We need to talk—how muscle stem cells communicate

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: July 10, 2024

Skeletal muscle is one of the tissues with highest ability to regenerate, a finely controlled process which critically depending on stem cells. Muscle cell functionality depends intrinsic signaling pathways and interaction their immediate niche. Upon injury quiescent cells get activated, proliferate fuse form new myofibers, involving multiple types in regenerating skeletal muscle. Receptors receive respective signals through direct cell-cell interaction, via secreted factors or cell-matrix interactions thereby regulating responses external stimuli. Here, we discuss how interact niche focusing this controls quiescence, activation self-renewal these processes are altered age disease.

Language: Английский

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Ucp1 Ablation Improves Skeletal Muscle Glycolytic Function in Aging Mice

Advanced Science, Journal Year: 2024, Volume and Issue: 12(2)

Published: Nov. 21, 2024

Abstract Muscular atrophy is among the systematic decline in organ functions aging, while defective thermogenic fat functionality precedes these anomalies. The potential crosstalk between adipose tissue and muscle during aging poorly understood. In this study, it showed that UCP1 knockout (KO) mice characterized deteriorated brown (BAT) function yet their glucose homeostasis sustained energy expenditure increased, possibly compensated by improved inguinal (iWAT) compared to age‐matched WT mice. To understand crosstalk, RNA‐seq metabolomic analysis were performed on revealed creatine levels are increased both iWAT of KO Interestingly, molecular metabolite tracing biosynthesis uptake mice, suggesting transportation from muscle. Importantly, analog β‐GPA abolished differences inhibitor α‐CD glycolytic metabolism Overall, results suggested skeletal compensate for declined BAT via sustain metabolic homeostasis.

Language: Английский

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Dynamics of tissue repair regulatory T cells and damage in acute Trypanosoma cruzi infection

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(1), P. e1012906 - e1012906

Published: Jan. 30, 2025

Tissue-repair regulatory T cells (trTregs) comprise a specialized cell subset essential for tissue homeostasis and repair. While well-studied in sterile injury models, their role infection-induced damage antimicrobial immunity is less understood. We investigated trTreg dynamics during acute Trypanosoma cruzi infection, marked by extensive strong CD8+ immunity. Unlike trTregs significantly declined secondary lymphoid organs non-lymphoid target tissues correlating with systemic local damage, downregulation of function-associated genes skeletal muscle. This decline was linked to decreased IL-33 levels, key growth factor, promoted the Th1 cytokine IFN-γ. Early recombinant treatment increased trTregs, type 2 innate cells, parasite-specific at specific time points after leading reduced lower parasite burden, improved disease outcome. Our findings not only provide novel insights into infection but also highlight potential optimizing immune balance modulating responses promote repair while maintaining effective pathogen control injury.

Language: Английский

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3D Bioprinting‐Assisted Tissue Assembly of Endocrine Adipose Units for Enhanced Skin Regeneration

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Despite the growing recognition of adipose tissue as an endocrine organ, engineering it remains a challenge owing to difficulties in replicating its native structure and densely packed lipid droplets. Furthermore, integrating with other tissues, though critical for function, underexplored, limiting understanding roles metabolic homeostasis repair. This study introduces rapid printing method that constructs units by extruding preadipocyte‐laden bioink within 0.3 s using modular polycaprolactone framework optimized through rheological computational analyses. In standard, cell‐friendly environments, preadipocytes typically proliferate migrate, inhibiting formation dense To address this issue, hybrid limits cell migration promotes adipocyte maturation is developed. The optimal unit diameter (≤ 600 µm) calculated, adipogenic markers evaluated various spatial configurations. Tissue assembly integrates module dermis module, validating functionality tissue. vivo studies show activity significantly enhances wound closure, vascularization, re‐epithelialization. These findings highlight regenerative capabilities proposed strategy fabricating large‐scale, multicellular, 3D composite tissues.

Language: Английский

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The role of oxidative stress-mediated fibro-adipogenic progenitor senescence in skeletal muscle regeneration and repair

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 1, 2025

Stem cells play a pivotal role in tissue regeneration and repair. Skeletal muscle comprises two main stem cells: (MuSCs) fibro-adipogenic progenitors (FAPs). FAPs are essential for maintaining the regenerative milieu of modulating activation satellite cells. However, during acute skeletal injury, alterations mechanisms action remain unclear. we employed GEO database bioinformatics analysis injury. A injury model was established through cardiotoxin (CTX, 10µM, 50µL) injection into tibialis anterior (TA) C57BL/6 mice. Three days post-injury, extracted TA, isolated (CD31-CD45-PDGFRα+Sca-1+), assessed senescence phenotype SA-β-Gal staining Western blot. Additionally, co-culture system to evaluate capacity facilitate MuSCs differentiation. Finally, alleviated senescent vitro (100 µM melatonin, 5 days) vivo (20 mg/kg/day 15 administration experiments, confirming melatonin's repair processes muscle. In single-cell RNA sequencing analysis, discovered upregulation senescence-related pathways following Immunofluorescence revealed co-localization markers injured muscles. We CTX observed reduction number accompanied by manifestation phenotype. Melatonin treatment found attenuate injury-induced FAPs. Further experiments that melatonin facilitated restoration FAPs' promote myoblast Through GO KEGG led AMPK pathway FAPs, associated with antioxidant stress response. drug corroborated enhances alleviating FAP vivo. this study, first underwent redox homeostasis imbalance after Next, utilized enhance capabilities activating signaling pathway. Taken together, work provides novel theoretical foundation treating

