Maternal gut microbiota in the health of mothers and offspring: from the perspective of immunology

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 13, 2024

Due to the physiological alteration during pregnancy, maternal gut microbiota changes following metabolic processes. Recent studies have revealed that is closely associated with immune microenvironment in utero pregnancy and plays a vital role specific complications, including preeclampsia, gestational diabetes, preterm birth recurrent miscarriages. Some other evidence has also shown aberrant increases risk of various diseases offspring, such as allergic neurodevelopmental disorders, through alignment between mother fetus possible intrauterine microbiota. Probiotics high-fiber diet are effective inventions prevent mothers fetuses from diseases. In this review, we summarize development complications health condition future generations perspective immunology, which may provide new therapeutic strategies for management offspring.

Language: Английский

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Gestational diabetes augments group B Streptococcus infection by disrupting maternal immunity and the vaginal microbiota

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 3, 2024

Group B Streptococcus (GBS) is a pervasive perinatal pathogen, yet factors driving GBS dissemination in utero are poorly defined. Gestational diabetes mellitus (GDM), complication marked by dysregulated immunity and maternal microbial dysbiosis, increases risk for disease. Using murine GDM model of colonization transmission, we find that mice display greater subsequently worse neonatal outcomes. Dual-RNA sequencing reveals differential adaptation to the reproductive tract, including putative glycosyltransferase (yfhO), altered host responses. immune disruptions include reduced uterine natural killer cell activation, impaired recruitment placentae, maternofetal cytokines. Lastly, observe distinct vaginal taxa associated with status invasive disease status. Here, show hosts recapitulates several clinical aspects identifies multiple bacterial drivers

Language: Английский

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Identification of glyoxalase A in group B Streptococcus and its contribution to methylglyoxal tolerance and virulence

Infection and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

ABSTRACT Group B Streptococcus (GBS) is a Gram-positive pathobiont that commonly colonizes the gastrointestinal and lower female genital tracts but can cause sepsis pneumonia in newborns leading of neonatal meningitis. Despite resulting disease severity, pathogenesis GBS not completely understood, especially during early phases infection. To investigate factors necessary for bloodstream survival, we performed transposon (Tn) mutant screen our bacteremia infection model using mariner library previously developed by group. We identified significantly underrepresented mutations 623 genes contribute to survival blood, including those encoding known virulence such as capsule, β-hemolysin, inorganic metal ion transport systems. Most have been characterized or studied GBS, gloA gloB, which are homologs involved methylglyoxal (MG) detoxification. MG byproduct glycolysis highly reactive toxic aldehyde elevated immune cells Here, observed sensitivity across multiple isolates confirmed contributes tolerance invasive show specifically presence neutrophils depleting mice abrogates decreased mutant. The requirement glyoxalase pathway suggests detoxification important bacterial host-pathogen interactions. IMPORTANCE A transposon-mutant group mouse revealed be β-hemolysin/cytolysin, homeostasis. Many uncharacterized were also part metabolic breaks down (MG). most ubiquitous breakdown only two-step process ( ) gloB enzymes. made cells. first enzyme pathway, encoded , resistance blood survival. further demonstrate GloA against vitro vivo and, therefore, an factor required

Language: Английский

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Gestational diabetes as a risk factor for GBS maternal rectovaginal colonization: a systematic review and meta-analysis

BMC Pregnancy and Childbirth, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 20, 2024

Abstract Background Maternal rectovaginal colonization by group B Streptococcus (GBS) increases the risk of perinatal GBS disease that can lead to death or long-term neurological impairment. Factors increase carriage are incompletely understood resulting in missed opportunities for detecting risk-based clinical approaches. There is a lacking consensus on whether gestational diabetes mellitus (GDM) factor GBS. This systematic review and meta-analysis aims address current conflicting findings determine GDM should be clinically considered as maternal colonization. Methods Peer-reviewed studies provided prevalence documented vaginal and/or rectal women with without were included this analysis. From study inception October 30, 2023, we identified 6,275 relevant from EMBASE PUBMED which 19 eligible inclusion. Eligible analyzed thoroughly assessed bias modified Newcastle-Ottawa Scale interrogated representativeness comparability cohorts, quality reporting status, potential other metabolic diseases. Results synthesized using STATA 18 random-effects meta-analyses. Studies encompassed 266,706 10 different countries, periods spanning 1981 2020. Meta-analysis revealed associated 16% increased (OR 1.16, CI 1.07–1.26, P = 0.003). We also performed subgroup analyses assess independent effects pregestational vs. carriage. Pregestational (Type 1 Type 2 mellitus) was an 76% (pooled OR 1.76, 1.27–2.45, 0.0008). Conclusions achieved among previously discrepant observations demonstrated significant factors Recognition during decisions about screening intrapartum antibiotic prophylaxis may decrease global burden maternal-perinatal health.

