Talanta, Journal Year: 2024, Volume and Issue: 274, P. 126120 - 126120
Published: April 16, 2024
Language: Английский
Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(43), P. 23727 - 23738
Published: Oct. 20, 2023
Parkinson's disease (PD) is a progressive neurodegenerative disorder causing the loss of dopaminergic neurons in substantia nigra and drastic depletion dopamine (DA) striatum; thus, DA can act as marker for PD diagnosis therapeutic evaluation. However, detecting brain not easy because its low concentration difficulty sampling. In this work, we report fabrication covalent organic framework (COF)-modified carbon fiber microelectrode (cCFE) that enables real-time detection mouse thanks to outstanding antibiofouling antichemical fouling ability, excellent analytical selectivity, sensitivity offered by COF modification. particular, inhibit polymerization on electrode (namely, chemical fouling) spatially confining molecular conformation electrochemical oxidation DA. The cCFE stably continuously work detect monitor variation concentration. Furthermore, it was combined with levodopa administration devise closed-loop feedback mode therapy, which monitors model instruct dose injection time levodopa, allowing customized medication improve efficacy meanwhile avoid adverse side effects. This demonstrates fascinating properties fabricating sensors vivo bioanalysis. We believe structural tunability diversity will offer enormous promise selective neurotransmitters brain.
Language: Английский
Citations
44
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2023, Volume and Issue: 304, P. 123377 - 123377
Published: Sept. 9, 2023
Language: Английский
Citations
24
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 174, P. 117685 - 117685
Published: April 2, 2024
Language: Английский
Citations
11
Frontiers in Behavioral Neuroscience, Journal Year: 2024, Volume and Issue: 18
Published: Aug. 9, 2024
The nucleus accumbens (NAc), a central component of the brain's reward circuitry, has been implicated in wide range behaviors and emotional states. Emerging evidence, primarily drawing from recent rodent studies, suggests that function NAc aversion processing is multifaceted. Prolonged stress or drug use induces maladaptive neuronal which results pathological conditions. This review aims to provide comprehensive up-to-date insights on role motivated behavior regulation highlights areas demand further in-depth analysis. It synthesizes latest findings how distinct populations pathways contribute opposite valences. examines neuromodulators, especially monoamines, influence NAc's control over various motivational Furthermore, it delves into complex underlying mechanisms psychiatric disorders such as addiction depression evaluates prospective interventions restore functionality.
Language: Английский
Citations
9
Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 30, 2025
Host-guest supramolecular fluorescence probes have garnered significant attention in the detection and sensing of bioactive molecules due to their functionalization potential, adjustable physical properties, high specificity. However, such that reliably, rapidly, specifically measure neurotransmitter dynamics at cellular vivo level yet be reported. Herein, we present a fluorescent chemosensor designed for norepinephrine (NE) detection, showing an exceptional response specificity through host-guest complexation. Multiple covalent/noncovalent interactions, molecular-folding, confinement effect system synergistically enhance selectivity accelerate reaction kinetics down 190 ms. Our enables real-time quantification imaging NE across various models including neuronal cytomembranes, brain tissues, zebrafish. Notably, successfully monitored levels 26 regions freely moving mice under fear-induced stress, revealing elevated concentrations these associated with emotional processing. Thus, our is robust tool measuring diverse model organisms.
Language: Английский
Citations
1
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: Feb. 21, 2025
Abstract Accurately visualizing epinephrine (EP) activity is essential for understanding its physiological functions and pathological processes in brain. However, to the best of our knowledge, reliable, rapid, specifical measurement EP dynamics at cellular vivo level hasn’t been previously reported. Herein, we report probe imaging biosensing neurons living brain freely behaving animals, based on creating a series supramolecular fluorescent chemodosimeters through host-guest interaction. The optimized chemodosimeter enables real-time quantifying with high specificity, sensitivity, signal-to-noise ratio, rapid kinetics (~240 ms) neurons, tissues zebrafish. More significantly, demonstrate monitoring 26 regions within deep male mice, unraveling an augmented concentration amygdala, thalamus, hypothalamus, hippocampus striatum under fear-induced stress. These findings highlight as powerful tool precise measurements diverse model organisms.
Language: Английский
Citations
1
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 168, P. 117327 - 117327
Published: Sept. 23, 2023
Language: Английский
Citations
23
Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(43), P. 23832 - 23841
Published: Oct. 18, 2023
Norepinephrine (NE) is a key neurotransmitter in the central and sympathetic nervous systems, whose content fluctuates dynamically rapidly various brain regions during different physiological pathophysiological processes. However, it remains great challenge to directly visualize precisely quantify transient NE dynamics living systems with high accuracy, specificity, sensitivity, and, particular, temporal resolution. Herein, we developed series of small-molecular probes that can specifically detect through sequential nucleophilic substitution–cyclization reaction, accompanied by ratiometric near-infrared fluorescence response, within an impressively short time down 60 ms, which 3 orders magnitude faster than present probes. A unique water-promoted intermolecular proton transfer mechanism disclosed, dramatically boosted recognition kinetics ∼680 times. Benefiting from these excellent features, quantitatively imaged endogenous under external stimuli at single neuron level further revealed close correlations between fluctuations Parkinson's disease pathology acute slices live mouse brains vivo.
Language: Английский
Citations
22
Nano Research, Journal Year: 2024, Volume and Issue: 17(7), P. 6443 - 6474
Published: April 12, 2024
Language: Английский
Citations
6
National Science Review, Journal Year: 2024, Volume and Issue: 12(2)
Published: Oct. 29, 2024
ABSTRACT Organic red/near-infrared (NIR) room-temperature phosphorescence (RTP) holds significant potential for autofluorescence-free bioimaging and biosensing due to its prolonged persistent luminescence exceptional penetrability. However, achieving activatable red/NIR organic RTP probes with tunable emission in aqueous solution remains a formidable challenge. Here we report on intensity lifetime amplification. These consist of supramolecular assemblies comprising macrocyclic cucurbit[8]uril amine-containing alkyl-bridged pyridiniums, exhibiting viscosity-activatable enhanced quantum yield (≤20%) lifetime. Notably, by utilizing this probe, successfully achieve two-photon imaging lysosomal viscosity millisecond-scale time-resolved cell imaging. Moreover, intravital using an probe enables the monitoring variations inflammatory mice, demonstrating significantly improved signal-to-background ratio compared fluorescence This platform facilitates versatile high-resolution vivo tracking specific biomarkers physiological events.
Language: Английский
Citations
5