The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 743 - 743

Published: March 27, 2024

Mesenchymal stem cells (MSCs) have been recognized as a cell therapy with the potential to promote skin healing. MSCs, their multipotent differentiation ability, can generate various related wound healing, such dermal fibroblasts (DFs), endothelial cells, and keratinocytes. In addition, MSCs neovascularization, cellular regeneration, tissue healing through mechanisms including paracrine autocrine signaling. Due these characteristics, extensively studied in context of burn chronic repair. Furthermore, during investigation unique roles aging scarless also discovered. this review, we summarize by which discuss recent findings from preclinical clinical studies. We explore strategies enhance therapeutic effects MSCs. Moreover, emerging trend combining engineering techniques, leveraging advantages materials, biodegradable scaffolds hydrogels, repair capacity Additionally, highlight using characteristics cell-free therapies future direction cell-based treatments, further demonstrating regenerative aesthetic applications regeneration.

Language: Английский

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Mesenchymal stem cells derived exosomes: a new era in cardiac regeneration

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 23, 2025

Language: Английский

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Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(21), P. 12018 - 12018

Published: Nov. 6, 2021

The therapeutic potential of the dental pulp stem (DSC) cell-derived secretome, consisting various biomolecules, is undergoing intense research. Despite promising in vitro and vivo studies, most DSC secretome-based therapies have not been implemented human medicine because paracrine effect bioactive factors secreted by cells (hDPSCs) exfoliated deciduous teeth (SHEDs) completely understood. In this review, we outline current data on hDPSC- SHED-derived secretome as a candidate regeneration bone, cartilage, nerve tissue. Published reports demonstrate that MSC-derived secretome/conditional medium may be effective treating neurodegenerative diseases, neural injuries, cartilage defects, repairing bone regulating neuroprotective, anti-inflammatory, antiapoptotic, angiogenic processes through mechanisms. Dental MSC-secretomes, similarly to marrow MSC-secretome activate molecular cellular mechanisms, which determine effectiveness cell-free therapy. Many emphasize secretomes application tissue-regenerating therapy due their multidirectional observed many different injured tissues.

Language: Английский

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Melatonin enhances osteogenic differentiation of dental pulp mesenchymal stem cells by regulating MAPK pathways and promotes the efficiency of bone regeneration in calvarial bone defects

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: Feb. 19, 2022

Abstract Background Mesenchymal stem cell (MSC)-based tissue engineering plays a major role in regenerative medicine. However, the efficiency of MSC transplantation and survival engrafted cells remain challenging. Melatonin can regulate biology. its function osteogenic differentiation dental pulp-derived MSCs (DPSCs) remains unclear. We investigated effects mechanisms melatonin on bone regeneration capacities DPSCs. Methods The biological signaling with different concentrations DPSCs were evaluated using proliferation assay, quantitative alkaline phosphatase (ALP) activity, Alizarin red staining, real-time polymerase chain reaction, western blot vitro culture model. vivo assessed among empty control, MBCP, MBCP + DPSCs, preconditioning four-created calvarial defects by micro-computed tomographic, histological, histomorphometric, immunohistochemical analyses after 4 8 weeks healing. Results In experiments revealed that (1, 10, 100 μM) significantly concentration-dependently promoted proliferation, surface marker expression (CD 146), ALP activity extracellular calcium deposition, gene ( p < 0.05). activated protein ALP, OCN, RUNX-2 inhibited COX-2/NF-κB expression. Furthermore, phosphorylation mitogen-activated kinase (MAPK) p38/ERK was increased treated μM melatonin, their inhibitors decreased differentiation. demonstrated implanted bone-grafting materials melatonin-preconditioned exhibited greater volume fraction, trabecular structural modeling, new formation, osteogenesis-related than other three groups at postoperatively Conclusions These results suggest promotes regulating MAPK pathways. Preconditioning before efficiently enhance capacities.

Language: Английский

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Comparative Dental Pulp Stem Cells (DPSCs) and Periodontal Ligament Stem Cells (PDLSCs): Difference in effect of Aspirin on Osteoblast Potential of PDLSCs and DPSCs

Tissue and Cell, Journal Year: 2025, Volume and Issue: 94, P. 102776 - 102776

Published: Feb. 21, 2025

Language: Английский

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Regulation and functional importance of human periodontal ligament mesenchymal stromal cells with various rates of CD146+ cells

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 7, 2025

Introduction Mesenchymal stromal cells (MSCs) with high expression of CD146 have superior properties for tissue regeneration. However, variability in the rate CD146+ among donors is observed. In this study, possible reasons behind human periodontal ligament MSCs (hPDL-MSCs) were explored. Methods hPDL-MSCs isolated from 22 different donors, and rates analyzed by flow cytometry. Furthermore, populations various magnetic separation. The dependency cell proliferation, viability, cycle, osteogenic differentiation on was investigated. Besides, effects factors, like density, confluence, inflammatory environment analyzed. Results exhibited between percentage ranging 3% to 67%. Higher associated higher presumably due S-phase, potential. Prolonged confluence seeding density led decline cells. surface stimulated treatment interleukin-1β tumor necrosis factor-α, inhibited interferon-γ. Conclusion These results suggest that are a promising subpopulation enhancing effectiveness MSC-based regenerative therapies, however affected which must be considered propagation their potential application vivo .

