Immune cell multiomics analysis reveals contribution of oxidative phosphorylation to B-cell functions and organ damage of lupus DOI
Yusuke Takeshima, Yukiko Iwasaki, M Nakano

et al.

Annals of the Rheumatic Diseases, Journal Year: 2022, Volume and Issue: 81(6), P. 845 - 853

Published: March 2, 2022

Objective Systemic lupus erythematosus (SLE) is the prototypical systemic autoimmune disease. While long-term prognosis has greatly improved, better survival still necessary. The type I interferon (IFN) signature, a prominent feature of SLE, not an ideal therapeutic target or outcome predictor. To explore immunological pathways in SLE more precisely, we performed transcriptomic, epigenomic and genomic analyses using 19 immune cell subsets from peripheral blood. Methods We sorted identified mRNA expression profiles genetic polymorphisms 107 patients with 92 healthy controls. Combined differentially expressed genes quantitative trait loci analysis was conducted to find key driver pathogenesis. Results found epigenetic importance oxidative phosphorylation (OXPHOS)/mitochondrial dysfunction memory B cells. Particularly, OXPHOS-regulating gene, PRDX6 (peroxiredoxin 6), as Prdx6- deficient cells showed upregulated mitochondrial respiration well antibody production. revealed OXPHOS signature associated IFN signalling-related (ISRGs) Furthermore, gene sets related innate signalling among ISRGs presented correlation these two signatures associations organ damage specific clinical phenotypes. Conclusion This work elucidated potential prognostic marker for SLE. Since consists electron transport chain, functional unit mitochondria, findings suggest pathway involved

Language: Английский

Probiotic improves symptomatic and viral clearance in Covid19 outpatients: a randomized, quadruple-blinded, placebo-controlled trial DOI Creative Commons
Pedro Gutiérrez‐Castrellón,

Tania Gandara-Martí,

A.T. Abreu y Abreu

et al.

Gut Microbes, Journal Year: 2022, Volume and Issue: 14(1)

Published: Jan. 11, 2022

Intestinal bacteria may influence lung homeostasis via the gut-lung axis. We conducted a single-center, quadruple-blinded, randomized trial in adult symptomatic Coronavirus Disease 2019 (Covid19) outpatients. Subjects were allocated 1:1 to probiotic formula (strains Lactiplantibacillus plantarum KABP022, KABP023, and KAPB033, plus strain Pediococcus acidilactici KABP021, totaling 2 × 109 colony-forming units (CFU)) or placebo, for 30 days. Co-primary endpoints included: i) proportion of patients complete viral remission; ii) progressing moderate severe disease with hospitalization, death; iii) days on Intensive Care Unit (ICU). Three hundred subjects (median age 37.0 years [range 18 60], 161 [53.7%] women, 126 [42.0%] having known metabolic risk factors), 293 completed study (97.7%). Complete remission was achieved by 78 147 (53.1%) group compared 41 146 (28.1%) placebo (RR: 1.89 [95 CI 1.40-2.55]; P < .001), significant after multiplicity correction. No hospitalizations deaths occurred during study, precluding assessment remaining co-primary outcomes. Probiotic supplementation well-tolerated reduced nasopharyngeal load, infiltrates duration both digestive non-digestive symptoms, placebo. compositional changes detected fecal microbiota between but significantly increased specific IgM IgG against Severe Acute Respiratory Syndrome (SARS-CoV2) It is thus hypothesized this primarily acts interacting host's immune system rather than changing colonic composition. Future studies should replicate these findings elucidate its mechanism action (Registration: NCT04517422).Abbreviations: AE: Adverse Event; BMI: Body Mass Index; CONSORT: CONsolidated Standards Reporting Trials; CFU: Colony-Forming Units; eDRF: Electronic Daily Report Form; GLA: Gut-Lung Axis; GSRS: Gastrointestinal Symptoms Rating Scale; hsCRP: High-sensitivity C-Reactive Protein; HR: Hazard Ratio; ICU: Unit; OR: Odds PCoA: Principal Coordinate Analysis; RR: Relative Risk; RT-qPCR: Real-Time Quantitative Polymerase Chain Reaction; SARS-CoV2: acute respiratory syndrome coronavirus 2; SpO2: Peripheral Oxygen Saturation; WHO: World Health Organization.

