Identification of novel interacting partners of the TRIM29 E3-ubiquitin ligase in immortalized cells of normal prostate basal epithelium DOI Creative Commons
Rinat Sultanov, A. Mulyukina, Victoria О. Shender

et al.

Genes and Cells, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

BACKGROUND: Based on the most recent findings, E3 ubiquitin ligase TRIM29 participates in numerous cellular processes and holds significance both formation of normal basal epithelium prostate cancer. Exploring these molecular mechanisms could be pivotal developing novel cancer therapies. AIM: Identification protein partners its truncated forms, followed by functional analysis proteins. key involving TRIM29. METHODS: Cell cultures from with overexpression chimeric TRIM29-FLAG or forms lacking B-Box domain Coiled-Coil were conducted. Protein identified through immunoprecipitation proteomic (HPLC-MS/MS) analysis. The findings validated Western blot immunocytochemistry. RESULTS: interacts 288 proteins epithelium. Deletion has minimal impact TRIM29's interactions, while deletion significantly reduces disrupts dimerization. Our research indicates that is present nucleus cytoplasm, domains does not affect localization but alters binding to compartment-specific binds cytoskeletal proteins, stress response RNA-binding Furthermore, enhances cell resistance genotoxic agents influences RNA splicing. CONCLUSION: Proteomic reveals epithelium, E3-ubiquitin a broad spectrum distinct functions various compartments. align other studies demonstrating involvement rearrangement, viral infections, DNA damage. Additionally, our demonstrates interaction potential role regulating splicing, particularly domain.

Language: Английский

Stress granule and P-body clearance: Seeking coherence in acts of disappearance DOI
J. Ross Buchan

Seminars in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 159-160, P. 10 - 26

Published: Jan. 25, 2024

Language: Английский

Citations

9
Navigating the landscape of plant proteomics DOI Open Access
Tian Sang, Zhen Zhang,

Guting Liu

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

In plants, proteins are fundamental to virtually all biological processes, such as photosynthesis, signal transduction, metabolic regulation, and stress responses. Studying protein distribution, function, modifications, interactions at the cellular tissue levels is critical for unraveling complexities of these pathways. Protein abundance localization highly dynamic vary widely across proteome, presenting a challenge global quantification analysis. Mass spectrometry-based proteomics approaches have proven be powerful tools addressing this complex issue. review, we summarize recent advancements in research their applications plant biology, with an emphasis on current state challenges studying post-translational single-cell proteomics, protein-protein interactions. Additionally, discuss future prospects highlighting potential opportunities that technologies offer advancing biology research.

Language: Английский

Citations

1
Disruption of G3BP1 granules promotes mammalian CNS and PNS axon regeneration DOI Creative Commons
Pabitra K. Sahoo, Manasi Agrawal, Nicholas J. Hanovice

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(9)

Published: Feb. 27, 2025

Depletion or inhibition of core stress granule proteins, G3BP1 in mammals and TIAR-2 Caenorhabditis elegans , increases the growth spontaneously regenerating axons. Inhibition by expression its acidic “B-domain” accelerates axon regeneration after nerve injury, bringing a potential therapeutic strategy for peripheral repair. Here, we asked whether is viable to promote injured mammalian central nervous system (CNS) where axons do not regenerate spontaneously. B-domain was found transected spinal cord provided with permissive graft (PNG) as well crushed optic nerve. Moreover, cell-permeable peptide (CPP) subregion (rodent amino acids 190 208) accelerated injury promoted regrowth reticulospinal into distal through bridging PNG. CPP from rodent human neurons cultured on substrates, this function required alternating Glu/Asp-Pro repeats that impart unique predicted tertiary structure. The disassembles axonal G3BP1, G3BP2, FMRP, but FXR1, granules selectively protein synthesis cortical neurons. These studies identify key regulator CNS demonstrate disassembly these promotes retinal elongation environments injury. This work highlights enhancing neural

Language: Английский

Citations

1
ALS-FUS mutations cause abnormal PARylation and histone H1.2 interaction, leading to pathological changes DOI Creative Commons

Hafiza Alirzayeva,

Rute Loureiro, Seda Koyuncu

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(8), P. 114626 - 114626

Published: Aug. 1, 2024

The majority of severe early-onset and juvenile cases amyotrophic lateral sclerosis (ALS) are caused by mutations in the FUS gene, resulting rapid disease progression. Mutant accumulates within stress granules (SGs), thereby affecting dynamics these ribonucleoprotein complexes. Here, we define interactome mutant

Language: Английский

Citations

5
Regulation of physiological and pathological condensates by molecular chaperones DOI Creative Commons

Nadeen Akaree,

Valentina Secco,

Flonia Levy‐Adam

et al.

