The Dual Role of Cellular Senescence in Macrophages: Unveiling the Hidden Driver of Age-Related Inflammation in Kidney Disease DOI Creative Commons
Yibing Wang, Tong Li, Fengyu Wang

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 21(2), P. 632 - 657

Published: Dec. 31, 2024

Aging is a complex biological process that involves the gradual decline of cellular, tissue, and organ functions.In kidney, aging manifests as tubular atrophy, glomerulosclerosis, progressive renal function decline.The critical role senescence-associated macrophage in diseases, particularly kidney increasingly recognized.During this process, macrophages exhibit range pro-damage response to senescent tissues cells, while themselves also significantly influences disease progression, creating bidirectional regulatory between macrophages.To explore mechanism, review will elucidate origin, characteristic, phenotype, secretory phenotype (SASP), extracellular vesicles from senescence cell-engulfment suppression (SCES), context disease.Additionally, it discuss characteristics macrophage, such common markers, changes autophagy, metabolism, gene regulation, phagocytosis, antigen presentation, exosome secretion, along with their physiological pathological impacts on tissue cells.Furthermore, exploring therapies drugs modulate or eliminate cells may help slow progression damage.

Language: Английский

Role of macrophage in intervertebral disc degeneration DOI Creative Commons

Yiming Dou,

Yiming Zhang, Yang Liu

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1
Comparative evaluation of cellular senescence in naturally aged and stress-induced murine macrophages for identifying optimum senescent macrophage study systems DOI
Mohammed Danish, Bhawna Diwan,

Amit Kumar

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 15, 2025

Language: Английский

Citations

0
N6‐Methyladenosine‐Modified circSMAD4 Prevents Lumbar Instability Induced Cartilage Endplate Ossification DOI Creative Commons
Hanwen Li, Yingchuang Tang,

Sihan Hu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Lumbar instability causes cartilage endplate ossification and intervertebral disc degeneration. In this study, it is determined that circSMAD4, a Yap1-related circRNA, stably downregulated under abnormal stress. vitro, circSMAD4 knockdown resulted in Yap1 mRNA degradation, whereas overexpression increased expression nuclear translocation. Hence, the stabilization of essential for maintaining homeostasis Furthermore, transcriptome sequencing mass spectrometry analysis revealed METTL14-mediated N6-methyladenosine (m6A) modification can stabilize expression. Moreover, shown to regulate through m6A reader IGF2BP1. The IGF2BP1 functions translocate into nucleus, which protects chondrocytes from Finally, local injection an AAV5-containing plasmid successfully rescued LSI-induced degeneration, wasn't observed knockout mice. These findings suggest m6A-modified translocation, thus preventing degeneration may become potential therapeutic tool managing instability-induced

Language: Английский

Citations

0
Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination DOI Creative Commons
Zeeba Manavi, George S. Melchor,

Meghan R Bullard

et al.

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 7, 2025

Language: Английский

Citations

0
Implications of Circadian Disruption on Intervertebral Disc Degeneration: The Mediating Role of Sympathetic Nervous System DOI

Zongmian Song,

Miaoheng Yan,

Shuo Zhang

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102633 - 102633

Published: Dec. 1, 2024

Language: Английский

Citations

1
Slit3 by PTH-Induced Osteoblast Secretion Repels Sensory Innervation in Spine Porous Endplates to Relieve Low Back Pain DOI Creative Commons
Janet L. Crane, Weixin Zhang,

Arryn Otte

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 31, 2024

Abstract During aging, the spine undergoes degenerative changes, particularly with vertebral endplate bone expansion and sclerosis, that is associated nonspecific low back pain (LBP). We reported parathyroid hormone (PTH) treatment could reduce sclerosis improve behaviors in SM/J young lumbar instability (LSI) mice. Aberrant innervation noted body during spinal degeneration was reduced PTH aging LSI mice as quantified by PGP9.5+ CGRP+ nerve fibers, well CGRP expression dorsal root ganglia (DRG). The neuronal repulsion factor Slit3 significantly increased response to mediated transcriptional FoxA2. type1 receptor (PPR) deletion osteoblasts prevented PTH-reduction of porosity improvement behavior tests, whereas PPR chondrocytes continued respond PTH. Altogether, stimulates repel sensory provides symptomatic relief LBP degeneration.

