Visually Driven Behavior Assays to Study Functionality and Recovery after Damage of the Aged African Turquoise Killifish Visual System DOI
Steven Bergmans, Sophie Vanhunsel, Lieve Moons

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2023(11), P. pdb.prot108096 - pdb.prot108096

Published: March 20, 2023

Loss of vision is a prominent feature aging and considered by many to be the most valuable sense lost. In our graying society, we are increasingly challenged age-related deterioration central nervous system (CNS), as well age-associated neurodegenerative diseases brain injuries, all often affecting visual thus its performance. Here, describe two visually driven behavior assays evaluate performance upon or CNS damage in fast-aging killifish. The first test, optokinetic response (OKR), measures reflexive eye movement triggered motion field allows assessment acuity. second assay, dorsal light reflex (DLR), evaluates swimming angle based on input coming from above. OKR can used study effect acuity improvement recovery after rejuvenation therapy injury disease, whereas DLR best assess functional repair unilateral optic nerve crush.

Language: Английский

Early-life growth and cellular heterogeneity in the short-lived African turquoise killifish telencephalon DOI Creative Commons
Caroline Zandecki, Valerie Mariën, Rajagopal Ayana

et al.

Biology Open, Journal Year: 2025, Volume and Issue: 14(4)

Published: April 15, 2025

ABSTRACT The African turquoise killifish (Nothobranchius furzeri) is becoming a favorable model for neurobiological research. combination of short lifespan and declining neuroregenerative capacity upon aging makes it ideally suited research on brain regeneration. A remarkable cellular diversity up the young-adult telencephalon, characterized by highly proliferative non-glial progenitors spatially distinct radial glia subtypes. In contrast to relatively slow embryonic development, hatching followed period accelerated growth, in which experiences rapid expansion maturation. this study, we quantified growth progression maturation telencephalon during early post-embryonic development. We discovered that, similar zebrafish, neuro-epithelial cells abut neurogenic niches from life onwards. Spatial data revealed qualitative quantitative differences along anterior-posterior axis between pallial subpallial regions terms pace. confirmed generation GABAergic neurons niche glutamatergic two niches. Our further showed more widespread appearance inhibitory at compared zebrafish.

Language: Английский

Citations

0
The age factor in optic nerve regeneration: Intrinsic and extrinsic barriers hinder successful recovery in the short‐living killifish DOI Creative Commons
Sophie Vanhunsel, Steven Bergmans, An Beckers

et al.

Aging Cell, Journal Year: 2021, Volume and Issue: 21(1)

Published: Dec. 19, 2021

As the mammalian central nervous system matures, its regenerative ability decreases, leading to incomplete or non-recovery from neurodegenerative diseases and insults that we are increasingly facing in our aging world population. Current neuroregenerative research is largely directed toward identifying molecular cellular players underlie repair, yet it repeatedly ignores context which many of these appear. Using an optic nerve crush model a novel biogerontology model, is, short-living African turquoise killifish, impact on injury-induced repair was investigated. This work reveals age-related decline axonal regeneration female with different phases process being affected depending age. Interestingly, as mammals, both reduced intrinsic growth potential non-supportive environment seem lie at basis this impairment. Overall, introduce killifish visual age-dependent identify new targets for neurorepair non-regenerating individuals, thereby also considering effects neurorepair.

Language: Английский

Citations

19
A review on neurodegeneration in the fast-ageing killifish, the first animal model to study the natural occurrence of neuronal cell loss DOI
Steven Bergmans, Laura Raes, Lieve Moons

et al.

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 91, P. 102065 - 102065

Published: Sept. 3, 2023

Language: Английский

Citations

7
Fish microglia: Beyond the resident macrophages of the central nervous system - A review of their morphofunctional characteristics. DOI Creative Commons
José Carlos Campos‐Sánchez,

José Meseguer,

Francisco A. Guardiola

et al.

Developmental & Comparative Immunology, Journal Year: 2024, Volume and Issue: unknown, P. 105274 - 105274

Published: Sept. 1, 2024

Language: Английский

Citations

2
Amyloid Beta Precursor Protein contributes to brain aging and learning decline in short-lived turquoise killifish (Nothobranchius furzeri) DOI Creative Commons
Dennis E. M. de Bakker,

Mihaela Mihaljević,

Kunal Gharat

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract Amyloid beta (Aβ) accumulation is associated with inflammation, neurodegeneration, and cognitive decline in the context of neurodegenerative diseases. However, its role during normal aging remains largely unexplored. In this study, we investigated natural impact Aβ precursor protein ( app ) on brain using a short-lived vertebrate model, turquoise killifish Nothobranchius furzeri ). We identified amyloid derivatives across different age groups found that pyroglutamated —a neurotoxic variant—accumulates intra-neuronally an age-dependent manner, co-localizing apoptosis marker TUNEL. To determine whether contributes to spontaneous aging, used CRISPR/Cas9 generate “amyloid a” appa knock-out strain. Notably, −/− mutants exhibited reduced cell death overall younger proteome, as well improved learning capacity old age. Taken together, broadly affects making it promising target for anti-aging interventions.

