Inflammation,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 19, 2024
5-aminolevulinic
acid
(5-ALA)
is
an
amino
essential
for
the
synthesis
of
heme,
which
important
various
cellular
functions,
including
mitochondrial
electron
transport
chain.
We
previously
established
heterozygous
knockout
mice
(Alas1+/−)
5-ALA
synthase
1
(ALAS1),
rate-limiting
enzyme
synthesis,
and
reported
that
developed
non-obese
insulin-resistant
diabetes.
In
present
study,
we
used
these
to
analyze
role
in
immune
system.
Using
a
lipopolysaccharide
(LPS)-induced
septic
shock
model,
Alas1+/−
showed
reduced
mortality
compared
wild-type
(WT)
mice.
this
model
experiment,
plasma
concentration
inflammatory
cytokines
such
as
tumor
necrosis
factor
α
(TNFα)
interleukin-6
(IL-6),
chemokine
monocyte
chemoattractant
protein-1
(MCP1)
decreased
WT
mice,
cell
infiltration
into
peritoneal
cavity
was
also
decreased.
ex
vivo
experiments,
exogenous
pretreatment
enhanced
LPS-induced
TNFα
IL-6
production
from
peripheral
blood
leukocytes
Additionally,
activation
cytokine
genes
innate
cells.
Interestingly,
phagocytosis
reactive
oxygen
species
(ROS)
producing
abilities
neutrophils
were
clearly
hampered
but
after
2
weeks
administration
both
significantly
recovered
up
level
These
results
reveal
function
Because
can
be
supplemented
orally,
it
has
potential
drug
restore
function.
Drug Testing and Analysis,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 6, 2025
ABSTRACT
The
natural
extracellular
hemoglobin
of
the
lugworm
Arenicola
marina
(AmHb)
has
many
interesting
characteristics:
It
carries
40
times
more
oxygen
than
human
hemoglobin;
anti‐inflammatory,
antibacterial,
and
antioxidant
properties;
is
250
smaller
a
red
blood
cell.
nontoxic
nonimmunogenic.
thus
very
promising
hemoglobin‐based
carrier.
AmHb
extracted
purified
in
GMP
conditions
to
produce
therapeutic
molecule,
called
M101.
used
various
forms
(liquid,
hydrogel,
lyophilized)
respond
different
situations
hypoxia
healthcare
field,
such
as
organ
preservation
prior
transplantation,
wound
burn
healing,
periodontitis,
sickle
cell
disease,
transfusions,
particularly
emergency
situations.
Given
these
remarkable
transport
capacities,
M101
could
be
misused
for
doping
purposes.
This
article
presents
current
future
developments
this
molecule.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 27, 2025
Tissue
engineering
aims
to
repair
damaged
tissues
with
physiological
functions
recovery.
Although
several
therapeutic
strategies
are
there
for
tissue
regeneration,
the
functional
recovery
of
regenerated
still
poses
significant
challenges
due
lack
concerns
innervation.
Design
rationale
multifunctional
biomaterials
both
tissue-induction
and
neural
induction
activities
shows
great
potential
regeneration.
Recently,
research
application
inorganic
attracts
increasing
attention
in
innervated
multi-tissue
such
as
central
nerves,
bone,
skin,
because
its
superior
tunable
chemical
composition,
topographical
structures,
physiochemical
properties.
More
importantly,
easily
combined
other
organic
materials,
biological
factors,
external
stimuli
enhance
their
effects.
This
review
presents
a
comprehensive
overview
recent
advancements
It
begins
introducing
classification
properties
typical
design
inorganic-based
material
composites.
Then,
progresses
regenerating
various
nerves
nerve-innervated
systematically
reviewed.
Finally,
existing
future
perspectives
proposed.
may
pave
way
direction
offers
new
strategy
regeneration
combination
Gut,
Journal Year:
2025,
Volume and Issue:
unknown, P. gutjnl - 333885
Published: March 3, 2025
Background
To
treat
liver
failure,
three-dimensional
(3D)
bioprinting
is
a
promising
technology
used
to
construct
hepatic
tissue
models.
However,
current
research
on
of
models
primarily
relies
conventional
single-cell-based
bioprinting,
where
individual
functional
hepatocytes
are
dispersed
and
isolated
within
hydrogels,
leading
insufficient
treatment
outcomes
due
inadequate
cell
functionality.
Objective
Here,
we
aim
bioprint
model
using
hepatocyte
organoids
(HOs)
evaluate
its
liver-specific
functions
in
vitro
vivo
.
Design
Human
chemically
induced
pluripotent
stem
cells
(hCiPSCs)
were
as
robust
non-genome-integrative
source
produce
highly
viable
HOs
(hCiPSC-HOs).
An
oxygen-permeable
microwell
device
was
enhance
oxygen
supply,
ensuring
high
viability
promoting
hCiPSC-HOs
maturation.
maintain
the
long-term
biofunction
hCiPSC-HOs,
spheroid-based
employed
(3DP-HOs).
3DP-HOs
intraperitoneally
implanted
mice
with
failure.
Results
demonstrated
enhanced
when
compared
fabricated
exhibited
gene
profiles
closely
resembling
while
maintaining
Moreover,
implantation
significantly
improved
survival
CCl
4
-induced
acute-on-chronic
failure
also
Fah−/−
reduced
injury,
inflammation
fibrosis
indices
regeneration
expression.
Conclusion
Our
bioprinted
exhibits
remarkable
therapeutic
efficacy
for
holds
great
potential
clinical
field
regenerative
medicine.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 28, 2024
Periodontal
disease
is
a
multifactorial,
bacterially
induced
inflammatory
disorder
characterized
by
progressive
destruction
of
periodontal
tissues.
