A data-driven approach for predicting the impact of drugs on the human microbiome

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: June 17, 2023

Abstract Many medications can negatively impact the bacteria residing in our gut, depleting beneficial species, and causing adverse effects. To guide personalized pharmaceutical treatment, a comprehensive understanding of various drugs on gut microbiome is needed, yet, to date, experimentally challenging obtain. Towards this end, we develop data-driven approach, integrating information about chemical properties each drug genomic content microbe, systematically predict drug-microbiome interactions. We show that framework successfully predicts outcomes in-vitro pairwise drug-microbe experiments, as well drug-induced dysbiosis both animal models clinical trials. Applying methodology, map large array interactions between pharmaceuticals human demonstrate medications’ anti-microbial are tightly linked their This computational has potential unlock development medicine microbiome-based therapeutic approaches, improving minimizing side

Language: Английский

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Characterization, degradation pathway and microbial community of aerobic granular sludge with ciprofloxacin at environmentally relevant concentrations

Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 205, P. 109255 - 109255

Published: Feb. 10, 2024

Language: Английский

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Gut Microbiota Comparison Between Intestinal Contents and Mucosa in Mice With Repeated Stress-Related Diarrhea Provides Novel Insight

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Feb. 23, 2021

Repeated stress-related diarrhea is a kind of functional bowel disorders (FBDs) that are mainly stemming from dysregulation the microbiota–gut–brain axis mediated by complex interplay 5-hydroxytryptophan (5-HT). Intestinal content and intestinal mucosa microbiota belong to two different community systems, role systems in repeated remains largely unknown. In order ascertain difference composition potential function between response on diarrhea, we collected contents mice with for 16S rRNA PacBio SMRT gene full-length sequencing, digital modeling method bacterial species abundance, correlations among serum 5-HT concentration were analyzed. We found microbiotal differences both consistent throughout all phylogenetic ranks, an increasing level resolution. Compared microbiota, diversity colonized more sensitive diarrhea. The PICRUSt2 metagenomic analysis likely perturb functions involved neural response. further mucosal microbiota-based relative abundance model was predictive methods machine-learning established multivariate dimensionality reduction ( R 2 = 0.876). These findings suggest might serve as novel prediction involvement addition focusing its mechanism gastrointestinal dysfunction.

Language: Английский

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The orchestra of human bacteriome by hormones

Microbial Pathogenesis, Journal Year: 2023, Volume and Issue: 180, P. 106125 - 106125

Published: April 27, 2023

Language: Английский

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Insight into the Potential of Somatostatin Vaccination with Goats as a Model: From a Perspective of the Gastrointestinal Microbiota

Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 728 - 728

Published: March 4, 2025

Deciphering the gastrointestinal microbial response to oral SS DNA vaccines with different doses is helpful for identifying mechanism effective utilization of vaccine improving animal production. Here, we conduct a comparative study (control: empty plasmid; low dose: 1 × 107 CFU vaccine; high 1012 vaccine) using goat as case investigate potential somatostatin vaccination from entire microbiota perspective. Our results show that body weight gain and slaughter rate are greater in L_SS group than C_SS group. Compared group, GH concentration reduced, while elevated cecum goats. Moreover, SCFAs goats, acetate molar proportion lower rumen, decreased, propionate increased data indicate low-dose possesses more efficient improvement productivity emphasizing dosage should be considered reach its optimal effect on host. find select distinct communities tract. Beta diversity analysis shows significant interaction. Microorganisms capable converting nutrients, including Ruminococcacease, Butyrivibrio, Akkermansia, Lachnospiraceae enriched, altering fermentation ruminants. correlation revealing these biomarkers have close association phenotypes productivity. These imply immunoneutralization might directly alter tract commensal bacterial structure, homeostasis, and, thus, modifying fermentability effected hormone level improve extends understanding regulation ruminants' growth through

Language: Английский

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Advantages of the falciform ligament separating method in pancreaticoduodenectomy for management of postoperative pancreatic fistula

