Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement DOI Creative Commons
Qiang Li, Manoj Sapkota, Esther van der Knaap

et al.

Horticulture Research, Journal Year: 2020, Volume and Issue: 7(1)

Published: March 15, 2020

Directed breeding of horticultural crops is essential for increasing yield, nutritional content, and consumer-valued characteristics such as shape color the produce. However, limited genetic diversity restricts amount crop improvement that can be achieved through conventional approaches. Natural changes in

Language: Английский

Rhizosphere microbiome: Engineering bacterial competitiveness for enhancing crop production DOI Creative Commons
Ashwani Kumar, Anamika Dubey

Journal of Advanced Research, Journal Year: 2020, Volume and Issue: 24, P. 337 - 352

Published: April 30, 2020

Plants in nature are constantly exposed to a variety of abiotic and biotic stresses which limits their growth production. Enhancing crop yield production feed exponentially growing global population sustainable manner by reduced chemical fertilization agrochemicals will be big challenge. Recently, the targeted application beneficial plant microbiome cocktails counteract stress is gaining momentum becomes an exciting frontier research. Advances next generation sequencing (NGS) platform, gene editing technologies, metagenomics bioinformatics approaches allows us unravel entangled webs interactions holobionts core microbiomes for efficiently deploying increase crops nutrient acquisition resistance stress. In this review, we focused on shaping rhizosphere susceptible host from resistant comprises specific type microbial community with multiple potential benefits CRISPR/Cas9 based strategies manipulation susceptibility genes plants improving health. This review significant providing first-hand information improve fundamental understanding process helps microbiome.

Language: Английский

Citations

266

First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts DOI Open Access

Sapna Badhan,

Andrew S. Ball, Nitin Mantri

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(1), P. 396 - 396

Published: Jan. 1, 2021

The current genome editing system Clustered Regularly Interspaced Short Palindromic Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and simplicity in several plant-based applications. Together with the availability of a vast amount data transcriptome data, CRISPR/Cas9 presents massive opportunity for plant breeders researchers. successful delivery ribonucleoproteins (RNPs), which are composed enzyme synthetically designed single guide RNA (sgRNA) used combination various transformation methods or lately available novel nanoparticle-based approaches, allows targeted mutagenesis plants species. Even though this technique is limitless, it still not been employed many species to date. Chickpea second most crucial winter grain crop cultivated worldwide; there currently no reports on gene chickpea. Here, we selected

Language: Английский

Citations

136

Review on factors affecting and control of post-acidification in yoghurt and related products DOI
Gaurav Kr Deshwal,

Swati Tiwari,

Ajay Kumar

et al.

Trends in Food Science & Technology, Journal Year: 2021, Volume and Issue: 109, P. 499 - 512

Published: Jan. 30, 2021

Language: Английский

Citations

126

Recent Advances for Drought Stress Tolerance in Maize (Zea mays L.): Present Status and Future Prospects DOI Creative Commons
Seema Sheoran,

Yashmeet Kaur,

Sushil Kumar

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: May 30, 2022

Drought stress has severely hampered maize production, affecting the livelihood and economics of millions people worldwide. In future, as a result climate change, unpredictable weather events will become more frequent hence implementation adaptive strategies be inevitable. Through utilizing different genetic breeding approaches, efforts are in progress to develop drought tolerance maize. The recent approaches genomics-assisted breeding, transcriptomics, proteomics, transgenics, genome editing have fast-tracked enhancement for under laboratory field conditions. could considerably improved by combining omics technologies with novel methods high-throughput phenotyping (HTP). This review focuses on responses against drought, well system biology applied better understand mechanisms development drought-tolerant cultivars. Researchers must disentangle molecular physiological bases features order increase yield. Therefore, integrated investments field-based HTP, biology, sophisticated methodologies expected help stabilize production face change.

Language: Английский

Citations

74

Effects of Climate Change and Drought Tolerance on Maize Growth DOI Creative Commons
Kyung‐Hee Kim,

Byung‐Moo Lee

Plants, Journal Year: 2023, Volume and Issue: 12(20), P. 3548 - 3548

Published: Oct. 12, 2023

Climate change is affecting all regions of the world with different climates, and scale damage increasing due to occurrence various natural disasters. In particular, maize production highly affected by abnormal climate events such as heat waves droughts. Increasing temperatures can accelerate growth shorten growing season, potentially reducing productivity. Additionally, enhanced during ripening period process, crop yields. addition, drought stress water deficit greatly affect seedling formation, early plant growth, photosynthesis, reproductive yield, so proper management critical growth. Maize, in tall broad-leaved, extreme at planting cause leaves curl stunt It important understand that severe have a detrimental effect on reproduction maize. high caused inhibit induction flowering male flowers factors interfere pollen development. therefore increase productivity food crops, including maize, while maintaining them face persistent change. This requires strategy develop genetically modified crops drought-tolerant effectively respond The aim this paper investigate effects tolerance We also reviewed molecular breeding techniques varieties response

Language: Английский

Citations

51

Perspectives on sustainable food production system: Characteristics and green technologies DOI Creative Commons
Bai Qu,

Zhenlei Xiao,

Abhinav Upadhyay

et al.

