overcome these challenges. Future research could establish epigallocatechin gallate as viable candidate for managing neurodegenerative disorders.

Language: Английский

Citations

0
Fipronil Triggers Immunotoxicity Through Reactive Oxygen Species-Driven Mitochondrial Apoptosis in Thymocytes DOI Creative Commons

Jui-Fang Kuo,

Yai-Ping Hsiao,

Y.-S. Wang

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(3), P. 204 - 204

Published: March 12, 2025

Fipronil (FPN), a widely used pesticide, is associated with significant immunotoxic effects, particularly impacting thymocyte survival and immune homeostasis. This study explores the mechanistic pathways underlying FPN-induced apoptosis oxidative stress. Short-term FPN exposure (1–10 mg/kg) notably suppressed expression of both anti-apoptotic (Bcl-2, Bcl-6, Mcl-1) pro-apoptotic (Bnip3, Bim) genes in thymic tissues vivo. Additionally, isolated primary thymocytes, directly decreased Bcl-2, Mcl-1, Bnip3 expression, coupled increase Bim dose-dependent manner. treatment led to elevated reactive oxygen species (ROS), lipid peroxidation, mitochondrial membrane depolarization, reduced cellular metabolic activity, depleted intracellular calcium glutathione (GSH) levels, indicating dysfunction Annexin V/PI staining confirmed that induced late-stage necrosis thymocytes. These findings elucidate effects on highlighting its detrimental impact system integrity, development, T cell maturation through damage mitochondrial-mediated apoptosis.

Language: Английский

Citations

0