Journal of Extracellular Vesicles,
Journal Year:
2025,
Volume and Issue:
14(4)
Published: April 1, 2025
ABSTRACT
Yersinia
pestis
is
the
etiologic
agent
of
plague.
A
hallmark
plague
subversion
host
immune
response
by
disrupting
signalling
pathways
required
for
inflammation.
This
non‐inflammatory
environment
permits
bacterial
colonization
and
has
been
shown
to
be
essential
disease
manifestation.
Previous
work
that
Y.
inhibits
phagocytosis
degranulation
neutrophils.
Manipulation
these
key
vesicular
trafficking
suggests
influences
extracellular
vesicle
(EV)
secretion,
cargo
selection,
and/or
maturation.
Our
goals
were
define
EV
population
produced
neutrophils
in
determine
how
vesicles
might
influence
Towards
goals,
EVs
isolated
from
human
infected
with
or
a
mutant
lacking
effector
proteins
known
manipulate
cell
signalling.
Mass
spectrometry
data
revealed
cargoes
packaged
cells
enriched
antimicrobial
cytotoxic
proteins,
contents
which
differed
uninfected
cells.
Further,
lacked
inflammatory
properties
observed
those
responding
mutant.
Together,
demonstrate
actively
production
neutrophils,
likely
contributing
evasion.
ABSTRACT
Background
The
recent
consensus
statement
redefined
steatotic
liver
diseases.
Metabolic
dysfunction‐associated
disease
(MASLD)
and
metabolic
dysfunction
alcohol‐related
(MetALD)
now
represent
distinct
entities.
However,
biomarkers
that
differentiate
MASLD
MetALD
remain
largely
unknown.
Aims
To
identify
lipidomic
with
discriminatory
potential
for
distinguishing
from
MASLD.
Methods
Using
the
UK
Biobank
dataset,
40,534
people
available
MRI
scans
were
analysed.
A
total
of,
11,217
cases
a
proton
density
fat
fraction
(PDFF)
≥
5%
identified
as
having
disease.
Among
these,
profiles
obtained
5539
462
cases.
250
plasma
metabolomic
parameters
Mendelian
randomisation
(MR)
analysis
was
used
to
confirm
association
between
alcohol
consumption
biomarkers.
Results
When
comparing
top
30
differentially
expressed
predicting
compared
MASLD,
majority
related
HDL
significantly
overrepresented
at
both
analysed
time
points.
five
metabolites
were:
acetoacetate,
3‐hydroxybutyrate,
phospholipids
in
Large
HDL,
concentration
of
large
particles,
free
cholesterol
HDL.
sensitivity
revealed
similar
associations,
suggesting
signature
is
stable
over
time.
Additionally,
MR
further
confirmed
associated
increased
levels
HDL‐related
metabolites.
Conclusion
Our
findings
indicate
HDL‐centric
markers,
particularly
those
within
larger
medium
subfraction,
may
Further
longitudinal
experimental
studies
are
warranted
validate
these
assess
their
clinical
implications.
BBA Advances,
Journal Year:
2025,
Volume and Issue:
7, P. 100140 - 100140
Published: Jan. 1, 2025
High-grade
serous
ovarian
carcinoma
(HGSOC)
is
a
deadly
subtype
of
cancer
(OC),
often
diagnosed
at
late
stages
due
to
nonspecific
symptoms
and
lack
effective
markers
for
early
detection.
Aberrant
protein
N-linked
glycosylation
has
been
reported
in
HGSOC,
holding
potential
improving
the
diagnosis
prognosis
affected
patients.
Building
on
our
recent
observation
documenting
that
HGSOC-derived
extracellular
vesicles
(EVs)
exhibit
aberrant
expression
patterns,
we
here
explore
N-glycosylation
displayed
by
EVs
isolated
from
HGSOC
cell
lines
patient
ascites
relative
those
matching
controls
unveil
candidate
HGSOC.
