Advances in understanding the regulation of pluripotency fate transition in embryonic stem cells DOI Creative Commons

Yunxi Jia,

Yang Yu, Li Guan

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 16, 2024

Embryonic stem cells (ESCs) sourced from the inner cell mass of blastocysts, are akin to this tissue in function but lack capacity form all extraembryonic structures. mESCs transient populations that express high levels transcripts characteristic 2-cell (2C) embryos and identified as “2-cell-like cells” (2CLCs). Previous studies have shown 2CLCs can contribute both embryonic tissues upon reintroduction into early embryos. Approximately 1% dynamically transition pluripotent 2CLCs. Nevertheless, scarcity mammalian presents a significant challenge molecular characterization totipotent cells. To date, explored various methods for reprogramming While there is good understanding regulatory network maintaining ES pluripotency, process by which ESCs reprogram associated mechanisms regulation remain poorly understood. This review synthesizes recent insights pathways ESC 2CLC, exploring modulated transcriptional regulators, small molecules, epigenetic changes. The objective construct theoretical framework field researchers.

Language: Английский

Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development DOI
Ling Zhang, Jing Zhao, Sin Man Lam

et al.

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(2), P. 278 - 293

Published: Feb. 1, 2024

Language: Английский

Citations

29
Nucleolar-based Dux repression is essential for embryonic two-cell stage exit DOI Open Access

Sheila Q. Xie,

Bryony Leeke,

Chad Whilding

et al.

Genes & Development, Journal Year: 2022, Volume and Issue: 36(5-6), P. 331 - 347

Published: March 1, 2022

Upon fertilization, the mammalian embryo must switch from dependence on maternal transcripts to transcribing its own genome, and in mice this involves transient up-regulation of MERVL transposons MERVL-driven genes at two-cell stage. The mechanisms requirement for (2C) gene are poorly understood. Moreover, transcriptional program be rapidly shut off allow exit developmental progression. Here, we report that robust ribosomal RNA (rRNA) synthesis nucleolar maturation essential 2C state. 2C-like cells embryos show similar immature nucleoli with altered structure reduced rRNA output. We reveal disruption via blocking polymerase I activity or preventing phase separation enhances conversion a state embryonic stem (ESCs) by detachment activator Dux surface. In embryos, prevents proper silencing leads two- four-cell arrest. Our findings an intriguing link between synthesis, maturation, repression during early development.

Language: Английский

Citations

42
Ribosome biogenesis and function in development and disease DOI Open Access
Chunyang Ni, Michael Buszczak

Development, Journal Year: 2023, Volume and Issue: 150(5)

Published: March 1, 2023

ABSTRACT Although differential transcription drives the development of multicellular organisms, ultimate readout a protein-coding gene is ribosome-dependent mRNA translation. Ribosomes were once thought as uniform molecular machines, but emerging evidence indicates that complexity and diversity ribosome biogenesis function should be given fresh look in context development. This Review begins with discussion different developmental disorders have been linked perturbations production function. We then highlight recent studies reveal how cells tissues exhibit variable levels protein synthesis, changes synthesis capacity can influence specific cell fate decisions. finish by touching upon heterogeneity stress responses These discussions importance considering both functional specialization disease.

Language: Английский

Citations

31
LINE-1, the NORth star of nucleolar organization DOI Open Access
Misaki Matsuo, Gaël Cristofari

Genes & Development, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Long interspersed element-1 (LINE-1) retrotransposons are abundant transposable elements in mammals and significantly influence chromosome structure, chromatin organization, 3D genome architecture. In this issue of Genes & Development , Ataei et al. (doi:10.1101/gad.351979.124) identify a homininae-specific LINE-1 element within nucleolar ogranizer regions (NORs) that is specifically transcribed naïve human embryonic stem cells. Deletion or silencing disrupts organization function alters cellular identity. These findings provide novel insights into the role suggest individual may have evolved specialized roles.