Language: Английский

Citations

167
Manganese-catalyzed hydrogenation, dehydrogenation, and hydroelementation reactions DOI
Kuhali Das, Satyadeep Waiba, Akash Jana

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(11), P. 4386 - 4464

Published: Jan. 1, 2022

The emerging field of organometallic catalysis has shifted towards research on Earth-abundant transition metals due to their ready availability, economic advantage, and novel properties. In this case, manganese, the third most abundant transition-metal in Earth's crust, emerged as one leading competitors. Accordingly, a large number molecularly-defined Mn-complexes been synthesized employed for hydrogenation, dehydrogenation, hydroelementation reactions. regard, catalyst design is based three pillars, namely, metal-ligand bifunctionality, ligand hemilability, redox activity. Indeed, developed catalysts not only differ chelating atoms they possess but also working principles, thereby different turnover numbers product molecules. Hence, critical assessment molecularly defined manganese terms atoms, reaction conditions, mechanistic pathway, significant. Herein, we analyze complexes catalytic activity, versatility allow multiple transformations routes convert substrates target This article will be helpful get significant insight into design, aiding design.

Language: Английский

Citations

163
Mechanical overloading promotes chondrocyte senescence and osteoarthritis development through downregulating FBXW7 DOI
Haiyan Zhang, Yan Shao,

Zihao Yao

et al.

Annals of the Rheumatic Diseases, Journal Year: 2022, Volume and Issue: 81(5), P. 676 - 686

Published: Jan. 20, 2022

To investigate the role of mechanical stress in cartilage ageing and identify mechanistic association during osteoarthritis (OA) progression.F-box WD repeat domain containing 7 (FBXW7) ubiquitin ligase expression chondrocyte senescence were examined vitro, experimental OA mice human cartilage. Mice with Fbxw7 knockout chondrocytes generated adenovirus-expressing (AAV-Fbxw7) was injected intra-articularly mice. Destabilised medial meniscus surgery performed to induce OA. Cartilage damage measured using Osteoarthritis Research Society International score changes determined. mRNA sequencing articular from control mice.Mechanical overloading accelerated cultured FBXW7 downregulated by primary cartilage, decreased patients OA, aged deletion induced catabolism mice, as manifested an upregulation p16INK4A, p21 Colx downregulation Col2a1 ACAN, which resulted exacerbation By contrast, intra-articular injection adenovirus expressing alleviated Mechanistically, transcription FBXW7-mediated MKK7 degradation, consequently stimulated JNK signalling. In particular, inhibition activity DTP3, a inhibitor, ameliorated degeneration CONCLUSIONS: is key factor between pathology

Language: Английский

Citations

117
An overview of the enhanced biomass gasification for hydrogen production DOI Open Access

Nowilin James Rubinsin,

Nabila A. Karim, Sharifah Najiha Timmiati

et al.

International Journal of Hydrogen Energy, Journal Year: 2023, Volume and Issue: 49, P. 1139 - 1164

Published: Sept. 23, 2023

Language: Английский

Citations

77
Biomass Photoreforming for Hydrogen and Value‐Added Chemicals Co‐Production on Hierarchically Porous Photocatalysts DOI Creative Commons
Heng Zhao, Jing Liu, Na Zhong

et al.

Advanced Energy Materials, Journal Year: 2023, Volume and Issue: 13(21)

Published: April 7, 2023

Abstract Biomass, a naturally abundant, sustainable and clean resource has great potential as an alternative to replace the limited fossil feedstock for value‐added chemicals fuels. Biomass with abundant reductive functional groups could theoretically act electron donor consume photogenerated holes and/or active free radicals. photoreforming over semiconductor photocatalysts using solar light energy input attracts much attention in this context. However, biomass still suffers low conversion efficiency product selectivity due its structural complexity, poor solubility unclear reaction mechanism. Owing advanced features of mass diffusion derivatives adjustable surface properties, hierarchically porous desired sites at each length scale porosity have shown their superiority boosting photoreforming. Herein, critical review is presented on selective simultaneous H 2 co‐production photocatalysts. The fundamentals current bottleneck valorization by photocatalytic process are analyzed. rational photocatalyst design structure improve transfer highlighted. Finally challenges opportunities perspective ssection.

