X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT DOI Creative Commons
Arne Maes, Onno Borgel,

Clara Braconnier

et al.

International Journal of Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 15

Published: April 9, 2024

The kidney's microstructure, which comprises a highly convoluted tubular and vascular network, can only be partially revealed using classical 2D histology. Considering that the microstructure is closely related to its function often affected by pathologies, there need for powerful high-resolution 3D imaging techniques visualize microstructure. Here, we present how cryogenic contrast-enhanced microCT (cryo-CECT) allowed visualization of glomeruli, tubuli, vasculature. By comparing different contrast-enhancing staining agents freezing protocols, found preferred sample preparation protocol was combination with 1:2 hafnium(IV)-substituted Wells-Dawson polyoxometalate submersion in isopentane at -78°C. This optimized showed sensitive, allowing detect small pathology-induced microstructural changes mouse model mild trauma-related acute kidney injury after thorax trauma hemorrhagic shock. In summary, demonstrated cryo-CECT an effective histopathological tool allows enhance our understanding tissue their function.

Language: Английский

Analysis of 3D pathology samples using weakly supervised AI DOI Creative Commons
Andrew H. Song, Mane Williams, Drew F. K. Williamson

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(10), P. 2502 - 2520.e17

Published: May 1, 2024

Human tissue, which is inherently three-dimensional (3D), traditionally examined through standard-of-care histopathology as limited two-dimensional (2D) cross-sections that can insufficiently represent the tissue due to sampling bias. To holistically characterize histomorphology, 3D imaging modalities have been developed, but clinical translation hampered by complex manual evaluation and lack of computational platforms distill insights from large, high-resolution datasets. We present TriPath, a deep-learning platform for processing volumes efficiently predicting outcomes based on morphological features. Recurrence risk-stratification models were trained prostate cancer specimens imaged with open-top light-sheet microscopy or microcomputed tomography. By comprehensively capturing morphologies, volume-based prognostication achieves superior performance traditional 2D slice-based approaches, including clinical/histopathological baselines six certified genitourinary pathologists. Incorporating greater volume improves prognostic mitigates risk prediction variability bias, further emphasizing value larger extents heterogeneous morphology.

Language: Английский

Citations

24
A Lung CT Foundation Model Facilitating Disease Diagnosis and Medical Imaging DOI Creative Commons
Zebin Gao, Guoxun Zhang, Hengrui Liang

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract The concomitant development and evolution of lung computed tomography (CT) artificial intelligence (AI) has allowed non-invasive imaging to be a key part the clinical care patients with major diseases, such as cancer. However, paucity labeled CT data limited training highly efficacious AI models thereby retarded broad-scale adoption deployment AI-based in real-world setting. In this paper, We introduce LCTfound, foundational model that encodes images along correlated information, into neural network. LCTfound used self-supervised learning pre-trained by diffusion using large dataset containing 105,184 scans (totaling more than 28 million images) from multiple centers. was evaluated on 8 categories tasks, ranging scanning-level diagnosis pixel-level image restoration, including segmentation mediastinal neoplasm, pulmonary alveolar proteinosis, prognosis non-small cell cancer, prediction pathological response neoadjuvant chemoimmunotherapy, whole 3D modeling for surgical navigation, virtual angiography(CTA), reconstruction sparse views, enhancement low-dose images. Equipped robust few-shot capability, outperformed previously state-of-the-art all above tasks. is advancements representation CT, laying groundwork operates high efficacy across spectrum low-level high-level tasks serving dual purpose aiding diseases improving quality imaging.

Language: Английский

Citations

1
Contrast-Enhancing Staining Agents for ex vivo Contrast-Enhanced Computed Tomography: a review DOI Creative Commons
Tim Balcaen, Sarah Vangrunderbeeck, Wim M. De Borggraeve

et al.

Tomography of Materials and Structures, Journal Year: 2025, Volume and Issue: 7, P. 100052 - 100052

Published: Feb. 1, 2025

Language: Английский

Citations

1
Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues DOI Creative Commons
Arne Maes, Camille Pestiaux, Alice Marino

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 20, 2022

Abstract Biological tissues comprise a spatially complex structure, composition and organization at the microscale, named microstructure. Given close structure-function relationships in tissues, structural characterization is essential to fully understand functioning of healthy pathological as well impact possible treatments. Here, we present nondestructive imaging approach perform quantitative 3D histo(patho)logy biological termed Cryogenic Contrast-Enhanced MicroCT (cryo-CECT). By combining sample staining, using an X-ray contrast-enhancing staining agent, with freezing optimal rate, cryo-CECT enables visualization analysis individual tissue constituents, such muscle collagen fibers. We applied on murine hearts subjected pressure overload following transverse aortic constriction surgery. Cryo-CECT allowed analyze, unprecedented manner, orientation diameter fibers entire heart, localization fibrotic regions within myocardial layers. foresee further applications optimization tissue/food preservation donor banking, showing that also has clinical industrial potential.