Comparative
glycoproteomics
small
(sEVs,
<200
nm)
medium/large
(m/lEVs,
>200
non-cancerous
revealed
lower
overall
EV
proteins
decreased
oligosaccharyltransferase
(OST)
subunits
compared
lines.
Increased
α2,6-sialylation
was
also
observed
m/lEVs
lectin
blotting,
which
correlated
with
increased
gene
ST6GAL1
ST3GAL3/4
normal
ovary
tissues.
Our
study
provides
insights
into
glycoproteome
underlying
changes
machinery
tissues,
opening
new
avenues
discovery
novel
against
aBIOTECH,
Journal Year:
2025,
Volume and Issue:
6(1), P. 116 - 132
Published: Jan. 23, 2025
Abstract
Plant
metabolites
are
crucial
for
the
growth,
development,
environmental
adaptation,
and
nutritional
quality
of
plants.
metabolomics,
a
key
branch
systems
biology,
involves
comprehensive
analysis
interpretation
composition,
variation,
functions
these
metabolites.
Advances
in
technology
have
transformed
plant
metabolomics
into
sophisticated
process
involving
sample
collection,
metabolite
extraction,
high-throughput
analysis,
data
processing,
multidimensional
statistical
analysis.
In
today’s
era
big
data,
field
is
witnessing
an
explosion
acquisition,
offering
insight
complexity
dynamics
metabolism.
Moreover,
multiple
omics
strategies
can
be
integrated
to
reveal
interactions
regulatory
networks
across
different
molecular
levels,
deepening
our
understanding
biological
processes.
this
review,
we
highlight
recent
advances
challenges
emphasizing
roles
technique
improving
crop
varieties,
enhancing
value,
increasing
stress
resistance.
We
also
explore
scientific
foundations
its
applications
medicine,
ecological
conservation.
The
term
protein
corona
(PC)
indicates
proteins
adsorbed
onto
the
surface
of
nanostructures
exposed
to
biological
media
such
as
blood
or
serum.
analysis
composition,
evolution,
and
effect
PC
complexed
with
nanomaterials
gained
attention
in
recent
years
due
importance
these
parameters
determining
fate
nanostructures.
In
particular,
represents
first
component
a
nanomaterial
interfacing
structures,
dictating
nanoparticle
internalization,
immune
response,
bioavailability,
even
toxicity.
Polydopamine
nanoparticles
(PDNPs),
obtained
through
polymerization
dopamine,
are
"smart"
materials
characterized
by
high
biocompatibility,
antioxidant
capacities,
tunability
reactivity,
biodegradability,
ability
act
photothermal
conversion
agents
when
irradiated
near-infrared
(NIR)
light
source.
Despite
many
interesting
applications
PDNPs
currently
described
scientific
literature,
there
is
still
no
comprehensive
phenomenon
formation
consequent
exposure
media.
Moreover,
date,
investigation
effects
irradiation
photothermally
active
on
composition
evolution
associated
has
been
extremely
limited.
With
this
work,
we
aim
provide
for
time
an
PDNPs,
before
after
NIR
stimulation.
We
formed
following
human
plasma
analyzed
several
overall
quantity,
PDNP
size,
presence
functionalization,
time,
laser,
demonstrating
that
play
pivotal
role
resulting
composition.
Eventually,
showed
have
significantly
different
properties
respect
bare
showing
higher
internalization
rates
glioblastoma
cells,
absorption
value,
enhanced
abilities.
Experimental Cell Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 114452 - 114452
Published: Feb. 1, 2025
Characterizing
the
initial
stages
of
oncogenic
transformation
allows
identification
tumor-promoting
processes
before
inherent
clonal
selection
aggressive
clones.
Here,
we
used
global
proteomics,
genetic
cell
tracing,
and
immunofluorescence
to
dynamically
map
very
early
cancer
initiation
in
a
mouse
model
bladder
cancer.
