Horticulture Research, Journal Year: 2022, Volume and Issue: 9
Published: Jan. 1, 2022
Language: Английский
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Feb. 20, 2024
Abstract Cepharanthine is a secondary metabolite isolated from Stephania . It has been reported that it anti-conronaviruses activities including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we assemble three genomes ( S. japonica , yunnanensis and cepharantha ), propose the cepharanthine biosynthetic pathway, assess antiviral potential of compounds involved in pathway. Among genomes, near telomere-to-telomere assembly with one remaining gap, have chromosome-level assemblies. Following by gene mining metabolomics analysis, identify seven analogs broad-spectrum anti-coronavirus activities, SARS-CoV-2, Guangxi pangolin-CoV (GX_P2V), swine diarrhoea coronavirus (SADS-CoV), porcine epidemic diarrhea virus (PEDV). We also show two other genera, Nelumbo Thalictrum can produce analogs, thus for compound discovery. Results generated this study could accelerate drug
Language: Английский
Citations
35
Natural Product Reports, Journal Year: 2022, Volume and Issue: 39(7), P. 1465 - 1482
Published: Jan. 1, 2022
Covering: up to 2022Plants produce a wide range of structurally and biosynthetically diverse natural products interact with their environment. These specialised metabolites typically evolve in limited taxonomic groups presumably response specific selective pressures. With the increasing availability sequencing data, it has become apparent that many cases genes encoding biosynthetic enzymes for metabolic pathways are not randomly distributed on genome. Instead they physically linked structures such as arrays, pairs clusters. The exact function these clusters is debated. In this review we take broad view gene arrangement plant metabolism, examining types variation. We discuss evolution wider context populations epigenetics. Finally, synthesise our observations propose new hypothesis cluster formation plants.
Language: Английский
Citations
56
Pharmacological Research, Journal Year: 2022, Volume and Issue: 177, P. 106076 - 106076
Published: Jan. 21, 2022
Drug discovery from natural sources is going through a renaissance, having spent many decades in the shadow of synthetic molecule drug discovery, despite fact that product-derived compounds occupy much greater chemical space than those created chemistry methods. With this new era comes possibilities, not least novel targets have emerged recent times and development state-of-the-art technologies can be applied to sources. Although progress has been made with some immunomodulating drugs, there remains pressing need for agents used treat wide variety conditions arise disruption, or over-activation, immune system; products may therefore key filling gap. Recognising that, at present, no authoritative article details current immunomodulatory activity products, in-depth review arisen joint effort between International Union Basic Clinical Pharmacology (IUPHAR) Natural Products Immunopharmacology Sections, contributions number world-leading researchers field product provide "position statement" on what offer search argents. To end, we historical look previous discoveries naturally occurring immunomodulators, present picture status insight into future opportunities challenges drugs immune-related diseases.
Language: Английский
Citations
55
Horticulture Research, Journal Year: 2022, Volume and Issue: 9
Published: Jan. 1, 2022
Globally, medicinal plant natural products (PNPs) are a major source of substances used in traditional and modern medicine. As we human race face the tremendous public health challenge posed by emerging infectious diseases, antibiotic resistance surging drug prices etc., harnessing healing power plants gifted from mother nature is more urgent than ever helping us survive future sustainable way. PNP research efforts pre-genomic era focus on discovering bioactive molecules with pharmaceutical activities, identifying individual genes responsible for biosynthesis. Critically, systemic biological, multi- inter-disciplinary approaches integrating interrogating all accessible data genomics, metabolomics, structural biology, chemical informatics necessary to accelerate full characterization biosynthetic regulatory circuitry producing PNPs plants. In this review, attempt provide brief update current focusing how different state-of-the-art biotechnologies facilitate their discovery, molecular basis biosynthesis, as well synthetic biology. Finally, humbly foresight trend understanding biology coming decades.
Language: Английский
Citations
43
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(24), P. 15932 - 15932
Published: Dec. 14, 2022
Medicinal plants produce important substrates for their adaptation and defenses against environmental factors and, at the same time, are used traditional medicine industrial additives. Plants have relatively little in way of secondary metabolites via biosynthesis. Recently, whole-genome sequencing medicinal identification metabolite production were revolutionized by rapid development cheap cost technology. Advances functional genomics, such as transcriptomics, proteomics, metabolomics, pave discoveries related key genes. The multi-omics approaches can offer tremendous insight into variety, distribution, biosynthetic gene clusters (BGCs). Although many reviews reported on plant genome, chemistry, pharmacology, there is no review giving a comprehensive report about genome to study biosynthesis pathway metabolites. Here, we introduce application tools identifying genes Moreover, explore comparative genomics polyploidy family analysis plants. This promotes which contributes screening plant-based drugs prompts research efficiency medicine.