FEBS Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Biomolecular condensates are dynamic membraneless compartments that regulate a myriad of cellular functions. A particular type physiological condensate called stress granules (SGs) has gained increasing interest due to its role in the response and various diseases. SGs, composed several hundred RNA‐binding proteins, form transiently protect mRNAs from translation disassemble when subsides. Interestingly, SGs contain aggregation‐prone such as TDP‐43, FUS, hnRNPA1, others, which typically found pathological inclusions seen autopsy tissues amyotrophic lateral sclerosis (ALS) frontotemporal dementia (FTD) patients. Moreover, mutations these genes lead familial ALS FTD. This led researchers propose aggregation is seeded by aberrant SGs: fail properly disassemble, lose their properties, become finally ‘mature’ into aggregates. Here, we discuss evidence supporting this model for ALS/FTD‐associated proteins. We further continue focus on molecular chaperone‐mediated regulation one hand, other. In addition review ALS/FTD‐relevant nuclear condensates, namely paraspeckles, anisosomes, nucleolar amyloid bodies, emerging chaperones. As majority chaperoning mechanisms disassembly, highlight parallel themes condensation across different chaperone families, underscoring potential early disease intervention.

Language: Английский

Citations

0
Selective Translation Under Heat Shock: Integrating HSP70 mRNA Regulation with Cellular Stress Responses in Yeast and Mammals DOI

Talar Ghadanian,

Shruti Iyer, Luca Lazzari

et al.

Molecular Biology of the Cell, Journal Year: 2025, Volume and Issue: 36(5)

Published: April 8, 2025

Under stress, cells orchestrate a complex regulatory response to maintain protein homeostasis, leveraging differential translational regulation for constitutively expressed mRNAs and the transcriptionally induced heat shock HSP70 transcripts. Constitutive typically experience partial suppression, consistent with their partitioning into stress-induced phase-separated condensates global reduction in synthesis. In contrast, inducible bypass this repression remain cytosol where they recruit available components of machinery ensure rapid synthesis HSP70. Although involved preferential translation mRNA during stress have not been fully elucidated, differences factors between yeast mammals suggest organism-specific mechanisms translation. review, we consider these discuss current knowledge on We extend discussion go beyond cytosolic needs ponder important interplay mitochondria activating accumulation, which becomes vital preserving intercompartmental proteostasis cell survival.

Language: Английский

Citations

0
Integration of omics to explore novel disease pathways in Down syndrome neurodegeneration – focusing on integrated stress response DOI Creative Commons
Beatriz Barros-Santos, Carlos Campos‐Marques, Andreia F. Salvador

et al.

Brain Organoid and Systems Neuroscience Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Thermal adaptation in plants: understanding the dynamics of translation factors and condensates DOI
Julia Lohmann, O. Herzog,

Kristina Rosenzweig

et al.

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(14), P. 4258 - 4273

Published: April 17, 2024

Abstract Plants, as sessile organisms, face the crucial challenge of adjusting growth and development with ever-changing environmental conditions. Protein synthesis is fundamental process that enables all organisms. Since elevated temperature presents a substantial threat to protein stability function, immediate adjustments rates are necessary circumvent accumulation proteotoxic stress ensure survival. This review provides an overview mechanisms control translation under high-temperature by modification components machinery in plants, compares them yeast metazoa. Recent research also suggests important role for cytoplasmic biomolecular condensates, named granules, these processes. Current understanding granules translational regulation molecular processes associated might occur within discussed.

Language: Английский

Citations

2
Isolation and Visualization of Plant Stress Granule-Associated Components via On-Beads Digestion and Co-localization Analysis DOI
Jorge Solís-Miranda,

Rafael Rubio-Ramos,

Santiago Gonzalez-Rodriguez

et al.

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 57 - 66

Published: Jan. 1, 2024

Language: Английский

Citations

1
Stress granules and organelles: Coordinating cellular responses in health and disease DOI Creative Commons
Ying Liu, Yin Li, Peipei Zhang

et al.

Protein & Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Membrane-bound organelles and membraneless (MLOs) coordinate various biological processes within eukaryotic cells. Among these, stress granules (SGs) are significant cytoplasmic MLOs that form in response to cellular stress, exhibiting liquid-like properties alongside stable substructures. SGs interact with diverse organelles, thereby influencing pathways critical both health disease contexts. This review discusses the interplay between explores methodologies employed analyze interactions other MLOs. Furthermore, it highlights pivotal roles play regulating responses pathogenesis of amyotrophic lateral sclerosis. Gaining insights into these is essential for deciphering mechanisms underlying physiological pathological conditions.

Language: Английский

Citations

1