Influence of diverse storage conditions of double‐stranded RNAin vitro on the RNA interference efficiency in vivo insect Tribolium castaneum DOI
Mei Bai,

Zi‐Ling Liu,

Yu‐Yu Zhou

et al.

Pest Management Science, Journal Year: 2022, Volume and Issue: 79(1), P. 45 - 54

Published: Sept. 10, 2022

A significant variation in RNA interference (RNAi) efficiency hinders further functional gene studies and pest control application many insects. The available double-stranded (dsRNA) molecules introduced into the target cells are regarded as crucial factor for efficient RNAi response. However, numerous have only focused on dsRNA stability vivo; it is uncertain whether different storage conditions vitro play a role variable among insects.A marker cardinal, which leads to white eyes when knocked-down red flour beetle Tribolium castaneum, was used evaluate effects of under conditions. We demonstrated that molecule very stable typical cryopreservation temperatures (-80 -20 °C) within 180 days, shows no differences either low temperature. Unexpectedly, while were treated with multiple freeze-thaw cycles up 50 times between -80/-20 °C room temperature, we discovered integrity comparable fresh dsRNA. Finally, measured refrigerated (4 °C), surprisingly found still days can induce an response initial dsRNA.Our results indicate extraordinarily various temperature did not significantly impact vivo © 2022 Society Chemical Industry.

Language: Английский

Tribolium castaneum: A Model Insect for Fundamental and Applied Research DOI Open Access
James F. Campbell, Christos G. Athanassiou,

David W. Hagstrum

et al.

Annual Review of Entomology, Journal Year: 2021, Volume and Issue: 67(1), P. 347 - 365

Published: Oct. 6, 2021

Tribolium castaneum has a long history as model species in many distinct subject areas, but improved connections among the genetics, genomics, behavioral, ecological, and pest management fields are needed to fully realize this species' potential model. was first beetle whose genome sequenced, new assembly enhanced annotation, combined with readily available genomic research tools, have facilitated its increased use wide range of functional genomics research. Research into T. castaneum's sensory systems, response pheromones kairomones, patterns movement landscape utilization our understanding behavioral ecological processes. also been development monitoring tactics, including evaluation insecticide resistance mechanisms. Application approaches is infancy offers powerful tool that can link mechanism function facilitate exploitation these relationships better manage important food pest.

Language: Английский

Citations

85
The red flour beetle T. castaneum: elaborate genetic toolkit and unbiased large scale RNAi screening to study insect biology and evolution DOI Creative Commons
Martin Klingler, Gregor Bucher

EvoDevo, Journal Year: 2022, Volume and Issue: 13(1)

Published: July 19, 2022

Abstract The red flour beetle Tribolium castaneum has emerged as an important insect model system for a variety of topics. With respect to studying gene function, it is second only the vinegar fly D. melanogaster . RNAi response in T. exceptionally strong and systemic, appears target all cell types processes. Uniquely emerging organisms, offers opportunity performing time- cost-efficient large-scale screening, based on commercially available dsRNAs targeting genes, which are simply injected into body cavity. Well established transgenic genome editing approaches met by ease husbandry relatively short generation time. Consequently, number tools like UAS/Gal4, Cre/Lox , imaging lines enhancer trap already available. been genetic experimental decades now become workhorse molecular reverse genetics well vivo imaging. Many aspects development general biology more insect-typical this compared Thus, orthologs well-described genes allowed macro-evolutionary comparisons developmental processes such axis formation, segmentation, appendage, head brain development. Transgenic have opened new ways Moreover, first choice research that not represented fly, or difficult study there, e.g. extraembryonic tissues, cryptonephridial organs, stink gland dsRNA-based pesticides.

Language: Английский

Citations

41
Anatomical changes of Tenebrio molitor and Tribolium castaneum during complete metamorphosis DOI Creative Commons
Maria Luigia Vommaro, Sandro Donato, Simone Caputo

et al.