Language: Английский

Citations

0
Single-cell RNA sequencing reveals the differentiation and regulation of endplate cells in human intervertebral disc degeneration DOI Creative Commons

Chenhao Shi,

Yonggang Fan,

Xiusheng Huang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 13, 2024

Low back pain (LBP) is largely attributed to intervertebral disc degeneration (IVDD), of which the endplate changes are an important component. However, alterations in cell fate and properties within endplates during remain unknown. Here, we firstly performed single-cell RNA-sequencing analysis (scRNA-seq) cells focusing on degenerative human endplates. By unsupervised clustering 8,534 based gene expression, identified nine distinct types. We employed Gene ontology (GO) analysis, Kyoto Encyclopedia Genes Genomes (KEGG) pathways regulatory network inference (SCENIC) determine enriched transcriptional activities across seven chondrocyte subpopulations. Furthermore, two fates differentiation were found by trajectory one was inflammation-related genes, other related extracellular matrix (ECM). Additionally, intercellular interactions macrophages (MA) chondrocytes, T cells/natural killer (T/NK) chondrocytes examined ligand-receptor pairs showing regulative function FN1 from MA CD74 T/NK degeneration. Overall, our findings provide novel perspectives at level a whole-transcriptome size.

Language: Английский

Citations

0
Decellularized Extracellular Matrix and Polyurethane Vascular Grafts Have Positive Effects on the Inflammatory and Pro‐Thrombotic State of Aged Endothelial Cells DOI Creative Commons

Sophie J. Specht,

Sabrina Rohringer,

Pia Hager

et al.

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

ABSTRACT In vitro assessment of small‐diameter synthetic vascular grafts usually uses standard cell culture conditions with early‐passage cells. However, these conduits are mainly implanted in elderly patients and subject to complex cellular interactions influenced by age inflammation. Understanding factors is central the development tailored specific needs patients. this study, effects aged endothelial cells subjected pro‐ anti‐inflammatory agents cultivated on a newly developed biodegradable electrospun thermoplastic polyurethane/poly(urethane‐urea) blend (TPU/TPUU), clinically available expanded polytetrafluorethylene (ePTFE), decellularized extracellular matrix (dECM) were investigated. Young exposed characterized morphology, migration capacity, gene expression. addition, seeded onto various graft materials examined microscopically alongside expression analyses. When pro‐inflammatory cytokines, young demonstrated signs activation. Cells ePTFE showed reduced attachment increased genes compared other materials. dECM TPU/TPUU substrates provided better support for endothelialization under inflammatory ePTFE. Moreover, positive reducing pro‐thrombotic Our results thus emphasize importance developing new as an alternative used

Language: Английский

Citations

0
SenMayo transcriptomic senescence panel highlights glial cells in the ageing mouse and human retina DOI Creative Commons
Samyuktha Suresh, Gayathri Karthik, John F. Ouyang

et al.

npj Aging, Journal Year: 2024, Volume and Issue: 10(1)

Published: Nov. 30, 2024

There is a growing need to better characterise senescent cells in the CNS and retina. The recently published SenMayo gene panel was developed identify transcriptomic signatures of senescence across multiple organ systems, but retina not included. While other approaches have identified retina, these largely focused on experimental models young animals. We therefore conducted detailed single-cell RNA-seq analysis cell populations different aged mice compared with five comprehensive human mouse brain transcriptome datasets. Transcriptomic were most apparent retinal glial cells, IL4, 13 10 AP1 pathway being prominent markers involved. Similar levels transcriptional observed glia old mice, whereas showed significantly increased enrichment scores advancing age.

Language: Английский

Citations

0
The Dual Role of Cellular Senescence in Macrophages: Unveiling the Hidden Driver of Age-Related Inflammation in Kidney Disease DOI Creative Commons
Yibing Wang, Tong Li, Fengyu Wang

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 21(2), P. 632 - 657

Published: Dec. 31, 2024

Aging is a complex biological process that involves the gradual decline of cellular, tissue, and organ functions.In kidney, aging manifests as tubular atrophy, glomerulosclerosis, progressive renal function decline.The critical role senescence-associated macrophage in diseases, particularly kidney increasingly recognized.During this process, macrophages exhibit range pro-damage response to senescent tissues cells, while themselves also significantly influences disease progression, creating bidirectional regulatory between macrophages.To explore mechanism, review will elucidate origin, characteristic, phenotype, secretory phenotype (SASP), extracellular vesicles from senescence cell-engulfment suppression (SCES), context disease.Additionally, it discuss characteristics macrophage, such common markers, changes autophagy, metabolism, gene regulation, phagocytosis, antigen presentation, exosome secretion, along with their physiological pathological impacts on tissue cells.Furthermore, exploring therapies drugs modulate or eliminate cells may help slow progression damage.

Language: Английский

Citations

0