Language: Английский

Citations

1
LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs DOI

Juan Zhang,

Lamisa Ataei,

Kirti Mittal

et al.

Developmental Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

8
Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment DOI
Dingfeng Li, Ran Cao,

Qiaodan Li

et al.

Nature Structural & Molecular Biology, Journal Year: 2023, Volume and Issue: 30(7), P. 914 - 925

Published: May 18, 2023

Language: Английский

Citations

15
Membraneless organelles in health and disease: exploring the molecular basis, physiological roles and pathological implications DOI Creative Commons
Yangxin Li, Brian Liu,

Xi‐Yong Yu

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Nov. 18, 2024

Abstract Once considered unconventional cellular structures, membraneless organelles (MLOs), substructures involved in biological processes or pathways under physiological conditions, have emerged as central players dynamics and function. MLOs can be formed through liquid-liquid phase separation (LLPS), resulting the creation of condensates. From neurodegenerative disorders, cardiovascular diseases, aging, metabolism to cancer, influence on human health disease extends widely. This review discusses underlying mechanisms LLPS, biophysical properties that drive MLO formation, their implications for We highlight recent advances understanding how physicochemical environment, molecular interactions, post-translational modifications regulate LLPS dynamics. offers an overview discovery current biomolecular condensate conditions diseases. article aims deliver latest insights by analyzing research, highlighting critical role organization. The discussion also covers membrane-associated condensates cell signaling, including those involving T-cell receptors, stress granules linked lysosomes, within Golgi apparatus. Additionally, potential targeting clinical settings is explored, promising avenues future research therapeutic interventions.

Language: Английский

Citations

6
Molecular Mechanisms of Plant Regeneration from Differentiated Cells: Approaches from Historical Tissue Culture Systems DOI Creative Commons
Hatsune Morinaka, Duncan Coleman, Keiko Sugimoto

et al.

Plant and Cell Physiology, Journal Year: 2022, Volume and Issue: 64(3), P. 297 - 304

Published: Dec. 22, 2022

Abstract Plants can exert remarkable capacity for cell reprogramming even from differentiated cells. This ability allows plants to regenerate tissues/organs and individuals in nature vitro. In recent decades, Arabidopsis research has uncovered molecular mechanisms of plant regeneration; however, our understanding how cells retain both status developmental plasticity is still obscure. this review, we first provide a brief outlook the representative modes regeneration key factors revealed by research. We then re-examine historical tissue culture systems that enable us investigate details discuss different approaches, specifically highlighting progress shoot epidermal Torenia fournieri.

Language: Английский

Citations

17
Retrotransposon renaissance in early embryos DOI Creative Commons
Youjia Guo, Ten D. Li, A Modzelewski

et al.

Trends in Genetics, Journal Year: 2023, Volume and Issue: 40(1), P. 39 - 51

Published: Nov. 8, 2023

Language: Английский

Citations

11
Ribosomal proteins regulate 2-cell-stage transcriptome in mouse embryonic stem cells DOI Creative Commons
Yao Yi, Yingying Zeng, Tsz Wing Sam

et al.

Stem Cell Reports, Journal Year: 2023, Volume and Issue: 18(2), P. 463 - 474

Published: Jan. 12, 2023

A rare sub-population of mouse embryonic stem cells (mESCs), the 2-cell-like cell, is defined by expression MERVL and 2-cell-stage-specific transcript (2C transcript). Here, we report that ribosomal proteins (RPs) RPL14, RPL18, RPL23 maintain identity mESCs regulate 2C transcripts. Disregulation RPs induces DUX-dependent transcripts alters chromatin landscape. Mechanically, knockdown (KD) triggers binding RPL11 to MDM2, an interaction known prevent P53 protein degradation. Increased upon RP KD further activates its downstream pathways, including DUX. Our study delineates critical roles in activation, ascribing a novel function these essential proteins.

Language: Английский

Citations

10