Cold Spring Harbor Protocols,
Journal Year:
2023,
Volume and Issue:
2023(11), P. pdb.prot108096 - pdb.prot108096
Published: March 20, 2023
Loss
of
vision
is
a
prominent
feature
aging
and
considered
by
many
to
be
the
most
valuable
sense
lost.
In
our
graying
society,
we
are
increasingly
challenged
age-related
deterioration
central
nervous
system
(CNS),
as
well
age-associated
neurodegenerative
diseases
brain
injuries,
all
often
affecting
visual
thus
its
performance.
Here,
describe
two
visually
driven
behavior
assays
evaluate
performance
upon
or
CNS
damage
in
fast-aging
killifish.
The
first
test,
optokinetic
response
(OKR),
measures
reflexive
eye
movement
triggered
motion
field
allows
assessment
acuity.
second
assay,
dorsal
light
reflex
(DLR),
evaluates
swimming
angle
based
on
input
coming
from
above.
OKR
can
used
study
effect
acuity
improvement
recovery
after
rejuvenation
therapy
injury
disease,
whereas
DLR
best
assess
functional
repair
unilateral
optic
nerve
crush.
Cell,
Journal Year:
2024,
Volume and Issue:
187(16), P. 4150 - 4175
Published: Aug. 1, 2024
Cellular
senescence
is
a
cell
fate
triggered
in
response
to
stress
and
characterized
by
stable
cell-cycle
arrest
hypersecretory
state.
It
has
diverse
biological
roles,
ranging
from
tissue
repair
chronic
disease.
The
development
of
new
tools
study
vivo
paved
the
way
for
uncovering
its
physiological
pathological
roles
testing
senescent
cells
as
therapeutic
target.
However,
lack
specific
broadly
applicable
markers
makes
it
difficult
identify
characterize
tissues
living
organisms.
To
address
this,
we
provide
practical
guidelines
called
"minimum
information
cellular
experimentation
vivo"
(MICSE).
presents
an
overview
rodent
tissues,
transgenic
models,
non-mammalian
systems,
human
tumors
their
use
identification
specification
cells.
These
uniform,
state-of-the-art,
accessible
toolset
improve
our
understanding
vivo.
The
African
turquoise
killifish
is
a
powerful
vertebrate
system
to
study
complex
phenotypes
at
scale,
including
aging
and
age-related
disease.
Here,
we
develop
rapid
precise
CRISPR/Cas9-mediated
knock-in
approach
in
the
killifish.
We
show
its
efficient
application
precisely
insert
fluorescent
reporters
of
different
sizes
various
genomic
loci
order
drive
cell-type-
tissue-specific
expression.
This
method
should
allow
establishment
humanized
disease
models
development
cell-type-specific
molecular
probes
for
studying
biology.
Frontiers in Aging Neuroscience,
Journal Year:
2024,
Volume and Issue:
16
Published: Feb. 28, 2024
Sensorineural
hearing
loss
(SNHL)
is
a
category
of
that
often
leads
to
difficulty
in
understanding
speech
and
other
sounds.
Auditory
system
dysfunction,
including
deafness
auditory
trauma,
results
cognitive
deficits
via
neuroplasticity.
Cognitive
impairment
(CI)
refers
an
abnormality
the
brain’s
higher
intellectual
processes
related
learning,
memory,
thinking
judgment
can
lead
severe
learning
memory
deficits.
Studies
have
established
strong
correlation
between
SNHL
CI,
but
it
remains
unclear
how
contributes
CI.
The
purpose
this
article
describe
three
hypotheses
regarding
relationship,
mainstream
load
hypothesis,
co-morbidity
sensory
deprivation
as
well
latest
research
progress
each
hypothesis.
Ageing Research Reviews,
Journal Year:
2023,
Volume and Issue:
90, P. 102019 - 102019
Published: July 22, 2023
Turquoise
killifish
(Nothobranchius
furzeri)
are
naturally
short-lived
vertebrates
that
display
a
wide
range
of
spontaneous
age-related
changes,
including
onset
cancer,
reduced
mobility,
and
cognitive
decline.
Here,
we
focus
on
describing
the
phenotypic
spectrum
aging
brain.
As
turquoise
age,
their
dopaminergic
noradrenergic
neurons
undergo
significant
decline
in
number.
Furthermore,
brain
is
associated
with
several
glial-specific
such
as
an
increase
astrocyte-covered
surface
area
numbers
microglial
cells,
i.e.
brain-specific
macrophage
population.
