Cellular Imaging Using Stimulated Raman Scattering Microscopy DOI
Andrew H. Hill, Dan Fu

Analytical Chemistry, Journal Year: 2019, Volume and Issue: 91(15), P. 9333 - 9342

Published: June 25, 2019

Cellular imaging is an active area of research that enables researchers to monitor cellular dynamics, as well responses various external stimuli (physiological stress, exogenous compounds, etc.). Stimulated Raman scattering (SRS) microscopy one popular experimental tool used image cells, largely because its chemical specificity, high spatial resolution, and acquisition speed. In this Perspective, the theoretical background implementation SRS are discussed recent developments in field with highlighted summarized.

Language: Английский

Denoising of stimulated Raman scattering microscopy images via deep learning DOI Creative Commons
Bryce Manifold,

Elena C. Thomas,

Andrew T. Francis

et al.

Biomedical Optics Express, Journal Year: 2019, Volume and Issue: 10(8), P. 3860 - 3860

Published: July 10, 2019

Stimulated Raman scattering (SRS) microscopy is a label-free quantitative chemical imaging technique that has demonstrated great utility in biomedical applications ranging from real-time stain-free histopathology to live animal imaging. However, similar many other nonlinear optical techniques, SRS images often suffer low signal noise ratio (SNR) due absorption and of light tissue as well the limitation applicable power minimize photodamage. We present use deep learning algorithm significantly improve SNR images. Our based on U-Net convolutional neural network (CNN) outperforms existing denoising algorithms. More importantly, we demonstrate trained acquired at different zoom, power, depth, geometries are not included training. results identify powerful tool for large, with potential towards vivo applications, where parameters variable ground-truth available create fully supervised training set.

Language: Английский

Citations

109
Mid-infrared metabolic imaging with vibrational probes DOI
Lixue Shi, Xinwen Liu, Lingyan Shi

et al.

Nature Methods, Journal Year: 2020, Volume and Issue: 17(8), P. 844 - 851

Published: June 29, 2020

Language: Английский

Citations

93
Rapid Determination of Antimicrobial Susceptibility by Stimulated Raman Scattering Imaging of D 2 O Metabolic Incorporation in a Single Bacterium DOI Creative Commons
Meng Zhang, Weili Hong, Nader S. Abutaleb

et al.

Advanced Science, Journal Year: 2020, Volume and Issue: 7(19)

Published: Aug. 16, 2020

Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with appropriate antibiotics and slowing down the emergence of antibiotic-resistant bacteria. Here, a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging deuterium oxide (D2 O) metabolism reported. Metabolic incorporation D2 O into biomass in single bacterium metabolic response to are probed as short 10 min after culture 70% medium, fastest among current technologies. Single-cell inactivation concentration (SC-MIC) obtained less than 2.5 h from colony results. The SC-MIC 37 sets bacterial isolate samples, which include 8 major species 14 different often encountered clinic, validated standard minimal inhibitory blindly measured via broth microdilution. Toward clinical translation, determination 1 antibiotic treatment 30 mixture incubation bacteria urine or whole blood demonstrated.

Language: Английский

Citations

91
Portable all-fiber dual-output widely tunable light source for coherent Raman imaging DOI Creative Commons

Maximilian Brinkmann,

Alexander Fast,

Tim Hellwig

et al.

Biomedical Optics Express, Journal Year: 2019, Volume and Issue: 10(9), P. 4437 - 4437

Published: Aug. 5, 2019

We present a rapidly tunable dual-output all-fiber light source for coherent Raman imaging, based on dispersively matched mode-locked laser pumping parametric oscillator. Output pump and Stokes pulses with maximal power of 170 400 mW, respectively, equal durations 7 ps could be generated. The tuning mechanism required no mechanical delay line, enabling all-electronic arbitrary wavelength switching across more than 2700 cm-1 in less 5 ms. compact setup showed reliable operation despite shocks 25 m/s2 is, thus, well suited mobile cart. Imaging mouse human skin tissue both the portable commercial laboratory-bound reference system yielded qualitatively results verified being microscopy.