Language: Английский

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Epigallocatechin-3-gallate/mineralization precursors co-delivery hollow mesoporous nanosystem for synergistic manipulation of dentin exposure

Bioactive Materials, Journal Year: 2022, Volume and Issue: 23, P. 394 - 408

Published: Nov. 29, 2022

As a global public health focus, oral plays vital role in facilitating overall health. Defected teeth characterized by exposure of dentin generally increase the risk aggravating diseases. The exposed dentinal tubules provide channels for irritants and bacterial invasion, leading to hypersensitivity even pulp inflammation. Cariogenic adhesion biofilm formation on are responsible tooth demineralization caries. It remains clinical challenge achieve integration tubule occlusion, collagen mineralization, antibiofilm functions managing dentin. To address this issue, an epigallocatechin-3-gallate (EGCG) poly(allylamine)-stabilized amorphous calcium phosphate (PAH-ACP) co-delivery hollow mesoporous silica (HMS) nanosystem (E/PA@HMS) was herein developed. application E/PA@HMS effectively occluded with acid- abrasion-resistant stability inhibited Streptococcus mutans. Intrafibrillar mineralization fibrils remineralization demineralized were induced E/PA@HMS. odontogenic differentiation dental cells high biocompatibility also promoted. Animal experiments showed that durably sealed growth up 14 days. Thus, development provides promising benefits protecting through coordinated manipulation caries hypersensitivity.

Language: Английский

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Adult Mesenchymal Stem Cells from Oral Cavity and Surrounding Areas: Types and Biomedical Applications

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(8), P. 2109 - 2109

Published: Aug. 9, 2023

Adult mesenchymal stem cells are those obtained from the conformation of dental structures (DMSC), such as deciduous and permanent teeth other surrounding tissues. Background: The self-renewal differentiation capacities these adult allow for great clinical potential. Because DMSC ectomesenchymal origin, they reveal a high capacity complete regeneration pulp, periodontal tissue, biomedical applications; their into types promotes repair in muscle cardiac, pancreatic, nervous, bone, cartilage, skin, corneal tissues, among others, with predictability success. Therefore, progenitor cells, exosomes origin areas oral cavity due to plasticity, considered fundamental pillar medicine regenerative dentistry. Tissue engineering (MSCs, scaffolds, bioactive molecules) sustains induces its multipotent immunomodulatory effects. It is vital importance guarantee safety efficacy procedures designed patients, this purpose, more trials needed increase several pathologies. Conclusion: From bioethical transcendental anthropological point view, human person unique being facilitates better personalized therapy, given higher prevalence chronic systemic diseases.

Language: Английский

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Mesenchymal Stromal Cells Derived from Dental Tissues: Immunomodulatory Properties and Clinical Potential

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 1986 - 1986

Published: Feb. 6, 2024

Mesenchymal stem/stromal cells (MSCs) are multipotent located in different areas of the human body. The oral cavity is considered a potential source MSCs because they have been identified several dental tissues (D-MSCs). Clinical trials which from these sources were used shown that effective and safe as treatments for tissue regeneration. Importantly, immunoregulatory capacity has observed all populations; however, this function may vary among types MSCs. Since property clinical interest cell therapy protocols, it relevant to analyze differences capacity, well mechanisms by each type MSC. Interestingly, D-MSCs most suitable regenerating mineralized region. Furthermore, supported due their adequate proliferation, migration, differentiation. There also evidence application protocols against autoimmune diseases other inflammatory conditions immunosuppressive capacity. Therefore, review, at preclinical level combination with found described, addition description applied.

Language: Английский

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Dental Mesenchymal Stem Cell Secretome: An Intriguing Approach for Neuroprotection and Neuroregeneration

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 23(1), P. 456 - 456

Published: Dec. 31, 2021

Mesenchymal stem cells (MSCs) are known for their beneficial effects and regenerative potential. In particular, dental-derived MSCs have the advantage of easier accessibility a non-invasive isolation method. Moreover, thanks to neural crest origin, dental seem more prominent neuroregenerative Indeed, in basal conditions they also express neuronal markers. However, it is now well that actions depend, at least part, on secretome, referring all bioactive molecules released conditioned medium (CM) or extracellular vesicles (EVs). this review we focus applications secretome derived from neuroregeneration neuroprotection. The secretomes different been tested purposes, pulp human exfoliated deciduous teeth most studied. Both CM EVs obtained showed able promote neurite outgrowth neuroprotective effects. Interestingly, MSC stronger compared other sources. For these reasons, may represent promising approach treatments.

Language: Английский

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