Identification of Dopaminergic Neurons That Can Both Establish Associative Memory and Acutely Terminate Its Behavioral Expression DOI Open Access
Michael Schleyer, Aliće Weiglein, Juliane Thoener

et al.

Journal of Neuroscience, Journal Year: 2020, Volume and Issue: 40(31), P. 5990 - 6006

Published: June 25, 2020

An adaptive transition from exploring the environment in search of vital resources to exploiting these once was successful is important all animals. Here we study neuronal circuitry that allows larval Drosophila melanogaster either sex negotiate this exploration-exploitation transition. We do so by combining Pavlovian conditioning with high-resolution behavioral tracking, optogenetic manipulation individually identified neurons, and EM data-based analyses synaptic organization. find activation dopaminergic neuron DAN-i1 can both establish memory during training acutely terminate learned behavior a subsequent recall test. Its leaves innate unaffected, however. Specifically, associative memories opposite valence after paired unpaired odor, its test resulting memories. Our results further suggest significance resembles, but does not equal, sugar reward. Dendrogram connections between two main targets, Kenyon cells mushroom body output MBON-i1, signals could be delivered respectively, within previously unrecognized, locally confined branching structures. This would provide an elegant circuit motif on completion. SIGNIFICANCE STATEMENT In struggle for survival, animals have explore their food. Once food found, however, it prioritize over continuing now as pointless searching glasses you are wearing. trade-off humans, well technical devices. investigate which only 10,000 neurons fruit fly larva tip balance trade-off, identify single dopamine called so. Given similarities function across animal kingdom, may reflect general principle how terminated successful.

Language: Английский

The connectome of the adult Drosophila mushroom body provides insights into function DOI Creative Commons
Feng Li, Jack Lindsey, Elizabeth C. Marin

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Language: Английский

Citations

349
Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior DOI
Dick R. Nässel, Meet Zandawala

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

Citations

311
The natverse, a versatile toolbox for combining and analysing neuroanatomical data DOI Creative Commons
Alexander Shakeel Bates, James D. Manton, Sridhar R. Jagannathan

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: April 14, 2020

To analyse neuron data at scale, neuroscientists expend substantial effort reading documentation, installing dependencies and moving between analysis visualisation environments. facilitate this, we have developed a suite of interoperable open-source R packages called the natverse. The natverse allows users to read local remote data, perform popular analyses including clustering graph-theoretic neuronal branching. Unlike most tools, enables comparison across many neurons morphology connectivity after imaging or co-registration within common template space. also transformations different spaces modalities. We demonstrate tools that integrate vast majority

Language: Английский

Citations

207
Recurrent architecture for adaptive regulation of learning in the insect brain DOI
Claire Eschbach, Akira Fushiki, Michael Winding

et al.

Nature Neuroscience, Journal Year: 2020, Volume and Issue: 23(4), P. 544 - 555

Published: March 23, 2020

Language: Английский

Citations

151
Biological underpinnings for lifelong learning machines DOI
Dhireesha Kudithipudi, Mario Aguilar-Simon,

Jonathan Babb

et al.

Nature Machine Intelligence, Journal Year: 2022, Volume and Issue: 4(3), P. 196 - 210

Published: March 23, 2022

Language: Английский

Citations

143
Whole-body integration of gene expression and single-cell morphology DOI Creative Commons
Hernando Martínez Vergara, Constantin Pape, Kimberly Meechan

et al.

Cell, Journal Year: 2021, Volume and Issue: 184(18), P. 4819 - 4837.e22

Published: Aug. 10, 2021

Animal bodies are composed of cell types with unique expression programs that implement their distinct locations, shapes, structures, and functions. Based on these properties, assemble into specific tissues organs. To systematically explore the link between cell-type-specific gene morphology, we registered an atlas to a whole-body electron microscopy volume nereid Platynereis dumerilii. Automated segmentation cells nuclei identifies major classes establishes activation, chromatin topography, nuclear size. Clustering segmented according reveals spatially coherent tissues. In brain, genetically defined groups neurons match ganglionic projections. Besides interneurons, uncover sensory-neurosecretory in mushroom bodies, which thus qualify as sensory They furthermore resemble vertebrate telencephalon by molecular anatomy. We provide integrated browser Fiji plugin for remote exploration all available multimodal datasets.

