Diverse roles of MYB transcription factors in regulating secondary metabolite biosynthesis, shoot development, and stress responses in tea plants (Camellia sinensis)

The Plant Journal, Journal Year: 2022, Volume and Issue: 110(4), P. 1144 - 1165

Published: March 12, 2022

SUMMARY Tea ( Camellia sinensis ) is concocted from tea plant shoot tips that produce catechins, caffeine, theanine, and terpenoids, which collectively determine the rich flavors health benefits of infusion. However, little known about integrated regulation tip development characteristic secondary metabolite biosynthesis in plants. Here, we demonstrate MYB transcription factors (TFs) play key yet diverse roles regulating leaf stem development, biosynthesis, environmental stress responses By integrating transcriptomic metabolic profiling data different tissues at a series developmental stages or under various conditions, alongside biochemical genetic analyses, predicted TFs involved (CsMYB2, 98, 107, 221), epidermal cell initiation (CsMYB184, 41, 139, 219), stomatal (CsMYB113 153), flavonoids (including anthocyanins, flavonols; CsMYB8 99), caffeine (CsMYB85 86), theanine (CsMYB9 49), carotenoids (CsMYB110), mono‐/sesquiterpenoid volatiles (CsMYB68, 147, 148, 193), lignin (CsMYB164 192), indolic compounds (CsMYB139, 162, 198), as well are likely hormone signaling‐mediated defense responses. We characterized functions some MYBs flavonoid carotenoid for quality flavor. This study provides cross‐family analysis new insights into coordinated metabolism, paving way towards understanding trait formation improvement

Language: Английский

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CsMYB1 integrates the regulation of trichome development and catechins biosynthesis in tea plant domestication

New Phytologist, Journal Year: 2022, Volume and Issue: 234(3), P. 902 - 917

Published: Feb. 15, 2022

Summary Tea trichomes synthesize numerous specialized metabolites to protect plants from environmental stresses and contribute tea flavours, but little is known about the regulation of trichome development. Here, we showed that CsMYB1 involved in formation galloylated cis ‐catechins biosynthesis plants. The variations expression levels are closely correlated with indexes contents plant populations. Genome resequencing may be selected modern cultivars, since a 192‐bp insertion promoter was found exclusively cultivars not glabrous wild Camellia taliensis . Several enhancers increased transcription coincided their higher indexes. Biochemical analyses transgenic data interacted CsGL3 CsWD40 formed MYB‐bHLH‐WD40 (MBW) transcriptional complex activate regulator genes CsGL2 CsCPC , anthocyanidin reductase serine carboxypeptidase‐like 1A integratively regulated biosynthesis. Results suggest coincidently during domestication by harsh environments for improved adaption breeders better flavours.

Language: Английский

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Single‐cell transcriptome atlas reveals developmental trajectories and a novel metabolic pathway of catechin esters in tea leaves

Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 20(11), P. 2089 - 2106

Published: July 10, 2022

The tea plant is an economically important woody beverage crop. unique taste of evoked by certain metabolites, especially catechin esters, whereas their precise formation mechanism in different cell types remains unclear. Here, a fast protoplast isolation method was established and the transcriptional profiles 16 977 single cells from 1st 3rd leaves were investigated. We first identified 79 marker genes based on six isolated tissues constructed transcriptome atlas, mapped developmental trajectories further delineated distribution during leaf differentiation associated with fate transformation. Interestingly, eight differently expressed found to co-exist at four branch points. Genes involved biosynthesis metabolites showed cell- development-specific characteristics. An unexpected ester glycosyltransferase characterized for time plants gene co-expression network mesophyll cells. Thus, single-cell landscape crop leave reported novel metabolism pathway esters discovered.

Language: Английский

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A rapid and efficient transient expression system for gene function and subcellular localization studies in the tea plant (Camellia sinensis) leaves

Scientia Horticulturae, Journal Year: 2022, Volume and Issue: 297, P. 110927 - 110927

Published: Feb. 1, 2022

Language: Английский

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CsMYBL2 homologs modulate the light and temperature stress‐regulated anthocyanin and catechins biosynthesis in tea plants (Camellia sinensis)

