Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis DOI Open Access
Lucie Crhák Khaitová, Pavlina Mikulková, Jana Pecinkova

et al.

Published: Sept. 20, 2023

Heat stress is a major threat to global crop production, and understanding its impact on plant fertility crucial for developing climate-resilient crops. Despite the known negative effects of heat reproduction, underlying molecular mechanisms remain poorly understood. Here, we investigated elevated temperature centromere structure chromosome segregation during meiosis in Arabidopsis thaliana . Our findings reveal that causes significant decline leads formation micronuclei pollen mother cells, along with an extended duration meiotic division. We also demonstrate reduction amounts centromeric histone kinetochore protein BMF1 at centromeres increasing temperature. Furthermore, show prolongs activity spindle assembly checkpoint I, indicating impaired efficiency attachments microtubules. analysis mutants reduced suggests weakened sensitize plants temperature, resulting defects even moderate temperatures. These results indicate functionality are highly sensitive stress, suggest kinetochores may represent bottleneck adaptation

Language: Английский

Regulation of Coffea arabica floral development, flowering and fruit maturation by plant growth regulators DOI
Raphael Ricon de Oliveira, Muhammad Noman, Lillian Magalhães Azevedo

et al.

Advances in botanical research, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

2
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis DOI Creative Commons
Lucie Crhák Khaitová, Pavlina Mikulková, Jana Pecinkova

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Sept. 20, 2023

Heat stress is a major threat to global crop production, and understanding its impact on plant fertility crucial for developing climate-resilient crops. Despite the known negative effects of heat reproduction, underlying molecular mechanisms remain poorly understood. Here, we investigated elevated temperature centromere structure chromosome segregation during meiosis in Arabidopsis thaliana. Consistent with previous studies, leads decline micronuclei formation pollen mother cells. Our results reveal that causes decrease amount centromeric histone kinetochore protein BMF1 at meiotic centromeres increasing temperature. Furthermore, show increases duration divisions prolongs activity spindle assembly checkpoint I, indicating an impaired efficiency attachments microtubules. analysis mutants reduced levels suggests weakened sensitize plants temperature, resulting defects even moderate temperatures. These indicate functionality are highly sensitive stress, suggest kinetochores may represent critical bottleneck adaptation

Language: Английский

Citations

6
Haploid induction: an overview of parental factor manipulation during seed formation DOI Creative Commons
Jingpu Song, Raju Datla, Jitao Zou

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 4, 2024

In plants,

Language: Английский

Citations

1
Coffee microsporogenesis and related small interfering RNAs biosynthesis have a unique pattern among eudicots suggesting a sensitivity to climate changes DOI Creative Commons
Kellen Kauanne Pimenta de Oliveira, Raphael Ricon de Oliveira, Gabriel de Campos Rume

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 7, 2023

ABSTRACT Recently, the siRNAs pathways, and especially reproductive phasiRNAs, have attracted attention in eudicots since their biological roles are still unknown biogenesis took different evolutionary pathways compared to monocots. In this work, we used Coffea arabica L., a recently allotetraploid formed from hybridization of C. canephora eugenioides unreduced gametes, explore microsporogenesis small RNAs related eudicot crop. First, identified stages during anther development revealing that pre-meiosis occurs anthers 1.5 mm inside floral buds (FBs), whereas meiosis between 4.2 FBs, post-meiosis FBs larger than 4.2mm. These coincide with Brazilian winter, period reduced growth which suggests temperature sensitivity. Next, quantified expression 21- 24-nt phasiRNAs coffee together canonical novel miRNA triggers, characterized DCL AGO families. Our results showed pattern phasiRNA abundance is unique among described trigger car-miR2275 involved processing both 21 24 nt phasiRNAs. Fourteen genes were identified, but DCL5, biosynthesis monocots, was not according its specificity for Thus, our work explored knowledge gap about coffee, contributing control improvement fertility eudicots.

Language: Английский

Citations

3
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis DOI Creative Commons
Lucie Crhák Khaitová, Pavlina Mikulková, Jana Pecinkova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 21, 2023

Abstract Heat stress is a major threat to global crop production, and understanding its impact on plant fertility crucial for developing climate-resilient crops. Despite the known negative effects of heat reproduction, underlying molecular mechanisms remain poorly understood. Here, we investigated elevated temperature centromere structure chromosome segregation during meiosis in Arabidopsis thaliana . Consistent with previous studies, leads decline micronuclei formation pollen mother cells. Our results reveal that causes decrease amount centromeric histone kinetochore protein BMF1 at meiotic centromeres increasing temperature. Furthermore, show increases duration divisions prolongs activity spindle assembly checkpoint I, indicating an impaired efficiency attachments microtubules. analysis mutants reduced levels suggests weakened sensitize plants temperature, resulting defects even moderate temperatures. These indicate functionality are highly sensitive stress, suggest kinetochores may represent critical bottleneck adaptation

