Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation DOI Creative Commons
Thanh Tan Vo, Won Beom Jung, Tong Huan Jin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Somatostatin-expressing (SST) interneurons modulate hemodynamic responses both directly and indirectly, but their precise role remains unclear. Here, we investigated how SST affect hemodynamics using a combination of electrophysiology, intrinsic optical imaging, calcium fMRI with pharmacological, optogenetic, chemogenetic manipulations. Prolonged optogenetic stimulation neurons induces fast vasodilation through nitric oxide synthase-expressing that co-express SST, slow mediated by astrocytes. Pharmacological blocking receptors suppresses astrocyte activity delayed vasodilation, while early unchanged. Similar neurovascular coupling mechanisms are observed during prolonged sensory stimulation, which also vasodilation. The the SST-astrocyte pathway, enhances specificity cerebral blood volume-weighted signals to cortical layer 4, as confirmed inhibition neurons. Our findings suggest neuron-astrocyte-vascular pathway shapes is critical for achieving high-specificity, laminar-resolution fMRI, increasingly pursued in human cognitive studies.

Language: Английский

Neocortical somatostatin neuron diversity in cognition and learning DOI Creative Commons
Eunsol Park, Matthew B. Mosso, Alison L. Barth

et al.

Trends in Neurosciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Metabotropic signaling within somatostatin interneurons controls transient thalamocortical inputs during development DOI Creative Commons
Deepanjali Dwivedi, Dimitri Dumontier, Mia Sherer

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 26, 2024

Abstract During brain development, neural circuits undergo major activity-dependent restructuring. Circuit wiring mainly occurs through synaptic strengthening following the Hebbian “fire together, wire together” precept. However, select connections, essential for circuit are transient. They effectively connected early in but strongly diminish during maturation. The mechanisms by which transient connectivity recedes unknown. To investigate this process, we characterize thalamocortical inputs, depress onto somatostatin inhibitory interneurons employing optogenetics, chemogenetics, transcriptomics and CRISPR-based strategies mice. We demonstrate that contrast to typical mechanisms, is non-canonical involves metabotropic signaling. Specifically, metabotropic-mediated transcription, of guidance molecules particular, supports elimination connectivity. Remarkably, found process impacts development normal exploratory behaviors adult

Language: Английский

Citations

6
Dynamic regulation of cortical interneuron wiring DOI Creative Commons

Claudia Rosés-Novella,

C. Bernard

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 102980 - 102980

Published: Feb. 26, 2025

Language: Английский

Citations

0
Developmental dynamics of the prefrontal cortical SST and PV interneuron networks: Insights from the monkey highlight human-specific features DOI
Nafiseh S. Hosseini Fin,

Adrian YS Yip,

Leon Teo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

The primate prefrontal cortex (PFC) is a quintessential hub of cognitive functions. Amidst its intricate neural architecture, the interplay distinct neuronal subtypes, notably parvalbumin (PV) and somatostatin (SST) interneurons (INs), emerge as cornerstone in sculpting cortical circuitry governing processes. While considerable strides have been made elucidating developmental trajectory these neurons rodent models, our understanding their postmigration dynamics primates still needs to be studied. Disruptions this can compromise IN function, impairing signal gating circuit modulation within networks. This study examined expression patterns PV SST, ion transporter KCC2, channel subtypes Kv3.1b, Nav1.1 - associated with morphophysiological stages development postnatal marmoset monkey different frontal regions (granular areas 8aD, 8aV, 9, 46; agranular 11, 47L). Our results demonstrate that maturation PV+ INs extends into adolescence, characterized by discrete epochs specific subtypes. Interestingly, we observed decrease SST interneurons, contrasting studies rodents. endeavor broadens comprehension furnishes invaluable insights etiology pathophysiology neurodevelopmental disorders perturbations function.

Language: Английский

Citations

1
Restoring transient connectivity during development improves dysfunctions in fragile X mice DOI Creative Commons
Dimitri Dumontier, Samuel A Liebman, Viet-Hang Le

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Early-generated circuits are critical for the maturation of cortical network activity and formation excitation/inhibition (E/I) balance. This process involves specific populations inhibitory neurons. While parvalbumin (PV)-expressing neurons have been associated with E/I impairments observed in neurodevelopmental disorders, somatostatin-expressing (SST) recently shown to regulate PV neuron by controlling neural dynamics developing cortex. SST receive transient connections from sensory thalamus, yet implications connectivity disorders remain unknown. Here, we show that thalamocortical is persistent rather than a mouse model Fragile X syndrome. We were able restore using chemogenetics, which led recovery fragile X-associated dysfunctions circuit sensory-dependent behavior. Overall, our findings unveil role early downstream functions.

Language: Английский

Citations

0
Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation DOI Creative Commons
Thanh Tan Vo, Won Beom Jung, Tong Huan Jin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Somatostatin-expressing (SST) interneurons modulate hemodynamic responses both directly and indirectly, but their precise role remains unclear. Here, we investigated how SST affect hemodynamics using a combination of electrophysiology, intrinsic optical imaging, calcium fMRI with pharmacological, optogenetic, chemogenetic manipulations. Prolonged optogenetic stimulation neurons induces fast vasodilation through nitric oxide synthase-expressing that co-express SST, slow mediated by astrocytes. Pharmacological blocking receptors suppresses astrocyte activity delayed vasodilation, while early unchanged. Similar neurovascular coupling mechanisms are observed during prolonged sensory stimulation, which also vasodilation. The the SST-astrocyte pathway, enhances specificity cerebral blood volume-weighted signals to cortical layer 4, as confirmed inhibition neurons. Our findings suggest neuron-astrocyte-vascular pathway shapes is critical for achieving high-specificity, laminar-resolution fMRI, increasingly pursued in human cognitive studies.

Language: Английский

Citations

0