Language: Английский

Citations

2
Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types DOI Creative Commons

Aanandita Kothurkar,

Gregory S. Patient, Nicole C. L. Noel

et al.

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(23)

Published: Nov. 14, 2024

ABSTRACT To understand the multicellular composition of tissues, and how it is altered during development, ageing and/or disease, we must visualise complete cellular landscape. Currently, this hindered by our limited ability to combine multiple markers. overcome this, adapted a highly multiplexed immunofluorescence (IF) technique called ‘Iterative Bleaching Extends Multiplexity’ (IBEX) zebrafish retina. We optimised fluorescent antibody micro-conjugation perform sequential rounds labelling on single tissue simultaneously all major retinal cell types with 11 cell-specific antibodies. further IBEX be compatible transgenic reporter lines, in situ hybridisation chain reaction (HCR), whole-mount (WMIF). applied at stages study spatial temporal relationships between glia neurons development. Finally, demonstrate utility across species testing turquoise killifish (Nothobranchius furzeri) African clawed frog (Xenopus laevis) glean large amounts information from precious tissues. These techniques will revolutionise any organism where antibodies are readily available.

Language: Английский

Citations

2
Molecular and Biochemical Analyses of Aging Hallmarks in the Central Nervous System of the Fast-Aging African Turquoise Killifish DOI
Steven Bergmans, Sophie Vanhunsel, Caroline Zandecki

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2023(10), P. pdb.prot107810 - pdb.prot107810

Published: March 15, 2023

As modern society is graying, aging research and biogerontology models, in which the process can be studied, are becoming increasingly important. A proper model defined as one that displays many of hallmarks. Here, we provide two different practical approaches—namely, real-time quantitative polymerase chain reaction (RT-qPCR) western blotting—that used to investigate cellular senescence (RT-qPCR for p21 p27 ), altered intercellular communication/inflammaging il-10 , sirt-1 il-6 il-1b il-8 tnf oxidative stress (western blotting 4-HNE) killifish central nervous system, and, more specifically, retina, optic tectum, telencephalon. These molecular biochemical analyses a first step confirming characteristics but should preferably combined with morphological analyses.

Language: Английский

Citations

5
Morphological Analysis of Aging Hallmarks in the Central Nervous System of the Fast-Aging African Turquoise Killifish DOI
Steven Bergmans, Sophie Vanhunsel, Jolien Van houcke

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2023(10), P. pdb.prot107827 - pdb.prot107827

Published: March 20, 2023

As the number of elderly individuals is increasing in modern society, need for a relevant gerontology model higher than ever before. Aging can be defined by specific cellular hallmarks, described López-Otín and colleagues, who provided map which used to scavenge aging tissue environment. revealing presence individual hallmarks does not necessarily indicate aging, here we provide different (immuno)histochemical approaches that investigate several hallmarks—namely, genomic damage, mitochondrial dysfunction/oxidative stress, senescence, stem cell exhaustion, altered intercellular communication—in killifish retina, optic tectum, and/or telencephalon at morphological level. In combination with molecular biochemical analysis these this protocol offers opportunity fully characterize aged central nervous system.

Language: Английский

Citations

4
Neurotrophins and Trk Neurotrophin Receptors in the Retina of Adult Killifish (Nothobranchius guentheri) DOI Open Access
Caterina Porcino, Kamel Mhalhel, Marilena Briglia

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2732 - 2732

Published: Feb. 27, 2024

Specific subpopulations of neurons in nerve and sensory systems must be developed maintained, this is accomplished significant part by neurotrophins (NTs) the signaling receptors on which they act, called tyrosine protein kinase (Trks). The neurotrophins–tyrosine (NTs/Trks) system involved organ regulation, including visual system. An NTs/Trks alteration associated with neurodegeneration related to aging diseases, retinal pathologies. emergent model field translational medicine, for instance, study, annual killifish belonging Nothobranchius genus, thanks its short lifespan. Members such as guentheri, humans share a similar stratigraphy. Nevertheless, according authors’ knowledge, occurrence distribution retina N. guentheri has never been investigated before. Therefore, present study aimed localize neurotrophin BDNF, NGF, NT-3 TrkA, TrkB, TrkC using immunofluorescence method. investigation demonstrates, first time, and, consequently, potential key role these proteins biology survival cells.

Language: Английский

Citations

1
Tissue stretching is a confounding factor for the evaluation of neurodegeneration in the fast-ageing killifish DOI
Steven Bergmans, Pieter‐Jan Serneels, Luca Masin

et al.

Biogerontology, Journal Year: 2023, Volume and Issue: 24(3), P. 403 - 419

Published: March 13, 2023

Language: Английский

Citations

3