tRNA selectivity during ribosome-associated quality control regulates the critical sterility-inducing temperature in two-line hybrid rice

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(6)

Published: Feb. 6, 2025

The two-line hybrid rice system, a cutting-edge breeding technology, has greatly boosted global food security. In thermo-sensitive genic male sterile (TGMS) lines, the critical sterility-inducing temperature (CSIT; at which TGMS lines change from fertile to complete sterile) acts as key threshold. We recently uncovered that sterility 5 ( tms5 ), locus presenting in over 95% of leads overaccumulation 2′,3′-cyclic phosphate (cP)-ΔCCA-tRNAs and deficiency mature tRNAs, underlies molecular mechanism -mediated TGMS. However, there are few reports on regulatory controlling CSIT. Here, we identified suppressor , an amino acid substitution (T552I) Rqc2 (ribosome-associated quality control 2), increases CSIT through its C-terminal alanine threonine modification (CATylation) activity. This alters tRNA selectivity, leading recruitment different tRNAs A-site ribosome CATylation rate by OsRqc2 during ribosome-associated (RQC), process rescues stalled ribosomes degrades abnormal nascent chains translational elongation. Further, mutation restores levels tRNA-Ser/Ile increase lines. Our findings reveal origin overaccumulated cP-ΔCCA-tRNAs further deepening our understanding network governing containing tms5.

Language: Английский

---

核糖体介导的翻译调控与植物环境温度适应

Chinese Science Bulletin (Chinese Version), Journal Year: 2025, Volume and Issue: 70(7), P. 807 - 815

Published: Feb. 8, 2025

---

The E3 ligase OsHel2 impedes readthrough of stalled mRNAs to regulate male fertility in thermo-sensitive genic male sterile rice

Plant Communications, Journal Year: 2024, Volume and Issue: 6(2), P. 101192 - 101192

Published: Nov. 13, 2024

Heterosis is extensively used in the 2-line hybrid breeding system. Photosensitive/thermosensitive genic male sterile (P/TGMS) lines are key components of rice, and TGMS containing tms5 have significantly advanced rice breeding. We cloned TMS5 gene found that a tRNA cyclic phosphatase can remove 2',3'-cyclic phosphate (cP) from cP-ΔCCA-tRNAs for efficient repair to ensure maintenance mature levels. mutation causes increased levels reduced tRNAs, leading sterility at restrictive temperatures. However, regulatory network tms5-mediated remains be clarified. Here, we demonstrate E3 ligase OsHel2 cooperates with regulate Consistently, both accumulation reduction tRNAs mutant largely recovered oshel2-1 mutant. A lesion results partial readthrough stalled sequences, thereby enabling evasion ribosome-associated protein quality control (RQC) surveillance. Our findings reveal mechanism by which impedes mRNA sequences fertility providing paradigm investigating how disorders RQC pathway impair cellular functions lead diseases or defects other organisms.

Language: Английский

---

Exploiting male sterility toward the development of hybrid rice

Seed Biology, Journal Year: 2024, Volume and Issue: 3(1), P. 0 - 0

Published: Jan. 1, 2024

Language: Английский

---

tRNA repair: the key to thermo-sensitive male sterility in rice

Cell Research, Journal Year: 2024, Volume and Issue: 34(11), P. 755 - 756

Published: Sept. 16, 2024

Language: Английский

---

Ribosome-associated quality control underlies rice thermo-sensitive genic male sterility

Molecular Plant, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

---