40 Hz Visual Stimulation During Sleep Evokes Neuronal Gamma Activity in NREM and REM Stages DOI Creative Commons
Laura Hainke, James Dowsett, Manuel Spitschan

et al.

SLEEP, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract Study Objectives Visual stimulation (VS) at 40 Hz is being tested as a non-invasive approach against dementias such Alzheimer’s disease. Applying it during sleep could increase the convenience, duration, and efficacy of stimulation. Here, we feasibility VS in proof-of-concept study. Methods Thirty healthy participants underwent one control experimental night polysomnography laboratory. was delivered wakefulness (W), NREM stages 2 3, REM sleep. Results As expected, EEG spectral power increased all four condition, compared to control. It highest W similar across 2, REM, with large medium effect sizes, respectively. Steady-state visually evoked potential (SSVEP) analyses time domain confirmed specificity effect. Secondary revealed that intervention did not impair objective subjective quality beyond first-night Conclusions effectively neuronal gamma activity frequency without degrading quality, supporting this approach. These findings lay groundwork for optimizing gamma-band sensory tool causally study cognitive functions scalable, dementias.

Language: Английский

40 Hz light flickering facilitates the glymphatic flow via adenosine signaling in mice DOI Creative Commons

Xiaoting Sun,

Liliana Dias,

Chenlei Peng

et al.

Cell Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Aug. 6, 2024

Abstract The glymphatic-lymphatic system is increasingly recognized as fundamental for the homeostasis of brain milieu since it defines cerebral spinal fluid flow in parenchyma and eliminates metabolic waste. Animal human studies have uncovered several important physiological factors regulating glymphatic including sleep, aquaporin-4, hemodynamic factors. Yet, our understanding modulation limited, which has hindered development glymphatic-based treatment aging neurodegenerative disorders. Here, we present evidence from fluorescence tracing, two-photon recording, dynamic contrast-enhanced magnetic resonance imaging analyses that 40 Hz light flickering enhanced influx efflux independently anesthesia an effect attributed to increased astrocytic aquaporin-4 polarization vasomotion. Adenosine-A 2A receptor (A R) signaling emerged neurochemical underpinning flickering-induced enhancement flow, based on cerebrofluid adenosine levels, abolishment by pharmacological or genetic inactivation equilibrative nucleotide transporters-2 A R, physical functional R–aquaporin-4 interaction astrocytes. These findings establish a novel non-invasive strategy with translational potential relieve

Language: Английский

Citations

14
Optochemical control of slow-wave sleep in the nucleus accumbens of male mice by a photoactivatable allosteric modulator of adenosine A2A receptors DOI Creative Commons
Koustav Roy, Xuzhao Zhou, Rintaro Otani

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 30, 2024

Optochemistry, an emerging pharmacologic approach in which light is used to selectively activate or deactivate molecules, has the potential alleviate symptoms, cure diseases, and improve quality of life while preventing uncontrolled drug effects. The development in-vivo applications for optochemistry render brain cells photoresponsive without relying on genetic engineering been progressing slowly. nucleus accumbens (NAc) a region regulation slow-wave sleep (SWS) through integration motivational stimuli. Adenosine emerges as promising candidate molecule activating indirect pathway neurons NAc expressing adenosine A

Language: Английский

Citations

7
Synchronized neuronal activity drives waste fluid flow DOI
Lauren M. Hablitz, Maiken Nedergaard

Nature, Journal Year: 2024, Volume and Issue: 627(8002), P. 44 - 45

Published: Feb. 28, 2024

Language: Английский

Citations

6
Longitudinal Effects of Lifetime Caffeine Consumption on Levels of Depression, Anxiety, and Stress: A Comprehensive Review DOI Creative Commons
Sena Ünsal, Nevin Şanlıer

Current Nutrition Reports, Journal Year: 2025, Volume and Issue: 14(1)

Published: Feb. 1, 2025

Abstract Purpose of Review Caffeine has high bioavailability and a purine-like alkaloid structure. It exerts wide-ranging physiological effects by binding to adenosine receptors throughout the human body. Through activation those receptors, it can regulate many events in The impact caffeine consumption on depression, anxiety, stress, health remains unclear, constituting an important knowledge gap. This review was conducted examine stress levels offer some recommendations for its future use. Recent Findings We performed comprehensive literature search using PubMed, Web Science Google Scholar databases original articles published recent years “caffeine metabolism”, mechanism”, “anxiety”, “depression”, “stress”. Caffeine, which antagonistic effect adenosine, reduce risk symptoms depression improve general mental modulating central nervous system neurotransmitter systems. However, increases anxiety levels, are often seen together with observed due high-dose consumption. Summary Caffeine’s may vary depending different factors, but level is particularly attention should be paid upper limits reference values while evaluating amounts.

Language: Английский

Citations

0
In Situ Mass Spectrometry Imaging to Elucidate the Effects of an Adenosine A2A Receptor Agonist and Alprazolam on Sleep Regulation DOI Creative Commons
Yi Zhang,

Guixiang Yang,

Qian Jin

et al.