Language: Английский
Citations
39
Journal of Integrative Plant Biology, Journal Year: 2023, Volume and Issue: 65(6), P. 1423 - 1441
Published: Jan. 21, 2023
Common buckwheat (Fagopyrum esculentum) and Tartary tataricum), the two most widely cultivated species, differ greatly in flavonoid content reproductive mode. Here, we report first high-quality chromosome-level genome assembly of common with 1.2 Gb. Comparative genomic analysis revealed that underwent a burst long terminal repeat retrotransposons insertion accompanied by numerous large chromosome rearrangements after divergence from buckwheat. Moreover, multiple gene families involved stress tolerance biosynthesis such as multidrug toxic compound extrusion (MATE) chalcone synthase (CHS) significant expansion buckwheat, especially Integrated multi-omics identified high expression catechin biosynthesis-related genes flower seed rutin being important for differences type between these species. We also candidate key rutin-degrading enzyme (Ft8.2377) was highly expressed seed. In addition, haplotype-resolved locus containing many reportedly associated development pollen, which potentially related to self-incompatibility Our study provides resources facilitating future functional genomics-related research
Language: Английский
Citations
27
Advanced Science, Journal Year: 2024, Volume and Issue: 11(19)
Published: March 13, 2024
Abstract Menispermaceae species, as early‐diverging eudicots, can synthesize valuable benzylisoquinoline alkaloids (BIAs) like bisbenzylisoquinoline (bisBIAs) and sinomenines with a wide range of structural diversity. However, the evolutionary mechanisms responsible for their chemo‐diversity are not well understood. Here, chromosome‐level genome assembly Menispermum dauricum is presented demonstrated occurrence two whole duplication (WGD) events that shared by Ranunculales specific to , providing model understanding chromosomal evolution in eudicots. The biosynthetic pathway diverse BIAs M. reconstructed analyzing transcriptome metabolome. Additionally, five catalytic enzymes – one norcoclaurine synthase (NCS) four cytochrome P450 monooxygenases (CYP450s) from formation skeleton, hydroxylated modification, C‐O/C‐C phenol coupling BIAs. Notably, novel leaf‐specific MdCYP80G10 enzyme catalyzes C2′‐C4a ( S )‐reticuline into sinoacutine, enantiomer morphinan compounds, predictable stereospecificity discovered. Moreover, it found ‐specific CYP80 gene expansion, tissue‐specific expression, has driven BIA diversity compared other species. This study sheds light on WGD occurrences eudicots biosynthesis.
Language: Английский
Citations
12
Cell, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
12
Molecules, Journal Year: 2024, Volume and Issue: 29(7), P. 1566 - 1566
Published: March 31, 2024
In this paper, berberine hydrochloride-loaded liposomes-in-gel were designed and developed to investigate their antioxidant properties therapeutic effects on the eczema model of mouse. Berberine hydrochloride-liposomes (BBH-L) as nanoparticles prepared by thin-film hydration method then dispersed BBH-L evenly in gel matrix prepare hydrochloride liposomes-gel (BBH-L-Gel) natural swelling method. Their capacity was investigated free radical scavenging ability 2,2-diphenyl-1-picrylhydrazyl (DPPH) H2O2 inhibition lipid peroxides malondialdehyde (MDA). An established, efficacy treatment preliminarily evaluated using ear swelling, spleen index, pathological sections indicators. The results indicate that entrapment efficiency 78.56% ± 0.7%, with a particle size 155.4 9.3 nm. For BBH-L-Gel, viscosity pH 18.16 6.34 m Pas 7.32 0.08, respectively. cumulative release unit area vitro transdermal study 85.01 4.53 μg/cm2. BBH-L-Gel had good DPPH H2O2, it could effectively inhibit production hepatic MDA concentration range 0.4–2.0 mg/mL. topical application alleviate symptoms reduce oxidative stress injury mice. This demonstrates has skin permeability, excellent sustained release, capabilities. They can itching, inflammation, allergic caused eczema, providing new strategy for clinical applications treatment.
Language: Английский
Citations
9
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 4, 2025
Language: Английский
Citations
1