Photothermal Energy Conversion by Heme in Cytochrome c552: A Heme Protein-Based Molecular Heater DOI

Taro Watase,

Misao Mizuno, Yasuhisa Mizutani

et al.

The Journal of Physical Chemistry C, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

The development of efficient photothermal converters is essential for thermal microscopy and the manipulation materials. Photoexcitation heme in proteins its subsequent nonradiative transition convert light energy into energy. converted released to surroundings, resulting heating solvent water. Here, we examined heat release by cytochrome c552 from Thermus thermophilus following conversion group protein. Upon irradiation with 532 nm light, Raman bands associated OH stretching mode water changed solutions but not protein-free buffer solutions. observed spectral changes closely resembled temperature-dependent were attributed a temperature increase due rise averaged over volume irradiated 125 mW was 5.1 K, which exceeds threshold activate thermosensitive cells. We discuss protein based on diffusion model excellent potential applications protein-based molecular heaters living

Language: Английский

Temperature response of defect photoluminescence in locally functionalized single-walled carbon nanotubes DOI Creative Commons
Ryo Hamano,

Yoshiaki Niidome,

Naoki Tanaka

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(6), P. 4137 - 4148

Published: Jan. 1, 2025

Near-infrared emission from low-density defects in single-walled carbon nanotubes (SWCNTs) functions as a highly sensitive thermometer, with its sensitivity varying depending on factors such the defect structure and length of SWCNTs.

Language: Английский

Citations

1

Neural differentiation in perspective: mitochondria as early programmers DOI Creative Commons
Ramin Hamidi Farahani

Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 18

Published: Jan. 8, 2025

Neural differentiation during development of the nervous system has been extensively studied for decades. These efforts have culminated in generation a detailed map developmental events that appear to be associated with emergence committed cells system. In this review landscape neural is revisited by focusing on abiotic signals play role induction differentiation. Evidence presented regarding chimeric whereby generated mitochondria orchestrate early This stage, characterised mitochondrial hyperactivity, turn triggers late stage reprogramming activity biotic signals.

Language: Английский

Citations

0

Thyroid Hormones and Brain Development: A Focus on the Role of Mitochondria as Regulators of Developmental Time DOI Creative Commons
Filip Vujovic, Ramin Hamidi Farahani

Cells, Journal Year: 2025, Volume and Issue: 14(3), P. 150 - 150

Published: Jan. 21, 2025

Thyroid hormones (THs) regulate metabolism in a homeostatic state an adult organism. During the prenatal period, prior to establishment of mechanisms, THs assume additional functions as key regulators brain development. Here, we focus on reviewing role orchestrating cellular dynamics developing brain. The evidence from reviewed scientific literature suggests that developmental roles are predominantly mediated by non-genomic mitochondrial effects due attenuation genomic antagonise impacts. We argue function TH signalling during development is orchestrate tempo self-organisation neural progenitor cells. Further, provided major neurodevelopmental consequences hypothyroidism stem altered self-organisation.

Language: Английский

Citations

0

Light-responsible ECM-mimetic scaffolds for neural differentiation. Intracellular versus extracellular photothermal stimulation DOI Creative Commons
O. Yu. Antonova, O. Yu. Kochetkova,

Maxim Tailakov

et al.

Smart Materials in Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0

Nanobiodevices and quantum life science for future healthcare DOI Open Access
Taisuke Shimada, Takao Yasui,

Hiroshi Yukawa

et al.

Analytical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Maintaining healthy life in old age (healthy aging) is an important social challenge. An effective approach to realizing aging involves providing personalized healthcare through disease prevention, early diagnostics, and optimal treatments. Nanobiodevices quantum sciences have tremendous potential revolutionize current techniques for treatment via the efficient analyses of biomolecules, bioparticles, pathogens, cells. In this review, we outline our research on nanobiodevices future healthcare, including cancer pathogen detection, vivo imaging-guided therapy, intracellular sensing stem cell quality checks.