Cell and Tissue Research, Journal Year: 2024, Volume and Issue: 396(1), P. 19 - 40

Published: Feb. 27, 2024

In holometabolous insects, extensive reorganisation of tissues and cells occurs at the pupal stage. The remodelling external exoskeleton internal organs that intervenes during metamorphosis has been traditionally studied in many insect species based on histological or ultrastructural methods. This study demonstrates use synchrotron X-ray phase-contrast micro-computed tomography as a powerful, non-destructive tool for situ morphological observation anatomical structures stage two Tenebrionid beetles, i.e. Tribolium castaneum Tenebrio molitor, known important pests, well emerging promising models experimental biology. Virtual sections three-dimensional reconstructions were performed both males females early, intermediate, late dataset allowed us to observe gut nervous system shaping female male reproductive different ages mealworm red flour beetles. Moreover, we observed timing duration pattern organ development varied between analysed, likely related species-specific adaptations pre-imaginal stages environmental conditions, which ultimately affect their life cycle. research provides new knowledge modifications occur insects baseline set information beetle may support future forensics, physiology, ecology an image atlas educational purposes.

Language: Английский

Citations

10
BeetleAtlas: An Ontogenetic and Tissue-specific Transcriptomic Atlas of the Red Flour Beetle Tribolium castaneum DOI Creative Commons
David P. Leader, Muhammad Tayyib Naseem, Kenneth A. Halberg

et al.

Journal of Molecular Biology, Journal Year: 2024, Volume and Issue: 436(17), P. 168520 - 168520

Published: March 6, 2024

The red flour beetle Tribolium castaneum has emerged as a powerful model in insect functional genomics. However, major limitation the field is lack of detailed spatio-temporal view genetic signatures underpinning function distinct tissues and life stages. Here, we present an ontogenetic tissue-specific web-based resource for transcriptomics — BeetleAtlas (https://www.beetleatlas.org). This web application provides access to database populated with quantitative expression data nine adult seven larval tissues, well four embryonic stages Tribolium. allows one search individual genes obtain values both total gene enrichment different together isoforms. To facilitate cross-species studies, can also use Drosophila melanogaster identifiers related genes. For retrieved there are options identify display tissue or homologous Five additional modes available find conforming any following criteria: exhibiting high particular tissue; showing significant differences between larva adult; having peak at specific stage development; belonging category; displaying pattern similar that query gene. We illustrate how feaures be used illuminate our understanding mechanisms biology what largest animal group on earth.

Language: Английский

Citations

4
Sexual selection matters in genetic rescue, but productivity benefits fade over time: a multi-generation experiment to inform conservation DOI Creative Commons
G. S. West, Michael D. Pointer, Will Nash

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2025, Volume and Issue: 292(2039)

Published: Jan. 1, 2025

Globally, many species are threatened by population decline because of anthropogenic changes leading to fragmentation, genetic isolation and inbreeding depression. Genetic rescue, the controlled introduction variation, is a method used relieve such effects in small populations. However, without understanding how characteristics rescuers impact rescue attempts interventions run risk being sub-optimal, or even counterproductive. We use red flour beetle ( Tribolium castaneum ) test rescuer sex, sexual selection background, on productivity. record productivity 24 36 replicated populations for ten generations following intervention. find little no sex efficacy but show that background elevated makes individuals more effective rescuers. In both experiments, diminish 6–10 after rescue. Our results confirm can be influenced level rescuing an important factor. any increase fitness associated with may last limited number generations, suggesting implications conservation policy practice.

Language: Английский

Citations

0
Impact of essential oils on enzymes activity, reserve products, biomarkers, and gene expression of Tribolium castaneum DOI Creative Commons
Houssam Annaz, Francesco Cacciola,

Ayoub Kounnoun

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2025, Volume and Issue: 293, P. 110167 - 110167

Published: March 2, 2025

Language: Английский

Citations

0
Some corners are more equal than others: Beetles linger longer in acute‐angled corners than in obtuse‐angled ones DOI Creative Commons

K Hanna,

A. Tsveiman,

Inon Scharf

et al.