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: 8(3), P. 841 - 853

Published: Feb. 20, 2025

Alprazolam (Alp), a commonly used sleep medication in clinical practice, has several potential limitations, including narrow therapeutic dosage range and delayed onset. CGS21680 (CGS), selective agonist of the adenosine A2A receptor, exhibits neuroinhibitory properties. This study aimed to evaluate effects CGS on properties Alp. The sleep-inducing Alp were assessed through righting reflex, while sedative evaluated by spontaneous activity detection. synergistic effect was using electroencephalography electromyography. results indicate that we optimized selected ED5 dose ED50 for coadministration. reduced latency induced extended duration. distribution brain mass spectrometry imaging (MSI). blood-brain barrier (BBB) model established impact transmittance indicated influenced across various regions increased Alp's BBB. metabolic pathways GABA, glutamate, glutamine MSI enzyme verification. coadministration resulted regulation during maintenance periods, respectively. In conclusion, potentiating is attributed its ability modulate enhancing BBB permeability influence Alp-induced neurotransmitter release.

Language: Английский

Citations

0
Rhythmic gamma frequency light flickering ameliorates stress-related behaviors and cognitive deficits by modulating neuroinflammatory response through IL-12-Mediated cytokines production in chronic stress-induced mice DOI

Junqi Yao,

Liming Zhang,

Chunkui Zhang

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 121, P. 213 - 228

Published: July 21, 2024

Language: Английский

Citations

2
Treating insomnia with 40 Hz light flicker DOI Creative Commons
P. Lorenzo Bozzelli, Li‐Huei Tsai

Cell Research, Journal Year: 2024, Volume and Issue: 34(5), P. 333 - 334

Published: March 8, 2024

A new study published in Cell Research reports that 40 Hz light flicker induces a somnogenic effect mediated by adenosine-ENT2 signaling.Remarkably, was shown to improve the sleep quality of children with insomnia, offering potential non-pharmacological therapeutic approach for treatment disorders.Sleep disruptions are prevalent various brain disorders, yet field's limited understanding underlying mechanisms has hindered advancement effective therapies. 1Furthermore, emerging evidence implicated disturbances as drivers disease, alterations often preceding disease onset several years, 1 highlighting importance developing therapies.The exploration sensory stimulation garnered great interest non-invasive neurodegenerative diseases. 2In from Zhou et al., 3 authors sought identify through which exerts protective effect.The focused on adenosine signaling given gamma oscillatory activity is an energetically demanding process requiring ATP metabolism results increased extracellular adenosine.Adenosine known reduce neuronal activity, and accumulation increases pressure. 4s such, it blockade receptors underlies wakefulness-promoting effects caffeine. 4o examine levels response frequencies, used G protein-coupled receptor (GPCR) activation-based sensor (GRAB Ado ) regions.When compared other frequencies were tested primary visual cortex (V1), resulted maximal levels, sustained hours after cessation stimulation.Additional experiments identified cellular source be both excitatory inhibitory neurons, but not astrocytes, suggesting subtypes interact generate adenosine, similar interactions giving rise oscillations. 5n microglia have recently been tightly regulate signaling, providing feedback neurons CD73-dependent conversion AMP adenosine. 6Zhou al. found unaltered CD73-knockout (KO) mouse, alternate pathway involved flicker-induced increase 2iven intracellular can also directly effluxed via ENT1/2 transmembrane efflux transporters,

Language: Английский

Citations

1
Study on Gamma sensory flicker for Insomnia DOI

Yakun Liu,

Xinrong Li, Sha Liu

et al.

International Journal of Neuroscience, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: April 17, 2024

Objectives Insomnia has been the subject of much systematic research because it is a risk factor for variety diseases. There some evidence that gamma sensory stimulation therapy also demonstrated to improve sleep quality people with Alzheimer's disease. However, unclear whether this method effective treating insomnia. The principal objective project was investigate efficacy and safety flicker in improving insomnia patients.

Language: Английский

Citations

1
Enhancement of spatial learning by 40 Hz visual stimulation requires parvalbumin interneuron-dependent hippocampal neurogenesis DOI Creative Commons
Hai Yan,

Xufan Deng,

Yunxuan Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

SUMMARY Acute and short-term rhythmic 40 Hz light flicker stimulation has shown promising results in alleviating cognitive impairments mouse models of Alzheimer’s disease (AD), stroke, autism spectrum disorders (ASD). Understanding the long-term impacts underlying mechanisms is crucial to progress this approach for potential human therapeutic applications. Here, we show that prolonged exposure (1 hour per day 30 days) significantly improved spatial learning neurogenesis dentate gyrus (DG) without harmful behavioral side effects. Mice with transgenic deletion doublecortin-positive cells (DCX DTR ) adult hippocampus failed exhibit enhanced stimulation. Inactivation or knockout GABAergic parvalbumin (PV) interneurons reduced effects entrainment enhancement. Mechanistically, did not alter regional microvessel blood flow but raised PV excitability GABA levels inhibitory transmission DG. Blocking A receptors reversed improvements neurogenesis. These data showed enhances through PV-dependent neurogenesis, which requires elevated as a critical neurochemical mechanism sustaining In brief Rhythmic alleviates diverse animal neurological diseases. its vital advancing humans. improves boosts gyrus. Activation support newborn immature neurons underlie effect, demonstrating lasting benefits treatment Highlights Long-term hippocampus, devoid adverse The evoked activity interneurons. Increased serves

Language: Английский

Citations

1
Adenosine Metabolism Pathway Alterations in Frontal Cortical Neurons in Schizophrenia DOI Creative Commons
Smita Sahay,

Emily A. Devine,

C. Vargas

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1657 - 1657

Published: Oct. 6, 2024

Schizophrenia is a neuropsychiatric illness characterized by altered neurotransmission, in which adenosine, modulator of glutamate and dopamine, plays critical role that relatively unexplored the human brain. In present study, postmortem brain tissue from anterior cingulate cortex (ACC) individuals with schizophrenia (

Language: Английский

Citations

1