Language: Английский

Citations

83
Raman Spectroscopy and Imaging in Bioanalytics DOI Creative Commons
Dana Cialla‐May, Christoph Krafft, Petra Rösch

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 94(1), P. 86 - 119

Published: Dec. 17, 2021

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTRaman Spectroscopy and Imaging in BioanalyticsDana Cialla-MayDana Cialla-MayLeibniz-Institute of Photonic Technology, Member the Leibniz Research Alliance − Health Technologies, Albert-Einstein-Str. 9, 07745 Jena, GermanyInstitute Physical Chemistry Abbe Center Photonics, Friedrich Schiller University, Helmholtzweg 4, 07743 GermanyInfectoGnostics Campus Applied Research, Philosophenweg 7, GermanyMore by Dana Cialla-MayView Biography, Christoph KrafftChristoph KrafftLeibniz-Institute KrafftView Biographyhttps://orcid.org/0000-0003-1049-0560, Petra RöschPetra RöschInstitute RöschView Biographyhttps://orcid.org/0000-0001-6179-3719, Tanja Deckert-GaudigTanja Deckert-GaudigLeibniz-Institute Deckert-GaudigView Torsten FroschTorsten FroschLeibniz-Institute FroschView Izabella J. JahnIzabella JahnLeibniz-Institute JahnView Biographyhttps://orcid.org/0000-0002-1186-0925, Susanne PahlowSusanne PahlowLeibniz-Institute PahlowView Clara StiebingClara StiebingLeibniz-Institute StiebingView Tobias Meyer-ZedlerTobias Meyer-ZedlerLeibniz-Institute Meyer-ZedlerView Thomas BocklitzThomas BocklitzLeibniz-Institute BocklitzView Biographyhttps://orcid.org/0000-0003-2778-6624, Iwan SchieIwan SchieLeibniz-Institute GermanyErnst-Abbe-Hochschule University Sciences, Department Biomedical Engineering Biotechnology, Carl-Zeiss-Promenade 2, SchieView Biographyhttps://orcid.org/0000-0003-0336-3168, Volker DeckertVolker DeckertLeibniz-Institute DeckertView Biographyhttps://orcid.org/0000-0002-0173-7974, Jürgen Popp*Jürgen PoppLeibniz-Institute Germany*Email: [email protected]More PoppView Biographyhttps://orcid.org/0000-0003-4257-593XCite this: Anal. Chem. 2022, 94, 1, 86–119Publication Date (Web):December 17, 2021Publication History Published online17 December 2021Published inissue 11 January 2022https://pubs.acs.org/doi/10.1021/acs.analchem.1c03235https://doi.org/10.1021/acs.analchem.1c03235review-articleACS PublicationsCopyright © 2021 The Authors. American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0. License Summary*You are free to share (copy redistribute) this article any medium or format within parameters below:Creative Commons (CC): a Creative license.Attribution (BY): Credit must be given creator.Non-Commercial (NC): Only non-commercial uses work permitted. No Derivatives (ND): Derivative works may created for purposes, but sharing prohibited. View full license*DisclaimerThis summary highlights only some key features terms actual license. It not license has no legal value. Carefully review before using these materials. Open Access indicated. Learn MoreArticle Views8741Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum text downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (12 MB) Get e-AlertscloseSUBJECTS:Anatomy,Fibers,Lasers,Molecules,Raman spectroscopy e-Alerts

Language: Английский

Citations

82
Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities DOI Creative Commons
H. Fujioka, Jingwen Shou, Ryosuke Kojima

et al.

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(49), P. 20701 - 20707

Published: Nov. 23, 2020

Raman probes based on alkyne or nitrile tags hold promise for highly multiplexed imaging. However, sensing of enzyme activities with is difficult because few mechanisms are available to modulate the vibrational response. Here we present a general strategy prepare activatable that show enhanced signals due electronic preresonance (EPR) upon reaction enzymes under physiological conditions. We identified xanthene derivative bearing group at position 9 (9CN-JCP) as suitable scaffold dye, and synthesized four types probes, which targeted different (three aminopeptidases glycosidase) tuned frequencies by isotope editing group. validated activation these target succeeded in simultaneous imaging live cells. Different cell lines showed patterns activities.

