Breast cancer heterogeneity and its implication in personalized precision therapy

Experimental Hematology and Oncology, Journal Year: 2023, Volume and Issue: 12(1)

Published: Jan. 9, 2023

Abstract Breast cancer heterogeneity determines progression, treatment effects, and prognosis. However, the precise mechanism for this remains unknown owing to its complexity. Here, we summarize origins of breast influence on disease recurrence, therapeutic resistance. We review possible mechanisms research methods used analyze it. also highlight importance cell interactions heterogeneity, which can be further categorized into cooperative competitive interactions. Finally, provide new insights individual treatments based heterogeneity.

Language: Английский

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Fluidic Multivalent Membrane Nanointerface Enables Synergetic Enrichment of Circulating Tumor Cells with High Efficiency and Viability

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(10), P. 4800 - 4806

Published: Feb. 12, 2020

The ubiquitous biomembrane interface, with its dynamic lateral fluidity, allows membrane-bound components to rearrange and localize for high-affinity multivalent ligand-receptor interactions in diverse life activities. Inspired by this, we herein engineered a fluidic nanointerface decorating microfluidic chip aptamer-functionalized leukocyte membrane nanovesicles high-performance isolation of circulating tumor cells (CTCs). This biomimetic active recruitment-binding afforded significant affinity enhancement 4 orders magnitude, exhibiting 7-fold higher capture efficiency compared monovalent aptamer functionalized-chip blood. Meanwhile, this soft inherited the biological benefits natural biomembrane, minimizing background blood cell adsorption maintaining excellent CTC viability (97.6%). Using chip, CTCs were successfully detected all cancer patient samples tested (17/17), suggesting high potential fluidity-enhanced binding strategy clinical applications. We expect bioengineered interface will lead design innovative platforms biomedical field leveraging cell-cell interaction biomaterial.

Language: Английский

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Circulating tumor cells in precision medicine: challenges and opportunities

Trends in Pharmacological Sciences, Journal Year: 2022, Volume and Issue: 43(5), P. 378 - 391

Published: March 7, 2022

Language: Английский

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Update on Epithelial-Mesenchymal Plasticity in Cancer Progression

Annual Review of Pathology Mechanisms of Disease, Journal Year: 2023, Volume and Issue: 19(1), P. 133 - 156

Published: Sept. 27, 2023

Epithelial-mesenchymal transition (EMT) is a cellular process by which epithelial cells lose their characteristics and acquire mesenchymal traits to promote cell movement. This program aberrantly activated in human cancers endows tumor with increased abilities initiation, migration, invasion, metastasis, therapy resistance. The EMT tumors rarely binary often leads series of gradual or intermediate epithelial-mesenchymal states. Functionally, plasticity (EMP) improves the fitness cancer during progression response therapies. Here, we discuss most recent advances our understanding diverse roles EMP progression, resistance address major clinical challenges due EMP-driven phenotypic heterogeneity cancer. Uncovering novel molecular markers key regulators will aid development new therapeutic strategies prevent recurrence overcome

Language: Английский

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The Transcriptional and Epigenetic Landscape of Cancer Cell Lineage Plasticity

Cancer Discovery, Journal Year: 2023, Volume and Issue: 13(8), P. 1771 - 1788

Published: July 20, 2023

Abstract Lineage plasticity, a process whereby cells change their phenotype to take on different molecular and/or histologic identity, is key driver of cancer progression and therapy resistance. Although underlying genetic changes within the tumor can enhance lineage it predominantly dynamic controlled by transcriptional epigenetic dysregulation. This review explores regulators plasticity interplay with other features malignancy, such as dysregulated metabolism, microenvironment, immune evasion. We also discuss strategies for detection treatment highly plastic tumors. Significance: hallmark critical facilitator oncogenic metastasis, resistance, It essential that mechanisms are elucidated enable development effectively target this phenomenon. In review, we describe cell in highlighting therapeutic approaches may be harnessed patient benefit.

