Single‐cell sequencing unveils the impact of aging on the progenitor cell diversity in the telencephalon of the female killifish N. furzeri

Aging Cell, Journal Year: 2024, Volume and Issue: 23(10)

Published: July 1, 2024

Abstract The African turquoise killifish ( Nothobranchius furzeri ) combines a short lifespan with spontaneous age‐associated loss of neuro‐regenerative capacity, an intriguing trait atypical for teleost. impact aging on the cellular composition adult stem cell niches, leading to this dramatic decline in postnatal neuro‐ and gliogenesis, remains elusive. Single‐cell RNA sequencing telencephalon young female short‐lived GRZ‐AD strain unveiled progenitors glial non‐glial nature, different excitatory inhibitory neuron subtypes, as well non‐neural types. Sub‐clustering identified four radial glia (RG) types, two progenitor (NGP) intermediate (intercell) states. Two astroglia‐like, one ependymal, neuroepithelial‐like (NE) RG subtype were found at locations forebrain line their role, while proliferative, active NGPs spread throughout. Lineage inference pointed NE‐RG start intercessor populations glio‐ neurogenesis. Upon aging, single‐cell revealed major perturbations proportions astroglia intercell states, molecular signatures specific including altered MAPK, mTOR, Notch, Wnt pathways. This catalog regeneration‐competent telencephalon, combined evidence aging‐related transcriptomic changes, presents useful resource understand basis age‐dependent neuroplasticity. data is also available through online database killifishbrain_scseq ).

Language: Английский

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Circadian Modulation of Behavioral Stress Responses in Zebrafish Is Age‐Dependent

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

ABSTRACT In the wild, stressors occur with varying likelihood throughout day, leading animals to evolve plastic stress responses that exhibit circadian rhythmicity. mammals, studies have revealed plasticity of response may differ age. However, such developmental effects been largely overlooked in other vertebrate groups. our research, we explored presence variation daily pattern behavioral a teleost fish model: zebrafish ( Danio rerio ). We compared juvenile and adult individuals two paradigms commonly used analyze response, as open‐field test diving test. Our comparisons were conducted every 4 h during 24‐h cycle variations. Significant rhythms detected for almost all analyzed behaviors both tests. general, analyses suggested greater adults daytime juveniles night‐time, although not indicators aligned this direction. Moreover, found average differences behavior, suggesting more sensitive stress. Overall, these findings highlight importance considering non‐mammalian species like zebrafish.

Language: Английский

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Age-related dysregulation of the retinal transcriptome in African turquoise killifish

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 23, 2024

Age-related vision loss caused by retinal neurodegenerative pathologies is becoming more prevalent in our ageing society. To understand the physiological and molecular impact of on homeostasis, we used short-lived African turquoise killifish, a model known to naturally develop central nervous system (CNS) hallmarks loss. Bulk single-cell RNA-sequencing (scRNA-seq) three age groups (6-, 12-, 18-week-old) identified transcriptional fingerprints killifish retina, unveiling pathways also aged brain, including oxidative stress, gliosis, inflammageing. These findings were comparable observations mouse retina. Additionally, changes genes related diseases, such as glaucoma age-related macular degeneration, observed. The cellular heterogeneity retina was characterised, confirming presence all typical vertebrate cell types. Data integration from age-matched samples between bulk scRNA-seq experiments revealed specificity gene expression upon ageing, suggesting potential disruption homeostasis. Differential analysis within types highlighted role glial/immune cells important stress regulators during ageing. Our work emphasises value fast-ageing elucidating signatures age-associated disease decline. This study contributes understanding how may CNS health, providing insights that inform future therapeutic strategies for pathologies.

Language: Английский

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Olfactory and gustatory chemical sensor systems in the African turquoise killifish: Insights from morphology

Cell and Tissue Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract Smell and taste are extensively studied in fish species as essential for finding food selecting mates while avoiding toxic substances predators. Depending on the evolutionary position adaptation, a discrete variation morphology of these sense organs has been reported numerous teleost species. Here, first time, we approach phenotypic characterization olfactory epithelium buds African turquoise killifish ( Nothobranchius furzeri ), model organism known its short lifespan use ageing research. Our observations indicate that N. is organized simple patch, lacking complex folding into rosette, with an average size approximately 600 µm length, 300 width, 70 thickness. Three main cytotypes, including receptor neurons (CalbindinD28K), supporting cells (β-tubulin IV), basal (Ki67), were identified across epithelium. Further, determined buds’ distribution quantification between anterior (skin, lips, oral cavity) posterior (gills, pharynx, oesophagus) systems. We key cytotypes by using immunohistochemical markers, i.e. CalbindinD28K, doublecortin, neuropeptide Y (NPY) gustatory cells, glial fibrillary acidic protein (GFAP) Ki67, marker cellular proliferation cells. Altogether, results microsmatic unique features possesses well-developed system compared to anterior. This study provides fundamental insights chemosensory biology , facilitating future investigations nutrient-sensing mechanisms their roles development, survival, ageing.

Language: Английский

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