Mechanical Stimulation-Induced Calcium Signaling by Piezo1 Channel Activation in Human Odontoblast Reduces Dentin Mineralization DOI Creative Commons
Mayumi Matsunaga,

Maki Kimura,

Takehito Ouchi

et al.

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 24, 2021

Odontoblasts play critical roles in dentin formation and sensory transduction following stimuli on the surface. Exogenous to surface elicit dentinal sensitivity through movement of fluids tubules, resulting cellular deformation. Recently, Piezo1 channels have been implicated mechanosensitive processes, as well Ca 2+ signals odontoblasts. However, human odontoblasts, responses induced by mechanical stimulation, channel expression, its pharmacological properties remain unclear. In present study, we examined functional expression recording direct stimulation-induced signaling matrix protein 1 (DMP-1)-, nestin-, sialophosphoprotein (DSPP)-immunopositive Mechanical stimulation odontoblasts transiently increased intracellular free calcium concentration ([Ca ] i ). Application repeated resulted transient [Ca increases, but did not show any desensitizing effects increases. We also observed a increase neighboring stimulated cells during showing decrease with an increasing distance from mechanically cells. Yoda1 . This was inhibited application Gd 3+ Dooku1, respectively. or Dooku1. When were knocked down gene silencing short hairpin RNA (shRNA), almost completely abolished. knockdown attenuated number Piezo1-immunopositive immunofluorescence analysis, while no Piezo2-immunopositive Alizarin red staining distinctly showed that activation significantly suppressed mineralization, shRNA-mediated enhanced mineralization. These results suggest predominantly activates via opening, rather than Piezo2 channels, signal establishes intercellular odontoblast-odontoblast communication. addition, participates reduction dentinogenesis. Thus, pathway mediated could contribute function two ways: (1) generating (2) suppressing physiological/reactional dentinogenesis, deformation hydrodynamic forces inside tubules.

Language: Английский

SARS-CoV-2 infection of the oral cavity and saliva DOI Creative Commons
Ni Huang, Paola Pérez, Takafumi Kato

et al.

Nature Medicine, Journal Year: 2021, Volume and Issue: 27(5), P. 892 - 903

Published: March 25, 2021

Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema macules—the involvement the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated analyzed two single-cell RNA sequencing datasets human minor salivary glands gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated normalization annotation, classified 34 unique subpopulations between gingiva. Severe acute respiratory syndrome 2 (SARS-CoV-2) viral entry factors ACE2 TMPRSS members were broadly enriched epithelial cells mucosae. orthogonal protein expression assessments, confirmed SARS-CoV-2 infection Saliva from SARS-CoV-2-infected individuals harbored exhibiting sustained infection. Acellular cellular fractions asymptomatic found to transmit ex vivo. Matched nasopharyngeal saliva samples displayed distinct shedding dynamics, burden correlated with COVID-19 symptoms, including loss. Upon recovery, this cohort exhibited IgG antibodies against SARS-CoV-2. Collectively, these data show that an important site for implicate a potential route transmission. Single-cell transcriptomics analyses gingiva, along detection infectious virus virus-specific individuals, support role pathogenesis.

Language: Английский

Citations

705
DLP printed hDPSC-loaded GelMA microsphere regenerates dental pulp and repairs spinal cord DOI
Ying Qian, Jiaxing Gong, Kejie Lu

et al.

Biomaterials, Journal Year: 2023, Volume and Issue: 299, P. 122137 - 122137

Published: April 27, 2023

Language: Английский

Citations

45
A single-cell atlas of human teeth DOI Creative Commons
Pierfrancesco Pagella, Laura De Vargas Roditi, Bernd Stadlinger

et al.

iScience, Journal Year: 2021, Volume and Issue: 24(5), P. 102405 - 102405

Published: April 23, 2021

Teeth exert fundamental functions related to mastication and speech. Despite their great biomedical importance, an overall picture of cellular molecular composition is still missing. In this study, we have mapped the transcriptional landscape various cell populations that compose human teeth at single-cell resolution, analyzed in deeper detail stem microenvironment. Our study identified heterogeneity dental pulp periodontium. Unexpectedly, found signatures were very similar, while respective microenvironments strongly diverged. findings suggest microenvironmental specificity a potential source for functional differences between highly similar cells located tooth compartments open new perspectives toward cell-based therapeutic approaches.

Language: Английский

Citations

86
Dental-Derived Mesenchymal Stem Cells: State of the Art DOI Creative Commons
Bo Li, Takehito Ouchi, Yubin Cao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: June 22, 2021

Mesenchymal stem cells (MSCs) could be identified in mammalian teeth. Currently, dental-derived MSCs (DMSCs) has become a collective term for all the isolated from dental pulp, periodontal ligament, follicle, apical papilla, and even gingiva. These DMSCs possess similar multipotent potential as bone marrow-derived MSCs, including differentiation into that have characteristics of odontoblasts, cementoblasts, osteoblasts, chondrocytes, myocytes, epithelial cells, neural hepatocytes, adipocytes. Besides, also powerful immunomodulatory functions, which enable them to orchestrate surrounding immune microenvironment. properties promising approach injury repair, tissue regeneration, treatment various diseases. This review outlines most recent advances DMSCs’ functions applications enlightens how these are paving path DMSC-based therapies.

Language: Английский

Citations

76
scFates: a scalable python package for advanced pseudotime and bifurcation analysis from single-cell data DOI Creative Commons
Louis Faure, Ruslan Soldatov, Peter V. Kharchenko

et al.

