SMAP3-ID for Identification of Endogenous Protein–Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins DOI Creative Commons
Julia T. Warren, Jian Wang,

Francis Dhoro

et al.

JACS Au, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Proteins regulate biological functions through the formation of distinct protein complexes. Identification and characterization these protein–protein interactions are critical to deciphering their mechanism action. Different antibody-based or cross-linking-based methods have been developed identify interactions. However, require genetic engineering other means disrupt native environments. To circumvent this limitation, we introduce here SMAP3-ID (small-molecule-assisted identification proximity) method in cellular environment. This combines a selective ligand for binding interest photo-cross-linking, live-cell-compatible bioorthogonal click reaction with trifunctional chemical probe, final photo-cross-linking covalently capture interacting proteins. Using nuclear lamins as an example, identified numerous lamin interactors cells. Significantly, number mitochondrial enzymes novel A (LA) interactors. The between LA were further validated, which provides mechanistic insights underlying metabolic alterations caused by mutations LA. Furthermore, our previously described small-molecule LA, LBL1, also induced changes activity bioenergetic organization. We conclude that is potentially powerful generalizable

Language: Английский

Targeting ROS in cancer: rationale and strategies DOI
Christophe Glorieux, Shihua Liu, Dunyaporn Trachootham

et al.

Nature Reviews Drug Discovery, Journal Year: 2024, Volume and Issue: 23(8), P. 583 - 606

Published: July 9, 2024

Language: Английский

Citations

137
Dux activates metabolism-lactylation-MET network during early iPSC reprogramming with Brg1 as the histone lactylation reader DOI Creative Commons
Xinglin Hu, Xingwei Huang, Yue Yang

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(10), P. 5529 - 5548

Published: March 21, 2024

Abstract The process of induced pluripotent stem cells (iPSCs) reprogramming involves several crucial events, including the mesenchymal-epithelial transition (MET), activation genes, metabolic reprogramming, and epigenetic rewiring. Although these events intricately interact influence each other, specific element that regulates network remains unclear. Dux, a factor known to promote totipotency during from embryonic (ESC) 2C-like ESC (2CLC), has not been extensively studied in context iPSC reprogramming. In this study, we demonstrate modification H3K18la by Dux overexpression controls metabolism-H3K18la-MET network, enhancing efficiency through switch recruitment p300 via its C-terminal domain. Furthermore, our proteomic analysis immunoprecipitation experiment uncovers Brg1 with both being enriched on promoters genes associated pluripotency epithelial junction. summary, study demonstrated significant role Dux-induced early process, highlighting function as potent trigger. Additionally, research revealed, for first time, binding H3K18la, indicating reader histone lactylation.

Language: Английский

Citations

24
A novel protein CYTB-187AA encoded by the mitochondrial gene CYTB modulates mammalian early development DOI

Zhijuan Hu,

Liang Yang,

Maolei Zhang

et al.

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(7), P. 1586 - 1597.e7

Published: May 3, 2024

Language: Английский

Citations

22
Oxidative stress and metabolism meet epigenetic modulation in physical exercise DOI Creative Commons
José Luis García‐Gimenez, Irene Cánovas-Cervera, Federico V. Pallardó

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 213, P. 123 - 137

Published: Jan. 8, 2024

Physical exercise is established as an important factor of health and generally recommended for its positive effects on several tissues, organs, systems. These come from metabolic adaptations that also include oxidative eustress, in which physical activity increases ROS production antioxidant mechanisms, although this depends the intensity exercise. Muscle metabolism through mechanisms such aerobic anaerobic glycolysis, tricarboxylic acid cycle, lipid can produce metabolites co-factors directly impact epigenetic machinery. In review, we clearly reinforce evidence regulates explain how these be regulated by products produced during fact, recent has demonstrated importance epigenetics gene expression changes implicated adaptation after Importantly, intermediates generated continuous, acute, moderate, or strenuous control enzymes, therefore turning off specific programs lead to physiological

Language: Английский

Citations

17
Copper homeostasis and copper-induced cell death： Novel targeting for intervention in the pathogenesis of vascular aging DOI Open Access
Zhuoying Chen, Yuanyuan Li, Xiangjie Liu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 169, P. 115839 - 115839

Published: Nov. 16, 2023

Copper-induced cell death, also known as cuproptosis, is distinct from other types of death such apoptosis, necrosis, and ferroptosis. It can trigger the accumulation lethal reactive oxygen species, leading to onset progression aging. The significant increases in copper ion levels aging populations confirm a close relationship between homeostasis vascular On hand, closely related occurrence various cardiovascular diseases throughout process. However, specific causes are not clear, different living environments stress patterns lead individualized By exploring correlations copper-induced aging, we gain novel perspective on pathogenesis enhance prognosis atherosclerosis. This article aims provide comprehensive review impacts including their effects endothelial cells, smooth muscle oxidative stress, ferroptosis, intestinal flora, factors. Furthermore, intend discuss potential strategies involving cuproptosis new insights for copper-related

Language: Английский

Citations

23
Mitochondrial homeostasis: shaping health and disease DOI Creative Commons
Xiaoyu Li, Ou Jiang, Mo Chen

et al.

Current Medicine, Journal Year: 2024, Volume and Issue: 3(1)

Published: April 15, 2024

Abstract Mitochondria serve as the primary site for metabolizing three major nutrients, underscoring their pivotal role in cellular energy metabolism and regulation of signaling pathways. Mitochondrial homeostatic imbalance is a key pathological cause development many diseases. Hence, preserving mitochondrial homeostasis vital normal growth cells organisms. Living organisms have evolved intricate regulatory mechanisms to ensure homeostasis. This review focuses on recent advancements comprehending responsible maintaining addresses current challenges this field. We also provide an overview functions mitochondria both physiological conditions. Emphasizing potential therapeutic implications, we discuss strategies homeostasis, recognizing its significance mitigating various health Graphical

Language: Английский

Citations

9
Influence mechanisms underlying the degradation of petroleum hydrocarbons in response to various nitrogen dosages supplementation through metatranscriptomics analysis DOI

Yawen Ou,

Manli Wu,

Ying Yu

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 487, P. 137074 - 137074

Published: Jan. 2, 2025

Language: Английский

Citations

1
Mechanisms of metabolism-coupled protein modifications DOI
Bingsen Zhang, Frank C. Schroeder

Nature Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

Citations

1
A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cells DOI
Joseph J. Smith, Taylor R. Valentino,

Austin H. Ablicki

et al.

Cell chemical biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Glucose metabolism and endometrium decidualization DOI Creative Commons

Yunfei Huang,

Qinling Zhu,

Yun Sun

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 17, 2025

Prior to embryo implantation, the endometrial stromal cells (ESCs) during menstrual cycle undergo a significant structural and functional transformation known as decidualization support conception. During this process, glucose consumption utilization by increase meet energy demands. Abnormal metabolism in endometrium impairs decidualization, leading pregnancy complications, including implantation failure loss. However, mechanisms modulating are still unclear. In review, we describe functions regulation of transporters (GLUTs) involved uptake, well modulation key enzymes catalyzing utilization. Moreover, present recent findings on role related metabolites ESCs.

Language: Английский

Citations

1