RNAi in the Red Flour Beetle (Tribolium Castaneum): Current Status and Future Perspectives

Published: Jan. 1, 2025

Language: Английский

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3D Printed Organisms Enabled by Aspiration‐Assisted Adaptive Strategies

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 21, 2024

Abstract Devising an approach to deterministically position organisms can impact various fields such as bioimaging, cybernetics, cryopreservation, and organism‐integrated devices. This requires continuously assessing the locations of randomly distributed collect transfer them target spaces without harm. Here, aspiration‐assisted adaptive printing system is developed that tracks, harvests, relocates living moving on via a pick‐and‐place mechanism adapts updated visual spatial information about spaces. These strategies successfully positioned single static organism, multiple in droplets, organism Their capabilities are exemplified by vitrification‐ready cryoprotectant sorting live from dead ones, positioning curved surfaces, organizing organism‐powered displays, integrating with materials devices customizable shapes. ultimately lead autonomous biomanufacturing methods evaluate assemble for variety multi‐organism‐based applications.

Language: Английский

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Traits Underlying Experimentally Evolved Dispersal Behavior in Tribolium castaneum

Journal of Insect Behavior, Journal Year: 2024, Volume and Issue: 37(3-4), P. 220 - 232

Published: Sept. 27, 2024

Language: Английский

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Endophytic Diaporthe as Promising Leads for the Development of Biopesticides and Biofertilizers for a Sustainable Agriculture

Microorganisms, Journal Year: 2022, Volume and Issue: 10(12), P. 2453 - 2453

Published: Dec. 12, 2022

Plant pathogens are responsible for causing economic and production losses in several crops worldwide, thus reducing the quality quantity of agricultural supplies. To reduce usage chemically synthesized pesticides, strategies approaches using microorganisms being used plant disease management. Most studies concerning plant-growth promotion biological agents to control diseases mainly focused on bacteria. In addition, a great portion registered commercialized biopesticides bacterial-based products. Despite fungal endophytes having been identified as promising candidates their use control, it is utmost importance develop improve existing knowledge this research field. The genus Diaporthe, encompasses pathogens, saprobes that have screened secondary metabolite, due polyketides variety unique bioactive metabolites with agronomic importance. Some these exhibit antifungal antibacterial activity controlling phytotoxic development potential mycoherbicides. Moreover, species Diaporthe reported biofertilizers. For reason, review we summarize produce natural products application agriculture describe benefits fungi promote host plant’s growth.

Language: Английский

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Population density affects sexual selection in an insect model

Functional Ecology, Journal Year: 2023, Volume and Issue: 37(10), P. 2734 - 2747

Published: July 31, 2023

Abstract Understanding the impact of demographic and ecological factors on strength sexual selection is pivotal to predict eco‐evolutionary dynamics in populations facing environmental change. Density expected show pervasive intra‐ interspecific variation natural populations, but its effect remains largely unexplored. We tested experimentally density standardized metrics male female red flour beetles. Specifically, we explored effects during mating by manipulating size groups arena. found that higher densities imposed a larger group amplified opportunity for pre‐copulatory females and, smaller extent, also males. Moreover, males, detected post‐copulatory groups. By contrast, differences arena resulted steeper gradient. Overall, these data suggest an important factor contributing tremendous within among species. Read free Plain Language Summary this article Journal blog.

Language: Английский

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A life-history allele of large effect shortens developmental time in a wild insect population

Nature Ecology & Evolution, Journal Year: 2023, Volume and Issue: 8(1), P. 70 - 82

Published: Nov. 13, 2023

Language: Английский

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Tests of evolutionary and genetic rescue using flour beetles, Tribolium castaneum, experimentally evolved to thermal conditions

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(5)

Published: April 29, 2024

Small, isolated populations are often characterised by low levels of genetic diversity. This can result in inbreeding depression and reduced capacity to adapt changes the environment, therefore higher risk extinction. However, sometimes these be rescued if allowed increase size or migrants enter, bringing new allelic variation thus increasing study uses experimental manipulation population migration quantify their effects on fitness a challenging environment better understand rescue. Using small, replicated

Language: Английский

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Generating and testing the efficacy of transgenic Cas9 in Tribolium castaneum

Insect Molecular Biology, Journal Year: 2022, Volume and Issue: 31(5), P. 543 - 550

Published: April 16, 2022

Abstract CRISPR/Cas9 genome editing has now expanded to many insect species, including Tribolium castaneum . However, compared Drosophila melanogaster , the CRISPR toolkit of T. is limited. A particularly apparent gap lack Cas9 transgenic animals, which generally offer higher efficiency. We address this by creating and testing beetles expressing Cas9. generated two different constructs bearing basal heat shock promoter‐driven Cas9, distinct 3′ UTRs, one containing fused EGFP a T2A peptide. Analyses activity in each line demonstrated that both designs are capable inducing CRISPR‐ mediated changes absence induction. Overall, these resources enhance accessibility for research community provide benchmark against compare future lines.

Language: Английский

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Therapeutic Use of Insects and Insect Products

Indian Journal of Entomology, Journal Year: 2023, Volume and Issue: unknown, P. 01 - 10

Published: June 19, 2023

Insects and their products have been used as resources of medicines all over the world since ancient times. Insect-derived compounds for therapeutic use huge promise they remedial, analgesic, antibacterial, diuretic, sedative, anticancer anti-rheumatic properties. In old traditional medicine, insect such honey, venom, maggots, silk cantharidine etc. a treatment many human ailments. Therapies based on insects are now finding increased acceptance importance because failure substitute drug delivery methods to deliver lead in key areas. There number new advancements development natural prospective medicines. This review provides updated information regarding modern across various parts world.

Language: Английский

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Genetic architecture of dispersal behaviour in the post-harvest pest and model organism Tribolium castaneum

Heredity, Journal Year: 2023, Volume and Issue: 131(4), P. 253 - 262

Published: July 29, 2023

Dispersal behaviour is an important aspect of the life-history animals. However, genetic architecture dispersal-related traits often obscure or unknown, even in well studied species. Tribolium castaneum a globally significant post-harvest pest and established model organism, yet studies its dispersal have shown ambiguous results basis this remains unresolved. We combine experimental evolution agent-based modelling to investigate number loci underlying T. castaneum, whether trait sex-linked. Our findings demonstrate rapid under selection. find no evidence sex-biases offspring crosses, supporting autosomal trait. Moreover, simulated data approximates scenarios where controlled by one few loci, but not many loci. Levels experimentally inbred lines, compared with simulations, indicate that single locus supported. Taken together, these lines support oligogenic castaneum. These implications for applied management our understanding coleoptera, world's most species-rich order.

Language: Английский

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