Journal of Zoology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract The ability of animals to find shelter is vital for survival, providing protection from predators and harsh conditions. In lab studies, often favor walls corners, suggesting a natural inclination toward enclosed spaces safety reasons. Here, we investigated how characteristics, specifically its size corner angles, affect usage by the red flour beetle, Tribolium castaneum , cosmopolitan pest stored products, as well beetle's movement. beetles remained longer in narrow shelters than medium‐sized shelters, with no significant difference wide shelters. They spent less time obtuse‐angled corners right‐angled ones, more acute‐angled indicating their differentiate among choose suitable ones. Next, examined whether movement through corridor delayed strongly presence obstacles forming acute angles obtuse angles. Although there was proportion arrivals at destination cell or required cross corridor, caused higher frequency reentries into origin cell, possibly due beetles' tendency follow walls. This research may have applied importance, such enhancing our understanding beetle movement, strategies delay arrival new areas, optimal placement traps.

Language: Английский

Citations

0
Research on the Effect of Lamprey Sex Ratio on Ecosystem Diversity Based on Predator-Prey Model DOI

X. R. Lyu,

Yao Zhang, Lei Dai

et al.

Highlights in Science Engineering and Technology, Journal Year: 2025, Volume and Issue: 139, P. 144 - 152

Published: April 28, 2025

Predator-prey models are valuable in predicting population dynamics and revealing the feedback effects of factors such as sex ratio on ecosystem diversity. Specifically, this study innovatively constructed a small-scale differential equation model with quadratic correction term for ratio. It approximately linearly fitted functional relationship between food availability. Subsequently, several sets computer simulations were designed to iteratively under externally uncertain factors. The simulation results showed that biodiversity index H peaked at about 0.74 when reached 0. while possessed highest species richness. In addition, varied 0.56 0.78, number predators increased by an average 15% per generation proportion males increased, prey correspondingly decreased 10%. Meanwhile, Shannon-Wiener H-value nonlinearly increase less than 0.74, began decrease slowly exceeded 0.74. show has significant effect level

Language: Английский

Citations

0
scRNA-seq Reveals Novel Genetic Pathways and Sex Chromosome Regulation in Tribolium Spermatogenesis DOI Creative Commons
Michael Robben, Balan Ramesh, Shana H. Pau

et al.

Genome Biology and Evolution, Journal Year: 2024, Volume and Issue: 16(3)

Published: March 1, 2024

Abstract Spermatogenesis is critical to sexual reproduction yet evolves rapidly in many organisms. High-throughput single-cell transcriptomics promises unparalleled insight into this important process but understanding can be impeded nonmodel systems by a lack of known genes that reliably demarcate biologically meaningful cell populations. Tribolium castaneum, the red flour beetle, lacks markers for spermatogenesis found insect species like Drosophila melanogaster. Using sequencing data collected from adult beetle testes, we implement strategy elucidating populations using transient expression stage identification markers, weighted principal component clustering, and SNP-based haploid/diploid phasing. We identify correspond observable points sperm differentiation find specific each stage. Our results indicate molecular pathways underlying Coleoptera are substantially diverged those Diptera. also show most on X chromosome experience meiotic sex inactivation. Temporal MSL complex homologs coupled with spatial analysis potential chromatin entry sites further suggests dosage compensation machinery may mediate escape inactivation postmeiotic reactivation chromosome.

Language: Английский

Citations

3
3D Printed Organisms Enabled by Aspiration‐Assisted Adaptive Strategies DOI Creative Commons
Guebum Han, Kanav Khosla,

Kieran T. Smith

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 21, 2024

Abstract Devising an approach to deterministically position organisms can impact various fields such as bioimaging, cybernetics, cryopreservation, and organism‐integrated devices. This requires continuously assessing the locations of randomly distributed collect transfer them target spaces without harm. Here, aspiration‐assisted adaptive printing system is developed that tracks, harvests, relocates living moving on via a pick‐and‐place mechanism adapts updated visual spatial information about spaces. These strategies successfully positioned single static organism, multiple in droplets, organism Their capabilities are exemplified by vitrification‐ready cryoprotectant sorting live from dead ones, positioning curved surfaces, organizing organism‐powered displays, integrating with materials devices customizable shapes. ultimately lead autonomous biomanufacturing methods evaluate assemble for variety multi‐organism‐based applications.

Language: Английский

Citations

3