Language: Английский

Citations

2
Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants DOI Open Access

Huiqi Fu,

Jiaqi Zhong,

Jiayi Zhao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 14, 2024

Abstract Ultraviolet (UV) radiation influences development and genome stability in organisms; however, its impacts on meiosis, a special cell division essential for the delivery of genetic information over generations eukaryotes, remain not yet elucidated. In this study, we demonstrate that UV attenuates centromere-mediated meiotic chromosome induces unreduced gametes Arabidopsis thaliana . We show reduces crossover (CO) rate but does interfere with integrity. Functional centromere-specific histone 3 (CENH ) is required obligate CO formation, plays role protection homolog synapsis sister-chromatid cohesion under stress. Moreover, specifically alters orientation organization spindles phragmoplasts at meiosis II, resulting restitution gametes. Further, determine UV-induced rely Resistance Locus8-mediated perception Tapetal Development Function1- Aborted Microspores-dependent tapetum development, occurs possibly via impacted JASON function downregulated Parallel Spindle1. This study sheds light gametophytic ploidy consistency, which thus may influence evolution flowering plants.

Language: Английский

Citations

0
Ultraviolet attenuates centromere‐mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants DOI

Huiqi Fu,

Jiaqi Zhong,

Jiayi Zhao

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(6), P. 2214 - 2234

Published: July 22, 2024

Ultraviolet (UV) radiation influences development and genome stability in organisms; however, its impact on meiosis, a special cell division essential for the delivery of genetic information across generations eukaryotes, has not yet been elucidated. In this study, by performing cytogenetic studies, we reported that UV does damage meiotic chromosome integrity but attenuates centromere-mediated induces unreduced gametes Arabidopsis thaliana. We showed functional centromere-specific histone 3 (CENH3) is required obligate crossover formation plays role protection sister chromatid cohesion under stress. Moreover, found specifically alters orientation organization spindles phragmoplasts at meiosis II, resulting restitution gametes. determined UV-induced rely Resistance Locus8-mediated perception Tapetal Development Function1- Aborted Microspores-dependent tapetum development, possibly occurs via altered JASON function downregulated Parallel Spindle1. This study provides evidence gametophytic ploidy consistency flowering plants.

Language: Английский

Citations

0
Report of a Spontaneous Potato Monoploid Resulting from a Biparental Diploid Potato Cross DOI

N. D. Williams,

Greg Kornelis,

Ek Han Tan

et al.

American Journal of Potato Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Language: Английский

Citations

0
CRISPR/Cas9 and Anther Culture for Precision Double Haploid Line Production in Controlled Environments DOI Open Access
Avinash Sharma, Himanshu Pandey, Varucha Misra

et al.

Plant Breeding, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

ABSTRACT The development of mapping populations and quantitative trait loci (QTL) analysis face constraints, in crops exhibiting male sterility self‐incompatibility under field conditions. Addressing these challenges requires the integration advanced techniques, including temporal alteration or excision centromere histone H3 (CENH3) protein use gene editing tools such as MATRILINEAL (MTL) knockout. Specifically, this can be achieved through Cas9/gRNA‐mediated mutagenesis Cas9/gRNA‐driven promoter expression systems. These technologies offer efficient means to advance QTL sterile self‐incompatible within controlled ecosystems. doubled haploid (DH) population, traditionally requiring 3 years generation time via anther culture method, now expedited 2–3 using techniques environmental Notably, DH efficiently generated various crops, rice, wheat, maize, barley oats by leveraging tools. Among tools, novel approach CENH3 alteration/excision emerges highly compared MTL knockout mutation Cas9/gRNA expression. However, further investigation is warranted optimise regeneration double enhance

Language: Английский

Citations

0
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis DOI Open Access
Lucie Crhák Khaitová, Pavlina Mikulková, Jana Pecinkova

et al.

Published: Sept. 20, 2023

Heat stress is a major threat to global crop production, and understanding its impact on plant fertility crucial for developing climate-resilient crops. Despite the known negative effects of heat reproduction, underlying molecular mechanisms remain poorly understood. Here, we investigated elevated temperature centromere structure chromosome segregation during meiosis in Arabidopsis thaliana . Our findings reveal that causes significant decline leads formation micronuclei pollen mother cells, along with an extended duration meiotic division. We also demonstrate reduction amounts centromeric histone kinetochore protein BMF1 at centromeres increasing temperature. Furthermore, show prolongs activity spindle assembly checkpoint I, indicating impaired efficiency attachments microtubules. analysis mutants reduced suggests weakened sensitize plants temperature, resulting defects even moderate temperatures. These results indicate functionality are highly sensitive stress, suggest kinetochores may represent bottleneck adaptation

Language: Английский

Citations

0