Language: Английский

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In vivo evaluation of decellularized skeletal muscle matrices for skeletal muscle repair: A systematic review

Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Volumetric muscle loss is the significant of skeletal volume beyond innate regenerative capacity, resulting in functional impairment. The current standard care combines autografting with physical therapy but often insufficient to reach full recovery. Decellularized (DSM) provides an interesting alternative repair volumetric loss. native structure and composition extracellular matrix these acellular implants provide a blueprint for regeneration. Moreover, DSM can be combined cells facilitate regeneration defect. This systematic review complete thorough overview state‐of‐the‐art applications efficacy matrices vivo, selected according Preferred Reporting Items Systematic Reviews Meta‐Analyses guidelines. Technical information on different methods create implantation studies provided. details evaluation structural defect after are described. Results reveal large heterogeneity analysis upon implantation. makes it difficult fully assess efficiency regenerate muscle, hampering further translation this technique. Therefore, we suggest multi‐level method (i) regeneration, (ii) vascularization, (iii) innervation regenerated (iv) quantitative way.

Language: Английский

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Effects of photobiomodulation on adipocytic infiltration in sites of skin healing: in vivo experimental study

Lasers in Medical Science, Journal Year: 2025, Volume and Issue: 40(1)

Published: March 20, 2025

Language: Английский

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Isolation of adipose stromal cells from blood using a two-step microfluidic platform ASCfinder

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Mesenchymal stromal cells (MSCs) hold significant promise for their therapeutic potential and possible role as disease biomarkers. While evidence suggests the presence of circulating Adipose-derived MSC (ASC) in peripheral blood (PB), isolating them is particularly challenging due to low abundance, size variability, incomplete characterization native immunophenotype PB. Consequently, relationship between ASC frequency various physiological or pathological conditions has been underexplored. In this study, we introduce ASC-Finder, a label-free isolation method specifically designed adipose (ASCs), key population. ASC-Finder integrates two independent modules: size-dependent hydrodynamic filtration unit sorting erythrocytes directly from PB negative enrichment module based on immunological markers deplete remaining leukocytes. The device enabled removal 99.98% while achieving high recovery rates spiked ASCs (> 81%) at rare-event concentrations (< 100 ASC/mL blood). Remarkably, operates without clogging, even after multiple runs with donor samples. Crucially, our bypasses need harsh lysis, centrifugation, dilution buffers, preserving both cell integrity phenotype—key factors discovery novel cellular events. This work represents advancement direct whole offering crucial step toward investigating blood-circulating ASCs.

Language: Английский

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Adipose-derived EVs loaded resolvins biopotentiated decellularized extracellular matrix hydrogels accelerate muscle regeneration

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163325 - 163325

Published: May 1, 2025

Language: Английский

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Glycations on Decellularized Muscle Matrix Reduce Muscle Regeneration and Increase Inflammation

Tissue Engineering Part A, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Volumetric muscle loss (VML) due to traumatic injury results in the abrupt of contractile units, stem cells, and connective tissue, leading long-term dysfunction reduced regenerative potential. Muscle tissue contains a proregenerative extracellular matrix (ECM), our lab harnesses capacity decellularized (DMM) treat VML, condition with limited treatment options. However, major limitation is that often comes from aged donors. Previous work showed donor higher levels advanced glycation end-product (AGE) cross-links compared younger This study aimed determine whether increased AGE reduce DMM. To test this, we first generated AGEs DMM direct D-ribose incubation. We then removed ∼35% gastrocnemius model treated it either AGE-DMM or standard (no AGEs), comparing controls. Although force remained unchanged between DMM, led mass histological sections, fewer fibers, smaller fiber diameters. also collagen histology, but protein assays production. investigated canonical receptor for AGEs, (RAGE), found elevated AGE-treated VML alone, along noncanonical galectin-3. Both RAGE galectin-3 are associated inflammation, proteomics revealed inflammatory markers than alone. In conclusion, data suggest impair potential highlighting importance considering age when sourcing therapies. Impact Statement investigates skeletal (ECM) as way its deleterious effects on regeneration vivo. demonstrate here ECM glycations regeneration, enhance markers, production, proteomic analysis identified unique targets could be explored future research endeavors.

Language: Английский

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