Additionally,
diabetes
mellitus
exacerbates
periodontitis,
resulting
in
expedited
resorption
bone.
However,
methods
such
as
mechanical
debridement,
anti-inflammatory
medications,
and
surgical
approaches
often
fail
to
eradicate
local
infections
inflammation,
complicating
the
reconstruction
tissue
structures.
Consequently,
there
an
urgent
need
devise
novel
strategy
for
managing
diabetic
conditions.
Here,
multifunctional
controlled-release
drug
delivery
system
(GOE1)
developed
encapsulating
self-assembled
nanoparticles
(consisting
chlorhexidine
acetate
epigallocatechin-3-gallate)
into
hydrogel
matrix
composed
gelatin
methacryloyl
oxidized
hyaluronic
acid.
In
vitro
experiments
demonstrate
that
GOE1
possesses
good
antimicrobial,
antioxidant
properties,
transgenic
sequence
genomics
further
illustrates
IL-17-producing
RAW
264.7
macrophages
are
critical
mediating
M1/M2
macrophage
transition
provide
favorable
immune
microenvironment.
addition,
vivo
reveal
significantly
ameliorates
inflammation
reduces
loss
alveolar
bone
reducing
infiltration
collagen
destruction.
Overall,
offers
promising
therapeutic
option
periodontitis.
Journal of Biomedical Materials Research Part B Applied Biomaterials,
Journal Year:
2025,
Volume and Issue:
113(2)
Published: Jan. 22, 2025
ABSTRACT
Intervertebral
disc
degeneration
(IDD)
is
one
of
the
leading
causes
chronic
pain
and
disability,
traditional
treatment
methods
often
struggle
to
restore
its
complex
biomechanical
properties.
This
article
explores
innovative
application
self‐healing
hydrogels
in
IDD,
offering
new
hope
for
repair
due
their
exceptional
self‐repair
capabilities
adaptability.
As
a
key
support
structure
human
body,
intervertebral
discs
are
damaged
by
trauma
or
degenerative
changes.
Self‐healing
not
only
mimic
mechanical
properties
natural
but
also
when
damaged,
thereby
maintaining
stable
functionality.
reviews
mechanisms
design
strategies
and,
first
time,
outlines
potential
IDD.
Furthermore,
looks
forward
future
developments
field,
including
intelligent
material
design,
multifunctional
integration,
encapsulation
release
bioactive
molecules,
combinations
with
tissue
engineering
stem
cell
therapy,
perspectives
IDD
treatment.
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(2), P. 201 - 201
Published: Jan. 31, 2025
Acute
and
chronic
coronary
artery
disease
(CAD)
are
interconnected,
representing
two
facets
of
the
same
condition.
Chronic
CAD
exhibits
a
dynamic
nature,
manifesting
as
stable
or
acute
ischemia,
both.
Myocardial
ischemia
can
be
transient
reversible.
The
genesis
involves
diverse
anatomical
functional
mechanisms,
including
endothelial
dysfunction,
arteriolar
remodeling,
capillary
rarefaction,
perivascular
fibrosis,
though
no
single
factor
explains
its
heterogeneity.
is
often
but
may
present
symptomatic
asymptomatic
(e.g.,
in
diabetes)
affect
various
compartments
(epicardial
microcirculation).
This
complexity
necessitates
reappraisal
our
approach,
pathophysiological
mechanisms
vary
overlap.
A
comprehensive
exploration
these
using
advanced
diagnostic
techniques
aid
identifying
processes
underlying
CAD.
obstructive
non-obstructive,
unstable,
underscoring
diversity.
primary
source
lies
arterial
wall,
emphasizing
need
for
research
on
components,
such
endothelium
vascular
smooth
muscle
cells,
factors
disrupting
homeostasis.
Shifting
focus
from
luminal
status
to
wall
provide
insights
into
atheromatous
plaques,
enabling
earlier
interventions
prevent
their
development
progression.
Climacteric,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 6
Published: Feb. 4, 2025
Objective
This
study
aimed
to
evaluate
the
effect
of
vaginal
oxygen
and
hyaluronic
acid
on
lower
urinary
tract
symptoms
(LUTS)
in
breast
cancer
survivors.
Dentistry Journal,
Journal Year:
2025,
Volume and Issue:
13(2), P. 77 - 77
Published: Feb. 10, 2025
Background:
Several
therapies
involving
the
use
of
oxygen
have
been
developed;
however,
literature
to
date
has
poorly
addressed
effects
Topical
Oxygen
Therapy
(TOT)
on
oral
tissues.
The
aim
present
pilot
study
was
histologically
evaluate
response
TOT
in
epithelial-connective
samples
harvested
from
palate.
Methods:
In
eight
patients
selected
for
a
free
gingival
graft
(FGG)
procedure,
lateral
portions
were
randomly
assigned
receive
(test)
or
no
treatment
(control).
Both
stained
with
hematoxylin-eosin
evaluation
histomorphological
changes
and
Picrosirius
collagen.
Immunohistochemical
staining
performed
antibodies
characterization
inflammatory
infiltrate
neoangiogenesis.
Results:
analysis
failed
show
morphological
variations
both
groups,
demonstrating
that
there
tissue
damage
due
TOT.
prevalence
type
I
collagen
supported
this
finding.
Immune
cells
low
levels
mainly
consisted
CD3+
T
lymphocytes.
histomorphometric
showed
an
increased
average
vessel
area
(7607.95
μm2
±
3983.24
vs.
4038.42
1823.52),
number
caliber
vessels
(49.82
20.55
32.35
16.64),
higher
microvessel
density
(7.89
3.25
vessels/0.26
mm2
5.13
2.63
mm2)
test
group.
Conclusions:
Although
further
investigations
are
needed,
could
improve
speed
up
wound
healing
through
local
condition
hyperoxia.