The Plant Journal, Journal Year: 2023, Volume and Issue: 115(4), P. 1051 - 1070

Published: May 11, 2023

SUMMARY Anthocyanin and catechin production in tea ( Camellia sinensis ) leaves can positively affect quality; however, their regulatory mechanisms are not fully understood. Here we report that, while the CsMYB75‐ or CsMYB86‐directed MYB–bHLH–WD40 (MBW) complexes differentially activate anthocyanin biosynthesis leaves, respectively, CsMYBL2a CsMYBL2b homologs negatively modified light‐ temperature‐induced both Arabidopsis plants. The MBW activated synthesis genes downstream repressor . Overexpression of , but repressed leaf accumulation seed coat proanthocyanin production. strongly weakly activating effects CsMYB75/CsMYB86 on CsDFR CsANS due to different EAR TLLLFR domains interactions with CsTT8/CsGL3, interfering functions complexes. play roles fine‐tuning CsMYB75/CsMYB86–MBW activation anthocyanins catechins, respectively. CsbZIP1 – CsmiR858a CsMYBL2 module mediated UV‐B‐ cold‐activated regulation anthocyanin/catechin by repressing Similarly, CsCOP1–CsbZIP1–CsPIF3 module, BR signaling as well, high temperature repression through upregulating present study provides new insights into complex networks environmental stress‐modified flavonoid plant leaves.

Language: Английский

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Epigallocatechin-3-Gallate (EGCG): A unique secondary metabolite with diverse roles in plant-environment interaction

Environmental and Experimental Botany, Journal Year: 2023, Volume and Issue: 209, P. 105299 - 105299

Published: March 17, 2023

Language: Английский

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A CCA1-like MYB subfamily member CsMYB128 participates in chilling sensitivity and cold tolerance in tea plants (Camellia sinensis)

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139473 - 139473

Published: Jan. 1, 2025

Language: Английский

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The regulation of auxin receptor gene CsAFB2 by csn‐miR393a confers resistance against Colletotrichum gloeosporioides in tea plants

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(4)

Published: March 28, 2025

Anthracnose, a severe disease caused by Colletotrichum, affects diverse crops and leads to significant economic losses through pronounced fruit/leaf lesions. MicroRNAs (miRNAs) play crucial roles in modulating gene expression response resistance, defence responses plant immunity. However, the regulatory mechanisms of miRNAs Colletotrichum gloeosporioides remain unknown tea plants. Our study revealed that csn-miR393a targets auxin receptor CsAFB2 during resistance C. plants comparing resistant cultivar Zhongcha108 susceptible Longjing43. Through Nicotiana benthamiana leaf co-transformation assays, we demonstrated suppresses CsAFB2, target mimic blocks function csn-miR393a, leading increase CsAFB2. Repression transcripts leaves antisense oligonucleotides negatively regulating reactive oxygen species homoeostasis, PR catechin accumulation. To further validate developed transgenic overexpressing resulting enhanced against gloeosporioides. Additionally, N. lines provided evidence regulates suppressing Therefore, manipulating or its gene, has potential strengthen plant's anthracnose.

Language: Английский

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Dissection of the spatial dynamics of biosynthesis, transport, and turnover of major amino acids in tea plants (Camellia sinensis)

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(5)

Published: Feb. 19, 2024

Abstract High levels of free amino acids (AAs) in tea leaves are crucial for flavor and health function; however, the dynamic AA biosynthesis, transport, turnover plants remain elusive. Here we dissected whole these dynamics by assessing profiles transcriptomes metabolic pathway genes roots, stems, revealing their distinctive features with regard to synthesis, degradation/recycling. Nitrogen assimilation dominated roots wherein glutamine (Gln), theanine, arginine (Arg) were actively synthesized. Arg was transported into trunk together Glu, Gln, theanine as major AAs xylem sap long-distance root-to-leaf transport. Transcriptome analysis revealed that involved synthesis highly expressed but those transport degradation stems young leaves, respectively. CsGSIa transcripts found root meristem cells, root, stem leaf vascular tissues, mesophyll where it appeared participate recycling. Overexpression transgenic hairy knockdown produced higher lower Gln than wild-type This study provides comprehensive new insights metabolism plant.

Language: Английский

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Metabolite Profiling and Transcriptome Analysis Revealed the Conserved Transcriptional Regulation Mechanism of Caffeine Biosynthesis in Tea and Coffee Plants

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(10), P. 3239 - 3251

Published: March 4, 2022

Caffeine is a characteristic bioactive compound in tea and coffee plants, which synthesized accumulated extensively leaves seeds. However, little known about the regulatory mechanism of caffeine synthesis plants. This study compared metabolite between We found that contained significantly higher than leaves, perhaps due to more members N-methyltransferase (NMT) genes as well expression levels Substantial numbers transcription factors were predicted be involved biosynthesis regulation, combining weighted gene co-expression network analysis cis-element NMT promoter Furthermore, from caffeine-related modules suggested was probably partly conservative provides an essential resource for

Language: Английский

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