Language: Английский

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Recent Advances in Methods for Circulating Tumor Cell Detection

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3902 - 3902

Published: Feb. 15, 2023

Circulating tumor cells (CTCs) are released from primary tumors and transported through the body via blood or lymphatic vessels before settling to form micrometastases under suitable conditions. Accordingly, several studies have identified CTCs as a negative prognostic factor for survival in many types of cancer. also reflect current heterogeneity genetic biological state tumors; so, their study can provide valuable insights into progression, cell senescence, cancer dormancy. Diverse methods with differing specificity, utility, costs, sensitivity been developed isolating characterizing CTCs. Additionally, novel techniques potential overcome limitations existing ones being developed. This literature review describes emerging enriching, detecting, isolating,

Language: Английский

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Digital microfluidic isolation of single cells for -Omics

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Nov. 11, 2020

Abstract We introduce Digital microfluidic Isolation of Single Cells for -Omics (DISCO), a platform that allows users to select particular cells interest from limited initial sample size and connects single-cell sequencing data their immunofluorescence-based phenotypes. Specifically, DISCO combines digital microfluidics, laser cell lysis, artificial intelligence-driven image processing collect the contents single heterogeneous populations, followed by analysis genomes transcriptomes next-generation sequencing, proteomes nanoflow liquid chromatography tandem mass spectrometry. The results described herein confirm utility at levels are equivalent or enhanced relative state art, capable identifying features level nucleotide variations. unique selectivity, context, accountability suggest potential deep any rare population with contextual dependencies.

Language: Английский

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Engineered models to parse apart the metastatic cascade

npj Precision Oncology, Journal Year: 2019, Volume and Issue: 3(1)

Published: Aug. 21, 2019

Abstract While considerable progress has been made in studying genetic and cellular aspects of metastasis with vitro cell culture vivo animal models, the driving mechanisms each step are still relatively unclear due to their complexity. Moreover, little understanding how fitness one metastatic cascade correlates ability survive other subsequent steps. Engineered models incorporate tools such as tailored biomaterials microfabrication mimic human disease progression, which when coupled advanced quantification methods permit comparisons patient samples studies. Here, we review novel techniques that have recently developed dissect key features using primary highly representative microenvironments for purposes advancing personalized medicine precision oncology. Although improvements needed increase tractability accessibility while faithfully simulating microenvironment, these powerful experimental platforms cancer biology, furthering drug screening, facilitating development therapeutics.

Language: Английский

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Microfluidic Single‐Cell Omics Analysis

Small, Journal Year: 2019, Volume and Issue: 16(9)

Published: Sept. 23, 2019

Abstract The commonly existing cellular heterogeneity plays a critical role in biological processes such as embryonic development, cell differentiation, and disease progress. Single‐cell omics‐based heterogeneous studies have great significance for identifying different populations, discovering new types, revealing informative features, uncovering significant interrelationships between cells. Recently, microfluidics has evolved to be powerful technology single‐cell omics analysis due its merits of throughput, sensitivity, accuracy. Herein, the recent advances microfluidic analysis, including platform designs, lysis strategies, techniques, are reviewed. Representative applications complex then summarized. Finally, few perspectives on future challenges development trends microfluidic‐assisted discussed.

Language: Английский

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Epithelial‐to‐mesenchymal‐like transition events in melanoma

FEBS Journal, Journal Year: 2021, Volume and Issue: 289(5), P. 1352 - 1368

Published: May 17, 2021

Epithelial‐to‐mesenchymal transition (EMT), a process through which epithelial tumor cells acquire mesenchymal phenotypic properties, contributes to both metastatic dissemination and therapy resistance in cancer. Accumulating evidence indicates that nonepithelial tumors, including melanoma, can also gain mesenchymal‐like properties increase their propensity decrease sensitivity therapy. In this review, we discuss recent findings, illustrating the striking similarities—but knowledge gaps—between biology of state(s) melanoma from cancers. Based on comparative analysis, suggest hypothesis‐driven experimental approaches further deepen our understanding EMT‐like how such investigations may pave way towards identification clinically relevant biomarkers for prognosis new therapeutic strategies.

Language: Английский

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