Language: Английский

Citations

65
L‐Arginine‐Loaded Gold Nanocages Ameliorate Myocardial Ischemia/Reperfusion Injury by Promoting Nitric Oxide Production and Maintaining Mitochondrial Function DOI Creative Commons
Zekun Wang, Nana Yang, Ya‐Jun Hou

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(26)

Published: July 14, 2023

Abstract Cardiovascular disease is the leading cause of death worldwide. Reperfusion therapy vital to patient survival after a heart attack but can myocardial ischemia/reperfusion injury (MI/RI). Nitric oxide (NO) ameliorate MI/RI and key molecule for drug development. However, reactive oxygen species (ROS) easily oxidize NO peroxynitrite, which causes secondary cardiomyocyte damage. Herein, L‐arginine‐loaded selenium‐coated gold nanocages (AAS) are designed, synthesized, modified with PCM (WLSEAGPVVTVRALRGTGSW) obtain AASP, targets cardiomyocytes, exhibits increased cellular uptake, improves photoacoustic imaging in vitro vivo. AASP significantly inhibits glucose deprivation/reoxygenation (OGD/R)‐induced H9C2 cell cytotoxicity apoptosis. Mechanistic investigation revealed that mitochondrial membrane potential (MMP), restores ATP synthase activity, blocks ROS generation, prevents oxidation, release by regulating closing permeability transition pore (mPTP). administration vivo function, apoptosis fibrosis, ultimately attenuates rats maintaining function signaling. shows good safety biocompatibility This findings confirm rational design provide effective treatment MI/RI.

Language: Английский

Citations

58
Recent advances in biomimetic aggregation‐induced emission photosensitizers for photodynamic therapy and immunotherapy DOI Creative Commons
Shuai Guo, Hui Tang, Yahui Zhang

et al.

BMEMat, Journal Year: 2024, Volume and Issue: 2(3)

Published: April 24, 2024

Abstract The development of novel photosensitizers (PSs) with aggregation‐induced emission (AIE) properties has emerged as a crucial advancement in the field photodynamic therapy (PDT). However, versatile applications AIE PSs are limited by low encapsulation efficiency and inadequate target tissue permeability. Biomimetic technology stands out promising strategy to overcome these challenges, aiming enhance tumor penetration efficacy, their association antitumor immune responses. In this review, recent advancements biomimetic for PDT immunotherapy summarized. We start introducing strategies involving based on cell membranes extracellular vesicles combined application immunotherapy. then discuss preparation nanoparticles. Finally, we briefly outline challenges prospects associated PSs.

Language: Английский

Citations

22
Advancements in multifunctional manganese complexes for catalytic hydrogen transfer reactions DOI
Kuhali Das, Milan Kr. Barman, Biplab Maji

et al.

Chemical Communications, Journal Year: 2021, Volume and Issue: 57(69), P. 8534 - 8549

Published: Jan. 1, 2021

Manganese catalyzed hydrogen transfer reactions enabled net reductions and cascade CC- CN-bond formation reactions. The success is aided by multifunctional ligand design namely metal–ligand bifunctionality, hemilability, redox non-innocence.

Language: Английский

Citations

61
Iron salt catalytic pyrolysis of biomass: Influence of iron salt type DOI
Sunwen Xia,

Haiping Yang,

Shuaishuai Lei

et al.

Energy, Journal Year: 2022, Volume and Issue: 262, P. 125415 - 125415

Published: Sept. 16, 2022

Language: Английский

Citations

57
Towards ligand simplification in manganese-catalyzed hydrogenation and hydrosilylation processes DOI Creative Commons

Ekaterina S. Gulyaeva,

Elena S. Osipova, Ruqaya Buhaibeh

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 458, P. 214421 - 214421

Published: Jan. 31, 2022

Language: Английский

Citations

56