Language: Английский

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Maternal and neonatal outcomes of Group B Streptococcus colonization: a retrospective study

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 21, 2025

Group B Streptococcus (GBS) colonization is one of the major causes severe neonatal infections. The study was intended to identify GBS in pregnant women, explore its potential risk factors, and analyze impact on outcomes for both mothers newborns. A retrospective research carried out women who had undergone screening delivered from June 2020 December 2022. Pregnant between 35 37 weeks gestation using real-time polymerase chain reaction (RT-PCR). clinical characteristics newborns were collected. factors linked maternal adverse neonates assessed chi-square logistic regression analyses. composite included low Apgar scores, pneumonia, hyperbilirubinemia, sepsis, or birth weight. Overall, rate positivity 10.63% (551/5183), 88.4%. Diabetic more likely become colonized with GBS. Our revealed that carriers experienced higher rates fetal distress than non-GBS carriers. Fetal (OR, 1.940; 95% CI, 1.355 2.778, P < 0.001), sepsis 5.063; 2.536–10.109, scores 2.097; 1.184–3.715, = 0.011), pneumonia 1.638; 1.039 2.582, 0.034) hyperbilirubinemia 1.438; 1.080 1.915, 0.013) significantly related colonization. When we used as dependent variable analyzed influencing analysis still an elevated (OR 1.752, 1.389–2.208; 0.001). Diabetes may be a factor Moreover, this study, correlated but not outcomes.

Language: Английский

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Characterization of the Cellular Immune Response to Group B Streptococcal Vaginal Colonization

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Introduction Group B Streptococcus (GBS) asymptomatic colonizes the female genital tract (FGT) but can contribute to adverse pregnancy outcomes including pre-term birth, chorioamnionitis, and neonatal infection. We previously observed that GBS elicits FGT cytokine responses, IL-17, during murine vaginal colonization; yet anti-GBS cellular immune response colonization remained unknown. hypothesized may induce immunity, resulting in clearance. Methods Herein, we utilize depleting antibodies knockout mice performed flow cytometry investigate immunes responses colonization. Results found neutrophils (effectors of IL-17 response) are important for mucosal control as neutrophil depletion promoted increased burdens tissues. Flow cytometric analysis populations vagina, cervix, uterus revealed, however, did not a marked increase CD45+ cells. also Vγ6+ γδ T cells comprise primary source IL-17. Finally, using mice, IL-17-producing Conclusions Taken together, this work characterizes immunity suggests does elicit significant response, which be bacterial directed adaptive outcome. However, certain cells, such ɣδ host defense

Language: Английский

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Gestational diabetes mellitus and group B streptococcus maternal colonization

European Journal of Obstetrics & Gynecology and Reproductive Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Long-term risk of psychiatric disorders in childhood and adolescence following neonatal invasive group B Streptococcus disease—a Danish cohort study

Journal of Infection, Journal Year: 2025, Volume and Issue: unknown, P. 106463 - 106463

Published: March 1, 2025

The long-term risk of child and adolescent psychiatric disorders (PDs) after neonatal invasive group B Streptococcus disease (iGBS) the modifying factors are poorly understood. A population-based matched cohort study, including 1,548 infants with iGBS diagnosed during first 3 months life from 1997 through 2020 follow-up until 2022, based on data Danish national health administrative registers. general population comparison without was randomly sampled 1:10 by sex, child's birth year month, gestational age (n= 15,345). cumulative incidence proportion (CIP) 95% confidence intervals (CIs) any PD 21.1% (95% CI 18.7-23.7) in exposed to (88% sepsis, 12% meningitis) 16.2% 15.5-17.0) cohort. 18-year CIP higher for meningitis compared sepsis. adjusted hazard ratio 1.41 1.23-1.62). early infancy is a factor PDs, especially meningitis. Premature birth, diabetes, low maternal education increased further. Group (Streptococcus Agalactiae) remains leading cause neonatal, associated high mortality neurodevelopmental impairments. Scarce exist regarding following (iGBS), In this we investigated association adolescence adulthood. We which extend premature sex (at birth), socioeconomic position or diabetes were between disorders. Our study found an persisting into iGBS, both Specific elevated included anxiety, obsessive compulsive disorder, autism, attention deficit hyperactivity disorder. further disorders, whereas did not.

Language: Английский

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Preclinical validation of electrospun fibers to achieve vaginal colonization by Lactobacillus crispatus

Frontiers in Bacteriology, Journal Year: 2025, Volume and Issue: 4

Published: April 16, 2025

Introduction Communities of bacteria collectively known as the vaginal microbiota reside in human vagina. Bacterial vaginosis (BV) describes an imbalance this microbiota, affecting more than 25% women worldwide, and is linked to health problems such infertility, cervical cancer, preterm birth. Following antibiotic treatment, BV becomes recurrent many individuals. Lactobacillus crispatus widely believed contribute a healthy microbiome, its therapeutic application has shown promise early clinical trials investigating adjunct therapies for lasting treatment conditions BV. There pressing need platforms that apply biologically active agents probiotic bacteria, vagina with little user effort but effect. Methods Here, we use mouse model investigate functional utility potential harms soft, slow-dissolving fibers made by electrospinning polyethylene oxide (PEO) poly(lactic- co -glycolic acid) (PLGA). Blank electrospun passed quality control checkpoints were administered vaginally murine compared animals receiving mock procedures. Results Fiber administration had no significant effects on mucus glycan markers, epithelial exfoliation, keratinization, tissue edema or neutrophil infiltration. L. crispatus- loaded enabled colonization most one week. Mice -loaded significantly higher measured concentrations lactate washes at 48 hrs pre-colonization washes. Discussion These data provide pre-clinical proof concept can achieve viable delivery metabolically , without eliciting inflammation injury.

Language: Английский

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Online synthesis of crRNA for One-Step RPA-CRISPR-Cas12 assay of Group b Streptococcus

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113745 - 113745

Published: April 1, 2025

Language: Английский

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