Bioinformatics, Journal Year: 2022, Volume and Issue: 39(1)

Published: Nov. 16, 2022

scFates provides an extensive toolset for the analysis of dynamic trajectories comprising tree learning, feature association testing, branch differential expression and with a focus on cell biasing fate splits at level bifurcations. It is meant to be fully integrated into scanpy ecosystem seamless from single-cell data various modalities (e.g. RNA ATAC).scFates released as open-source software under BSD 3-Clause 'New' License available Python Package Index https://pypi.org/project/scFates/. The source code GitHub https://github.com/LouisFaure/scFates/. Code reproduction tutorials published datasets are https://github.com/LouisFaure/scFates_notebooks.Supplementary Bioinformatics online.

Language: Английский

Citations

64
Spatiotemporal single-cell regulatory atlas reveals neural crest lineage diversification and cellular function during tooth morphogenesis DOI Creative Commons
Junjun Jing, Jifan Feng, Yuan Yuan

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 16, 2022

Cranial neural crest cells are an evolutionary innovation of vertebrates for craniofacial development and function, yet the mechanisms that govern cell fate decisions postmigratory cranial remain largely unknown. Using mouse molar as a model, we perform single-cell transcriptome profiling to interrogate diversification cells. We reveal landscape transcriptional heterogeneity define specific cellular domains during progression cell-derived dental lineage diversification, find each domain makes contribution distinct mesenchymal tissues. Furthermore, IGF signaling-mediated cell-cell interaction between highlights pivotal role autonomous regulation mesenchyme. Importantly, cell-type-specific gene regulatory networks in mesenchyme show Foxp4 is indispensable differentiation periodontal ligament. Our atlas provides comprehensive mechanistic insight into process odontogenic populations.

Language: Английский

Citations

58
Insights into skeletal stem cells DOI Creative Commons
Qiwen Li, Ruoshi Xu, Kexin Lei

et al.

Bone Research, Journal Year: 2022, Volume and Issue: 10(1)

Published: Oct. 19, 2022

Abstract The tissue-resident skeletal stem cells (SSCs), which are self-renewal and multipotent, continuously provide (including chondrocytes, bone cells, marrow adipocytes, stromal cells) for the development homeostasis of system. In recent decade, utilizing fluorescence-activated cell sorting, lineage tracing, single-cell sequencing, studies have identified various types SSCs, plotted commitment trajectory, partially revealed their properties under physiological pathological conditions. this review, we retrospect to SSCs identification functional studies. We discuss principles approaches identify bona fide highlighting pioneering findings that plot atlas SSCs. roles progenitors in long bone, craniofacial tissues, periosteum systematically discussed. further focus on disputes challenges SSC research.

Language: Английский

Citations

46
Lepr‐Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1 DOI Creative Commons
Danting Zhang, Weimin Lin, Shuang Jiang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(29)

Published: Aug. 8, 2023

Periodontium supports teeth in a mechanically stimulated tissue environment, where heterogenous stem/progenitor populations contribute to periodontal homeostasis. In this study, Leptin receptor+ (Lepr+) cells are identified as distinct ligament stem cell (PDLSC) population by single-cell RNA sequencing and lineage tracing. These Lepr+ PDLSCs located the peri-vascular niche, possessing multilineage potential contributing repair response injury. Ablation of disrupts Hyper-loading unloading occlusal forces modulate activation. Piezo1 is demonstrated that mediates mechanosensing conditional Piezo1-deficient mice. Meanwhile, Yoda1, selective activator Piezo1, significantly accelerates growth via induction cells. summary, Lepr marks unique multipotent PDLSC vivo, toward homeostasis Piezo1-mediated mechanosensing.

Language: Английский

Citations

32
Single-cell census of human tooth development enables generation of human enamel DOI Creative Commons
Ammar Alghadeer, Sesha Hanson-Drury, Anjali P. Patni

et al.

Developmental Cell, Journal Year: 2023, Volume and Issue: 58(20), P. 2163 - 2180.e9

Published: Aug. 14, 2023

Language: Английский

Citations

32
Mesenchymal condensation in tooth development and regeneration: a focus on translational aspects of organogenesis DOI
Bing‐Dong Sui,

Chenxi Zheng,

Wanmin Zhao

et al.

Physiological Reviews, Journal Year: 2023, Volume and Issue: 103(3), P. 1899 - 1964

Published: Jan. 19, 2023

The teeth are vertebrate-specific, highly specialized organs performing fundamental functions of mastication and speech, the maintenance which is crucial for orofacial homeostasis further linked to systemic health human psychosocial well-being. However, with limited ability self-repair, can often be impaired by traumatic, inflammatory, progressive insults, leading high prevalence tooth loss defects worldwide. Regenerative medicine holds promise achieve physiological restoration lost or damaged organs, in particular an evolving framework developmental engineering has pioneered functional regeneration harnessing odontogenic program. As a key event morphogenesis, mesenchymal condensation dictates dental tissue formation patterning through cellular self-organization signaling interaction epithelium, provides representative decipher organogenetic mechanisms leveraged regenerative purposes. In this review, we summarize how spatiotemporally assembles from stem cells (DSCs) sequentially mediates development. We highlight condensation-mimetic efforts based on ex vivo aggregation DSCs, have achieved functionally robust physiologically relevant after implantation animals humans. discussion aspect will add knowledge development-inspired strategies offer benefits propel clinical organ regeneration.

Language: Английский

Citations

28