We
observed
rapid
incremental
proteome
dysregulation,
with
changes
energy
metabolism,
proliferation
immune
signatures
dominating
landscape.
The
lipid
metabolism
were
immediate
defined
by
an
increase
fatty
acid
transport,
followed
activation
Alongside
signature
also
mapped
clear
cycle-related
pathways
proliferation.
Proliferation
was
mainly
restricted
basal
epithelial
layer
rapidly
leading
urothelium
thickening,
despite
progressive
loss
superficial
layer.
Moreover,
tilt
balance
towards
increased
glucose
probably
characterizing
cells
tumor
microenvironment.
All
persistent,
being
retained
sometimes
intensified
or
diversified
along
timeline.
this
pilot
suggest
these
as
potentially
targetable
drivers
future
neoplastic
transformations
bladder.
Foods,
Journal Year:
2025,
Volume and Issue:
14(5), P. 716 - 716
Published: Feb. 20, 2025
This
study
aimed
to
investigate
the
flavor
formation
and
meat
quality
differences
among
different
beef
cuts
in
Chinese
Wagyu
cattle.
The
metabolites
gene
expression
profiles
of
chuck,
neck,
rump,
tenderloin,
longissimus
lumborum
were
analyzed.
results
revealed
that
a
total
240
volatile
organic
compounds
779
lipid
molecules
detected
cuts,
with
hydrocarbons
(accounting
for
29.71%)
triglycerides
(representing
41.21%)
emerging
as
most
prominent
compounds,
respectively.
sensory-directed
analysis
highlighted
significance
sweet
fruity
aroma
which
contributed
distinct
cuts.
Additionally,
60
key
molecular
markers,
including
FA(18:1),
PC(40:5),
TG(18:0_16:1_18:1),
TG(36:0_18:1),
etc.,
identified
playing
crucial
roles
generation
essential
across
five
Integrative
multi-omics
data
pinpointed
cluster
differentially
expressed
genes
(e.g.,
DLD,
ACADM,
PCCA,
SCD),
involved
regulation
valine,
leucine,
isoleucine
degradation
pathways
metabolism.
Taken
together,
this
has
candidate
influencing
providing
valuable
resource
breeding
high-quality
traits
Molecular Plant Pathology,
Journal Year:
2025,
Volume and Issue:
26(2)
Published: Feb. 1, 2025
ABSTRACT
Plant
viruses
pose
a
significant
threat
to
global
agriculture,
leading
substantial
crop
losses
that
jeopardise
food
security
and
disrupt
ecosystem
stability.
These
viral
infections
often
reprogramme
plant
metabolism,
compromising
key
pathways
critical
for
growth
defence.
For
instance,
by
cucumber
mosaic
virus
alter
amino
acid
secondary
metabolite
biosynthesis,
including
flavonoid
phenylpropanoid
pathways,
thereby
weakening
defences.
Similarly,
tomato
bushy
stunt
disrupts
lipid
metabolism
altering
the
synthesis
accumulation
of
sterols
phospholipids,
which
are
essential
replication
compromise
membrane
integrity.
Recent
advancements
in
gene‐editing
technologies,
such
as
CRISPR/Cas9,
metabolomics
offer
innovative
strategies
mitigate
these
impacts.
Precise
genetic
modifications
can
restore
or
optimise
disrupted
metabolic
enhancing
resilience
infections.
Metabolomics
further
aids
identifying
biomarkers
linked
resistance,
guiding
breeding
programmes
aimed
at
developing
virus‐resistant
plants.
By
reducing
susceptibility
crops
infections,
approaches
hold
potential
reduce
dependence
on
chemical
pesticides,
increase
yields
promote
sustainable
agricultural
practices.
Future
research
should
focus
expanding
our
understanding
virus–host
interactions
molecular
level
while
exploring
long‐term
ecological
impacts
Interdisciplinary
integrating
multi‐omics
technologies
management
will
be
addressing
challenges
posed
ensuring