Language: Английский

Citations

105
Connectomics and function of a memory network: the mushroom body of larval Drosophila DOI
Andreas S. Thum, Bertram Gerber

Current Opinion in Neurobiology, Journal Year: 2018, Volume and Issue: 54, P. 146 - 154

Published: Oct. 24, 2018

Language: Английский

Citations

87
Can insects feel pain? A review of the neural and behavioural evidence DOI
Matilda Gibbons, Andrew Crump, Meghan Barrett

et al.

Advances in insect physiology, Journal Year: 2022, Volume and Issue: unknown, P. 155 - 229

Published: Jan. 1, 2022

Language: Английский

Citations

64
Learning and memory using Drosophila melanogaster: a focus on advances made in the fifth decade of research DOI Creative Commons
Ronald L. Davis

Genetics, Journal Year: 2023, Volume and Issue: 224(4)

Published: May 22, 2023

Abstract In the last decade, researchers using Drosophila melanogaster have made extraordinary progress in uncovering mysteries underlying learning and memory. This has been propelled by amazing toolkit available that affords combined behavioral, molecular, electrophysiological, systems neuroscience approaches. The arduous reconstruction of electron microscopic images resulted a first-generation connectome adult larval brain, revealing complex structural interconnections between memory-related neurons. serves as substrate for future investigations on these connections building complete circuits from sensory cue detection to changes motor behavior. Mushroom body output neurons (MBOn) were discovered, which individually forward information discrete non-overlapping compartments axons mushroom (MBn). These mirror previously discovered tiling inputs dopamine led model ascribes valence event, either appetitive or aversive, activity different populations balance MBOn promoting avoidance approach Studies calyx, houses MBn dendrites, revealed beautiful microglomeruluar organization synapses occur with long-term memory (LTM) formation. Larval advanced, positioning it possibly lead producing new conceptual insights due its markedly simpler structure over brain. Advances how cAMP response element-binding protein interacts kinases other transcription factors promote formation LTM. New Orb2, prion-like forms oligomers enhance synaptic synthesis required LTM Finally, research pioneered our understanding mechanisms mediate permanent transient active forgetting, an important function brain along acquisition, consolidation, retrieval. was catalyzed partly identification suppressor genes—genes whose normal is limit

Language: Английский

Citations

39
Metamorphosis of memory circuits in Drosophila reveals a strategy for evolving a larval brain DOI Creative Commons
James W. Truman,

Jacquelyn Price,

Rosa Linda Miyares

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 25, 2023

Mushroom bodies (MB) of adult Drosophila have a core thousands Kenyon neurons; axons the early-born g class form medial lobe and those from later-born α'β' αβ classes both vertical lobes. The larva, however, hatches with only γ neurons forms 'facsimile' using larval-specific axon branches its neurons. MB input (MBINs) output (MBONs) divide neuron lobes into discrete computational compartments. larva has 10 such compartments while 16. We determined fates 28 32 MBONs MBINs that define larval Seven are subsequently incorporated MB; four their die, 12 MBINs/MBONs remodel to function in remaining three specific. At metamorphosis MBIN/MBONs trans-differentiate, leaving for other brain circuits. made de novo MBONs/MBINs recruited pools adult-specific combination cell death, compartment shifting, trans-differentiation, recruitment new result no MBIN-MBON connections being maintained through metamorphosis. this simple level, then, we find anatomical substrate memory trace persisting adult. phenotype trans-differentiating represents evolutionarily ancestral is derived adaptation stage. These cells arise primarily within lineages also produce permanent MBONs, suggesting specifying factors may allow information related birth-order or sibling identity be interpreted modified manner these acquire phenotypic modifications. loss at then allows revert functions

Language: Английский

Citations

38