Language: Английский

Citations

81
Current Advancements in Transdermal Biosensing and Targeted Drug Delivery DOI Creative Commons
P.C. Pándey,

Shubhangi Shukla,

Shelby A. Skoog

et al.

Sensors, Journal Year: 2019, Volume and Issue: 19(5), P. 1028 - 1028

Published: Feb. 28, 2019

In this manuscript, recent advancements in the area of minimally-invasive transdermal biosensing and drug delivery are reviewed. The administration therapeutic entities through skin is complicated by stratum corneum layer, which serves as a barrier to entry retards bioavailability. A variety strategies have been adopted for enhancement permeation various substances. Physical techniques such iontophoresis, reverse electroporation, microneedles offer (a) electrical amplification sensing biomolecules (b) transport amphiphilic molecules targeted site minimally invasive manner. Iontophoretic involves application low currents well migration polarized neutral across it. Transdermal via has emerged novel approach replace hypodermic needles. addition, facilitated detection analytes body fluids. This review considers innovations structure performance systems.

Language: Английский

Citations

79
Multiplexed live-cell profiling with Raman probes DOI Creative Commons
Chen Chen, Zhilun Zhao, Naixin Qian

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: June 7, 2021

Abstract Single-cell multiparameter measurement has been increasingly recognized as a key technology toward systematic understandings of complex molecular and cellular functions in biological systems. Despite extensive efforts analytical techniques, it is still generally challenging for existing methods to decipher large number phenotypes single living cell. Herein we devise multiplexed Raman probe panel with sharp mutually resolvable peaks simultaneously quantify cell surface proteins, endocytosis activities, metabolic dynamics an individual live When coupling whole-cell spontaneous micro-spectroscopy, demonstrate the utility this technique 14-plexed live-cell profiling phenotyping under various drug perturbations. In particular, single-cell enables powerful clustering, correlation, network analysis insights. This platform compatible cytometry, low instrument complexity capable highly robust straightforward manner, thereby contributing valuable tool both basic biology translation applications such high-content sorting discovery.

Language: Английский

Citations

70
Switchable stimulated Raman scattering microscopy with photochromic vibrational probes DOI Creative Commons
Jianpeng Ao, Xiaofeng Fang, Xianchong Miao

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: May 25, 2021

Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman stimulated scattering (SRS) microscopy provides superb resolution for super-multiplexed imaging, are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow peak group shifts reversibly upon photoisomerization conjugated when irradiated ultraviolet (UV) or visible light, yielding "on" "off" images taken at photoactive frequency. We demonstrated photo-rewritable patterning encryption on thin films, painting/erasing cells labelled alkyne-diarylethene, as well pulse-chase experiments mitochondria diffusion in living cells. design principle potentials optical memories switches

Language: Английский

Citations

59
Analyzing macromolecular composition of E. Coli O157:H7 using Raman-stable isotope probing DOI Creative Commons
Heera Jayan, Hongbin Pu, Da‐Wen Sun

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2022, Volume and Issue: 276, P. 121217 - 121217

Published: April 1, 2022

Metabolic dynamics of bacterial cells is needed for understanding the correlation between changes in environmental conditions and cell metabolic activity. In this study, Raman spectroscopy combined with deuterium labelling was used to analyze activity a single Escherichia coli O157:H7 cell. The incorporation from heavy water into cellular biomolecules resulted formation carbon-deuterium (CD) peaks spectra, indicating broad vibrational corresponding CD CH encompassed different specific shifts macromolecules such as protein, lipids, nucleic acid. utilization tryptophan oleic acid by sole carbon source led lipid composition, indicated new second derivative spectra. Thus, proposed method could semi-quantitatively determine total activity, macromolecule identification, protein metabolism

Language: Английский

Citations

52