Language: Английский

Citations

35
Composite of bacterial cellulose and gelatin: A versatile biocompatible scaffold for tissue engineering DOI
Igor Rzhepakovsky, Sergey Piskov, Svetlana Avanesyan

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 256, P. 128369 - 128369

Published: Nov. 23, 2023

Language: Английский

Citations

17
Multimodal experimental studies of the passive mechanical behavior of human aortas: Current approaches and future directions DOI Creative Commons
Anna Pukaluk, Gerhard Sommer, Gerhard A. Holzapfel

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 178, P. 1 - 12

Published: Feb. 22, 2024

Cardiovascular diseases are the leading cause of death worldwide and include, among others, critical conditions aortic wall. Importantly, such require effective diagnosis treatment, which not yet accurate enough. However, they could be significantly strengthened with predictive material models In particular, support surgical decisions, preoperative planning, estimation postoperative tissue remodeling. developing a model requires experimental data showing both structural parameters mechanical behavior. Such can obtained using multimodal experiments. This review therefore discusses current approaches to strength wall is determined primarily by its passive components, i.e., mainly collagen, elastin, proteoglycans. Therefore, this focuses on experiments that relate behavior human structure organization components. classified according expected results. Multiple examples provided for each class summarized highlighted advantages disadvantages method. Finally, future directions envisioned evaluated. Multimodal innovative have gained interest very quickly, but also recently. presents first clear summary groundbreaking research in field The benefits limitations various types thoroughly discussed, comprehensive overview possible results provided. Although performed tissues, methods used described limited tissues extended other soft materials.

Language: Английский

Citations

7
Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain DOI Creative Commons
Shelei Pan, Peter Yang,

Dakota DeFreitas

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 27, 2023

Abstract Cerebrospinal fluid (CSF) is essential for the development and function of central nervous system (CNS). However, brain its interstitium have largely been thought as a single entity through which CSF circulates, it not known whether specific cell populations within CNS preferentially interact with CSF. Here, we develop technique tracking, gold nanoparticle-enhanced X-ray microtomography, to achieve micrometer-scale resolution visualization circulation patterns during development. Using this method subsequent histological analysis in rodents, identify previously uncharacterized pathways from subarachnoid space (particularly basal cisterns) that mediate CSF-parenchymal interactions involving 24 functional-anatomic groupings spinal cord. distribution these areas restricted early altered posthemorrhagic hydrocephalus. Our study also presents particle size-dependent including interaction between neurons small tracers, but large tracers. These findings implications understanding biological basis normal pathogenesis broad range disease states,

Language: Английский

Citations

14
Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study DOI Creative Commons
Alberto Sensini, Olga Stamati, Gregorio Marchiori

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e26796 - e26796

Published: Feb. 24, 2024

Regeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made poly-L(lactic) acid/collagen blend were developed reproducing all the multiscale levels aggregation these tissues. Scanning electron microscopy, microCT tensile mechanical tests carried out, including digital volume correlation analysis to measure full-field strain distribution structures. The principal strains (εp1 εp3) described pattern caused by nanofibers rearrangement, while deviatoric revealed related internal sliding bundles. results confirmed biomimicry such scaffolds, paving way further tissue engineering clinical applications.

Language: Английский

Citations

6
Internal and interfacial microstructure characterization of ice droplets on surfaces by X-ray computed tomography DOI
Laurens Snels, Navid Mostofi Sarkari, Jeroen Soete

et al.

Journal of Colloid and Interface Science, Journal Year: 2023, Volume and Issue: 637, P. 500 - 512

Published: Jan. 21, 2023

Language: Английский

Citations

13
Human lung virtual histology by multi-scale x-ray phase-contrast computed tomography DOI Creative Commons
Jakob Reichmann, Stijn E. Verleden, Mark Kühnel

et al.

Physics in Medicine and Biology, Journal Year: 2023, Volume and Issue: 68(11), P. 115014 - 115014

Published: May 11, 2023

Abstract Objectives. As the central organ of respiratory system, human lung is responsible for supplying oxygen to blood, which reaches erythrocytes by diffusion through alveolar walls and then distributed throughout body. By exploiting difference in electron density detected a phase shift soft tissue, high-resolution x-ray phase-contrast computed tomography (XPCT) can resolve biological structures sub- μ m range, shedding new light on three-dimensional structure lungs, physiological functions pathological mechanisms. Approach. This work presents both synchrotron laboratory XPCT results postmortem tissue from autopsies biopsies embedded with various preparation protocols such as precision-cut slices, cryogenically fixed well paraffin alcohol tissue. The selection abnormalities includes channel Lambert, bronchus-associated lymphoid capillary dysplasia misalignment pulmonary veins. Subsequently, quantification visualization approaches are presented. Main results. overall high image quality even in-house scans case FFPE be exploited wide range pathologies translated dedicated optimized instrumentation could operated clinical setting. using radiation, contrast further increased sized features down sub-cellular level. demonstrate that including sample mounting liquids used. Significance. With XPCT, poorly understood 3D identified larger volume overview subsequently studied more detail at higher resolution. full structure, respective airways or vascular networks, different pathophysiologic mechanisms elucidated least underpinned structural data. Moreover, data used validate provide ground truth standardizing method.

Language: Английский

Citations

11