Published: May 13, 2025

Background To evaluate the efficacy of falciform ligament separating (FaLS) method, where was used to separate space between pancreaticojejunostomy (PJ) and hepatic, artery in pancreaticoduodenectomy (PD) for postoperative pancreatic fistula (POPF) management. Methods Patients who underwent PD at Osaka Medical Pharmaceutical University Hospital from January 2018 June 2024 were retrospectively reviewed. The harvested advance placed PJ hepatic artery. spaces above below named “Area PJ” hepaticojejunostomy (HJ),” respectively. An abdominal drain each area. amylase concentration drainage fluid evaluated postoperatively, incidences POPF postpancreatectomy hemorrhage (PPH) analyzed. Results Grade B C POPFs PPH 21.4%, 0.9%, 3.5%, Two cases (0.9%) had a pseudoaneurysm gastroduodenal (GDA) stump. There significant difference two sites. HJ removed significantly earlier than drain. In cohort, clearance collection Area easily achieved by inserting an extra through anterior abdomen. Conclusions FaLS method could lower incidence GDA Early removal localization leaked juice are noteworthy benefits method.

Language: Английский

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Mechanical Bowel Preparation Is a Risk Factor for Postoperative Delirium as It Alters the Gut Microbiota Composition: A Prospective Randomized Single-Center Study

Frontiers in Aging Neuroscience, Journal Year: 2022, Volume and Issue: 14

Published: April 4, 2022

Postoperative delirium (POD) is a frequent complication in patients undergoing gastrectomy. Increasing evidence suggests that abnormal gut microbiota composition may contribute to its morbidity. However, it unclear whether mechanical bowel preparation would cause postoperative by altering the of patients. This study aimed investigate association between and gastrectomy.A prospective randomized single-center was performed. A total 81 with gastric cancer were enrolled randomly assigned two groups: group non-preparation according patient received MBP before surgery. To diagnose delirium, we used 3-Min Diagnostic Interview for Confusion Assessment Method-defined five successive days after 16s rRNA gene sequencing changes intestinal bacteria. The linear discriminant analysis effect size (LefSe) also identify different taxa fecal non-postoperative groups.We found there significant difference β-diversity (P = 0.048). Furthermore, had much higher rate (13/40, 32.5%) compared groups (4/41, 9.8%). Multivariate regression adjusted other risk factors indicated associated occurrence (odds ratio 4.792; 95% confidence interval: 1.274-18.028; P 0.020). When comparing without POD, Bacteroides Veillonella (genus), which group, < 0.05). Genus Olsenella both relatively non-POD 0.05).Mechanical not only altered but increased incidence delirium. Among all preparation, genus might be factor POD. beneficial bacteria reduce

Language: Английский

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Gut microbiota affects pancreatic fibrotic progression through immune modulation in chronic pancreatitis

Microbial Pathogenesis, Journal Year: 2023, Volume and Issue: 177, P. 106035 - 106035

Published: Feb. 23, 2023

Language: Английский

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Postoperative delayed gastric emptying: may gut microbiota play a role?

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 13, 2024

Postoperative delayed gastric emptying is a prevalent complication following surgical procedures, imposing heavy physical and financial burdens on patients. However, current treatment options remain suboptimal. In recent years, an increasing number of studies have highlighted that the gut microbiota its metabolites are closely associated with postoperative complications. Various factors can disrupt microbiome after surgery. This review discusses potential mechanisms by which their may contribute to pathogenesis emptying. knowledge base limited in terms fully understanding exact involved. It therefore evident further research required elucidate role emptying, aim uncovering new possibilities for preventive measures therapeutic treatments.

Language: Английский

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Prevention and Treatment of Grade C Postoperative Pancreatic Fistula

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(24), P. 7516 - 7516

Published: Dec. 19, 2022

Postoperative pancreatic fistula (POPF) is a troublesome complication after surgeries, and grade C POPF the most serious situation among fistulas. At present, incidence of varies from less than 1% to greater 9%, with an extremely high postoperative mortality rate 25.7%. The patients finally undergo surgery poor prognosis various failed conservative treatments. Although surgical perioperative attempts have been made reduce POPF, rates this costly not significantly diminished. Hearteningly, several related studies found that intra-abdominal infection intestinal flora could promote development which would help physicians better prevent complication. In review, we briefly introduced definition relevant risk factors for POPF. Moreover, review discusses two main pathways, direct juice spillover bacterial translocation, by microbes enter abdominal cavity. Based on abovementioned theory, summarize operation techniques management discuss novel methods treatments reverse dilemma.

Language: Английский

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