Language: Английский

Citations

0

Imaging‐Guided Microscale Photothermal Stereolithography Bioprinting DOI Creative Commons
Jingyu Sun,

Tianqi Fang,

Yuze Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Stereolithography bioprinting relies heavily on costly photoinitiators for polymerization, limiting its potential further technical advancement to meet growing needs in tissue engineering and regenerative medicine. Thermal initiators, contrast, are low cost, rapid growth of the photothermal conversion field offers a wide range materials tools convert light into heat. However, high-resolution stereolithography remains unattainable due difficulty confining heat an aqueous environment. Here, this challenge has been fully addressed by establishing imaging-guided microscale (ImPSB). This technique is achieved through building novel system that provides depth-resolved visualization printing dynamics, creating unique initiator second near-infrared window, developing new bioink seeing controlling gelation process. ImPSB achieves resolution ≈47 µm generates smooth lines arbitrarily designed shapes with cross-sectional diameter as small ≈104 µm, representing unprecedented scale from stereolithography. Its cellular biocompatibility both bioscaffold cell-laden hydrogel demonstrated, feasibility transdermal also shown. work sets path where vast resources can be utilized.

Language: Английский

Citations

0

Fluorescent Thermometers Based on Carbon Quantum Dots with Various Detection Modes for Intracellular Temperature Measurement DOI

Yasumasa Kato,

Yukiho Shimazaki,

Shunsuke Chuma

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

We report that carbon quantum dots (CQDs) synthesized via a hydrothermal process using anthraquinone derivatives and l-cysteine provide versatile detection modes, making them suitable for various experimental setups. By modification of the precursor structures, these CQDs can function as different types fluorescent nanothermometers, including those based on fluorescence intensity, ratiometrics, lifetime. Notably, lifetime-based demonstrate robust performance under wide range conditions, variations in pH ionic strength. The exhibit low cytotoxicity high cellular uptake efficiency, enabling wash-free imaging precise temperature measurements at single-cell level. Furthermore, we successfully measured changes associated with biochemical reactions, increase induced by mitochondrial depolarization. In addition, could be cross-verified their intensity. These findings underscore potential minimally invasive tools nanoscale thermometry live cells.

Language: Английский

Citations

0

Exploring the Frontiers of Cell Temperature Measurement and Thermogenesis DOI Creative Commons
Hanliang Zhu, Haotian Xu, Yue Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Abstract The precise measurement of cell temperature and an in‐depth understanding thermogenic processes are critical in unraveling the complexities cellular metabolism its implications for health disease. This review focuses on mechanisms local generation within cells array methods developed accurate assessment. contact noncontact techniques introduced, including infrared thermography, fluorescence thermometry, other innovative approaches to localized measurement. role thermogenesis metabolism, highlighting integral function regulation processes, environmental adaptation, dysregulation diseases such as metabolic disorders cancer further discussed. challenges limitations this field critically analyzed while technological advancements future directions proposed overcome these barriers. aims provide a consolidated resource current methodologies, stimulate discussion challenges, inspire innovations study thermodynamics.

Language: Английский

Citations

1

High-speed Intracellular Temperature Mapping Reveals the Existence of Non-Conductive Dissipation of Energy by Heating DOI Creative Commons

Masaharu Takarada,

Ryo Shirakashi, Masahiro Takinoue

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Abstract Recently, intracellular thermometry has revealed temperature variations within cells. Although the biological significance of change is recognized, physical principles remain a mystery. Here, we investigate heat transfer through mapping using fluorescent polymeric thermometer and high-speed fluorescence lifetime imaging microscopy. Through infrared laser irradiation assisted heating, track changes in distribution to examine mechanism dissipation relation conduction. Continuous heating provokes significantly slower (second order) relaxation averaged single cells compared liposomes comparable size, affected by structures molecules. Furthermore, reveal existence independent Our results may explain mechanisms variation that are unresolved based on our current understandings, providing first step toward unraveling thermodynamics under non-equilibrium conditions.

Language: Английский

Citations

0

Cellular Thermal Biology Using Fluorescent Nanothermometers DOI
Kohki Okabe, Shingo Sotoma, Yoshie Harada

et al.

Advances in experimental medicine and biology, Journal Year: 2024, Volume and Issue: unknown, P. 97 - 108

Published: Jan. 1, 2024

Language: Английский

Citations

0