Journal of Agriculture and Food Research, Journal Year: 2024, Volume and Issue: 15, P. 100988 - 100988

Published: Jan. 14, 2024

Sustainable food production covers a wide range of perspectives and embraces issues relating to environment, economy society. Sustainability systems faces challenges ranging from environmental degradation, resource competition elevated demands integration agriculture into the global economy. Considering its far-reaching socio-economic implications, this review first provides an overview key characteristics that distinguish sustainable conventional agricultural practices. In addition, technologies in promoting are summarized. Among those modern techniques, urban agriculture, next-generation plant-based foods nanotechnology discussed detail. Finally, futuristic solutions research work proposed provide guidance for designing Agri-Food system.

Language: Английский

Citations

20

Plant pangenomics: approaches, applications and advancements DOI
Monica F. Danilevicz, Cassandria G. Tay Fernandez, Jacob I. Marsh

et al.

Current Opinion in Plant Biology, Journal Year: 2020, Volume and Issue: 54, P. 18 - 25

Published: Jan. 23, 2020

Language: Английский

Citations

107

Safety Aspects of Genetically Modified Lactic Acid Bacteria DOI Creative Commons

Tina Vida Plavec,

Aleš Berlec

Microorganisms, Journal Year: 2020, Volume and Issue: 8(2), P. 297 - 297

Published: Feb. 21, 2020

Lactic acid bacteria (LAB) have a long history of use in the food industry. Some species are part normal human microbiota and beneficial properties for health. Their long-standing considerable biotechnological potential led to development various systems their engineering. Together with novel approaches such as CRISPR-Cas, established engineering now allow significant improvements LAB strains. Nevertheless, genetically modified (GM-LAB) still encounter disapproval under extensive regulatory requirements. This review presents data on prospects obtain ‘generally recognized safe’ (GRAS) status. Genetic modification is discussed, together problems that can arise from engineering, including dissemination into environment spread antibiotic resistance markers. Possible solutions would GM-LAB described, biocontainment, alternative selection markers, homologous DNA. The cell factories closed prevent environmental release least problematic aspect, this also discussed.

Language: Английский

Citations

99

Mechanistic insights of CRISPR/Cas‐mediated genome editing towards enhancing abiotic stress tolerance in plants DOI
Mujtaba Aamir Bhat, Rakeeb Ahmad Mir, Vijay Kumar

et al.

Physiologia Plantarum, Journal Year: 2021, Volume and Issue: 172(2), P. 1255 - 1268

Published: Feb. 12, 2021

Abstract Abiotic stresses such as temperature (high/low), drought, salinity, and others make the environment hostile to plants. stressors adversely affect plant growth development; thereby makes a direct impact on overall productivity. Plants confront stress by developing an internal defense system orchestrated compatible solutes, reactive oxygen species scavengers phytohormones. However, routine exposure unpredictable environmental it essential equip plants with that contributes sustainable agricultural productivity, besides imparting multi‐stress tolerance. The approach against abiotic is accomplished through breeding of tolerant cultivars. Though eco‐friendly, tedious screening crossing protocol limits its usage overcome in attaining goal global food security. Advancement technological front has enabled adoption genomic engineering approaches perform site‐specific modification genome for improving adaptability, increasing yield attributing resilience different stressors. Of editing approaches, CRISPR/Cas revolutionized biological research wider applicability crop emerged versatile tool genomes desired traits highly accurate precise manner. present study summarizes advancement manipulate novel towards high‐yielding climate‐resilient varieties.

Language: Английский

Citations

94

Blocking intruders: inducible physico-chemical barriers against plant vascular wilt pathogens DOI Creative Commons
Anurag Kashyap, Marc Planas‐Marquès, Montserrat Capellades

et al.

Journal of Experimental Botany, Journal Year: 2020, Volume and Issue: 72(2), P. 184 - 198

Published: Sept. 16, 2020

Xylem vascular wilt pathogens cause devastating diseases in plants. Proliferation of these the xylem causes massive disruption water and mineral transport, resulting severe wilting death infected Upon reaching tissue, multiply profusely, spreading vertically within sap, horizontally between vessels to surrounding tissues. Plant resistance is very complex. One most effective defense responses resistant plants formation physico-chemical barriers tissue. Vertical spread vessel lumen restricted by structural barriers, namely, tyloses gels. Horizontal apoplast healthy tissues prevented coating colonized with lignin suberin. Both vertical horizontal compartmentalize pathogen at infection site contribute their elimination. Induction defenses are tightly coordinated, both temporally spatially, avoid detrimental consequences such as cavitation embolism. We discuss current knowledge on mechanisms underlying plant-inducible against major xylem-colonizing pathogens. This may be applied engineer metabolic pathways compounds specific cells, produce towards colonizers.

Language: Английский

Citations

89