Killifish
brains
age-dependent
proteasome
activity
increased
protein
aggregation,
aggregation
Parkinson's
disease
marker
α-synuclein.
Parallel
to
degeneration,
develop
gut
dysbiosis,
which
has
been
proposed
affect
human
neurodegenerative
disease.
Finally,
aged
declined
learning
capacity.
We
argue
that,
taken
together,
molecular,
cellular
functional
changes
spontaneously
take
place
during
consistent
robust
degenerative
process
shares
remarkable
similarities
diseases.
Hence,
propose
represent
powerful
model
degeneration
can
be
effectively
used
explore
causal
mechanisms
underlying
npj Regenerative Medicine,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: June 16, 2023
The
young
African
turquoise
killifish
has
a
high
regenerative
capacity,
but
loses
it
with
advancing
age,
adopting
several
aspects
of
the
limited
form
mammalian
regeneration.
We
deployed
proteomic
strategy
to
identify
pathways
that
underpin
loss
power
caused
by
aging.
Cellular
senescence
stood
out
as
potential
brake
on
successful
neurorepair.
applied
senolytic
cocktail
Dasatinib
and
Quercetin
(D
+
Q)
test
clearance
chronic
senescent
cells
from
aged
central
nervous
system
(CNS)
well
rebooting
neurogenic
output.
Our
results
show
entire
telencephalon
holds
very
cell
burden,
including
parenchyma
niches,
which
could
be
diminished
short-term,
late-onset
D
Q
treatment.
Reactive
proliferation
non-glial
progenitors
increased
substantially
lead
restorative
neurogenesis
after
traumatic
brain
injury.
provide
cellular
mechanism
for
age-related
regeneration
resilience
proof-of-concept
therapy
revive
in
an
already
or
diseased
CNS.
Aging Cell,
Journal Year:
2024,
Volume and Issue:
23(8)
Published: May 14, 2024
Abstract
Age‐related
vision
loss
caused
by
retinal
neurodegenerative
pathologies
is
becoming
more
prevalent
in
our
ageing
society.
To
understand
the
physiological
and
molecular
impact
of
on
homeostasis,
we
used
short‐lived
African
turquoise
killifish,
a
model
known
to
naturally
develop
central
nervous
system
(CNS)
hallmarks
loss.
Bulk
single‐cell
RNA‐sequencing
(scRNAseq)
three
age
groups
(6‐,
12‐,
18‐week‐old)
identified
transcriptional
fingerprints
killifish
retina,
unveiling
pathways
also
aged
brain,
including
oxidative
stress,
gliosis,
inflammageing.
These
findings
were
comparable
observations
mouse
retina.
Additionally,
changes
genes
related
diseases,
such
as
glaucoma
age‐related
macular
degeneration,
observed.
The
cellular
heterogeneity
retina
was
characterized,
confirming
presence
all
typical
vertebrate
cell
types.
Data
integration
from
age‐matched
samples
between
bulk
scRNAseq
experiments
revealed
specificity
gene
expression
upon
ageing,
suggesting
potential
disruption
homeostasis.
Differential
analysis
within
types
highlighted
role
glial/immune
cells
important
stress
regulators
during
ageing.
Our
work
emphasizes
value
fast‐ageing
elucidating
signatures
age‐associated
disease
decline.
This
study
contributes
understanding
how
may
CNS
health,
providing
insights
that
inform
future
therapeutic
strategies
for
pathologies.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Abstract
Aging
is
associated
with
progressive
tissue
dysfunction,
leading
to
frailty
and
mortality.
Characterizing
aging
features,
such
as
changes
in
gene
expression
dynamics,
shared
across
tissues
or
specific
each
tissue,
crucial
for
understanding
systemic
local
factors
contributing
the
process.
We
performed
RNA-sequencing
on
13
at
6
different
ages
African
turquoise
killifish,
shortest-lived
vertebrate
that
can
be
raised
captivity.
This
comprehensive,
sex-balanced
‘atlas’
dataset
reveals
varying
strength
of
sex-age
interactions
killifish
identifies
age-altered
biological
pathways
are
evolutionarily
conserved.
Demonstrating
utility
this
resource,
we
discovered
head
kidney
exhibits
a
myeloid
bias
during
aging,
phenomenon
more
pronounced
females
than
males.
In
addition,
developed
tissue-specific
‘transcriptomic
clocks’
identified
biomarkers
predictive
chronological
age.
show
importance
sex-specific
clocks
selected
use
evaluate
dietary
intervention
killifish.
Our
work
provides
comprehensive
resource
studying
dynamics
powerful
model.
