Research Progress on the Mechanism and Application of the Type I CRISPR-Cas System DOI Open Access

Peihong Yang,

Shuai Zhang,

Debao Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12544 - 12544

Published: Nov. 22, 2024

The CRISPR-Cas system functions as an adaptive immune mechanism in archaea and bacteria, providing defense against the invasion of foreign nucleic acids. Most systems are classified into class 1 or 2, with further subdivision several subtypes. primary distinction between 2 lies assembly their effector modules. In systems, complex consists multiple proteins distinct functions, whereas is associated a single protein. Class account for approximately 90% repertoire categorized three types (type I, type IV, III) 12 To date, various have been widely employed field genetic engineering essential tools techniques genome editing. Type I remain valuable resource developing sophisticated application tools. This review provides comprehensive characteristics, mechanisms action, applications well transposon-associated offering effective approaches insights future research on subsequent development systems.

Language: Английский

Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications DOI Creative Commons

Yongjiu Zhang,

Shuxiao Yang,

Zhaofeng Li

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 27, 2025

Cyanobacteria, renowned for their photosynthetic capabilities, serve as efficient microbial chassis capable of converting carbon dioxide into a spectrum bio-chemicals. However, conventional genetic manipulation strategies have proven incompatible with the precise and systematic modifications required in field cyanobacterial synthetic biology. Here, we present an in-depth analysis endogenous CRISPR-Cas systems within genomes, particular focus on Type I systems, which are most widely distributed. We provide comprehensive summary reported DNA defense mechanisms mediated by current applications genome editing. Furthermore, offer insights future these context editing, underscoring potential to revolutionize biology approaches.

Language: Английский

Citations

0
Efforts to Downsize Base Editors for Clinical Applications DOI Open Access
Beomjong Song

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2357 - 2357

Published: March 6, 2025

Since the advent of clustered regularly interspaced short palindromic repeats (CRISPR) system in gene editing field, diverse CRISPR-based tools have been developed for treating genetic diseases. Of these, base editors (BEs) are promising because they can carry out precise at single-nucleotide resolution without inducing DNA double-strand breaks (DSBs), which pose significant risks genomic instability. Despite their outstanding advantages, clinical application BEs remains challenging due to large size, limits efficient delivery, particularly adeno-associated virus (AAV)-based systems. To address this issue, various strategies explored reduce size BEs. These approaches include truncating nonessential domains and replacing bulky components with smaller substitutes compromising efficiency. In review, we highlight importance downsizing therapeutic applications introduce recent advances size-reduction strategies. Additionally, ongoing efforts overcome other limitations BEs, providing insights into potential improving vivo editing.

Language: Английский

Citations

0
Advancements in CRISPR-Cas-Based Strategies for Combating Antimicrobial Resistance DOI
Ruiting Zhang, Qi Zhou,

Siying Huang

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128232 - 128232

Published: May 1, 2025

Language: Английский

Citations

0
Research Progress and Application of Miniature CRISPR-Cas12 System in Gene Editing DOI Open Access

Qiangbing Xuan,

Junjie Wang, Yingbin Nie

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12686 - 12686

Published: Nov. 26, 2024

CRISPR-Cas system, a natural acquired immune system in prokaryotes that defends against exogenous DNA invasion because of its simple structure and easy operation, has been widely used many research fields such as synthetic biology, crop genetics breeding, precision medicine, so on. The miniature CRISPR-Cas12 an emerging genome editing tool recent years. Compared to the commonly CRISPR-Cas9 CRISPR-Cas12a, unique advantages, rich PAM sites, higher specificity, smaller volume, cytotoxicity. However, application Cas12 proteins methods improve efficiency have not systematically summarized. In this review, we introduce classification summarize structural characteristics type V cleavage mechanism five proteins. gene animals, plants, microorganisms is summarized, strategies are discussed, aiming provide reference for further understanding functional engineering modification system.

Language: Английский

Citations

3
Efficient, compact, and versatile: Type I‐F2 CRISPR‐Cas system DOI Creative Commons
Shengsheng Ma, Senfeng Zhang,

K. Liu

et al.

mLife, Journal Year: 2024, Volume and Issue: 3(3), P. 384 - 386

Published: Sept. 1, 2024

Language: Английский

Citations

0
Research Progress on the Mechanism and Application of the Type I CRISPR-Cas System DOI Open Access

Peihong Yang,

Shuai Zhang,

Debao Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12544 - 12544

Published: Nov. 22, 2024

The CRISPR-Cas system functions as an adaptive immune mechanism in archaea and bacteria, providing defense against the invasion of foreign nucleic acids. Most systems are classified into class 1 or 2, with further subdivision several subtypes. primary distinction between 2 lies assembly their effector modules. In systems, complex consists multiple proteins distinct functions, whereas is associated a single protein. Class account for approximately 90% repertoire categorized three types (type I, type IV, III) 12 To date, various have been widely employed field genetic engineering essential tools techniques genome editing. Type I remain valuable resource developing sophisticated application tools. This review provides comprehensive characteristics, mechanisms action, applications well transposon-associated offering effective approaches insights future research on subsequent development systems.

Language: Английский

Citations

0