Visually Driven Behavior Assays to Study Functionality and Recovery after Damage of the Aged African Turquoise Killifish Visual System DOI
Steven Bergmans, Sophie Vanhunsel, Lieve Moons

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2023(11), P. pdb.prot108096 - pdb.prot108096

Published: March 20, 2023

Loss of vision is a prominent feature aging and considered by many to be the most valuable sense lost. In our graying society, we are increasingly challenged age-related deterioration central nervous system (CNS), as well age-associated neurodegenerative diseases brain injuries, all often affecting visual thus its performance. Here, describe two visually driven behavior assays evaluate performance upon or CNS damage in fast-aging killifish. The first test, optokinetic response (OKR), measures reflexive eye movement triggered motion field allows assessment acuity. second assay, dorsal light reflex (DLR), evaluates swimming angle based on input coming from above. OKR can used study effect acuity improvement recovery after rejuvenation therapy injury disease, whereas DLR best assess functional repair unilateral optic nerve crush.

Language: Английский

The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes DOI Open Access
Felix Boos, Jingxun Chen, Anne Brunet

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2024(3), P. pdb.over107737 - pdb.over107737

Published: April 26, 2023

The African turquoise killifish

Language: Английский

Citations

3
Rapid and precise genome engineering in a naturally short-lived vertebrate DOI Creative Commons
Ravi D. Nath, Claire N. Bedbrook, Rahul Nagvekar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: May 26, 2022

Abstract The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop rapid precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application precisely insert fluorescent reporters of different sizes various genomic loci, drive cell-type- tissue-specific expression. This method should allow establishment humanized disease models development cell-type-specific molecular probes for studying biology.

Language: Английский

Citations

5
Some Aspects of Development and Histological Structure of the Visual System of Nothobranchius Guentheri DOI Creative Commons
Dmitry Nikiforov-Nikishin, В. А. Ирха, Nikita Kochetkov

et al.

Animals, Journal Year: 2021, Volume and Issue: 11(9), P. 2755 - 2755

Published: Sept. 21, 2021

In this, work some aspects of the development visual system

Language: Английский

Citations

6
Killifish switch towards mammalian-like regeneration upon aging DOI Creative Commons
Sophie Vanhunsel, Steven Bergmans, Lieve Moons

et al.

Aging, Journal Year: 2022, Volume and Issue: 14(7), P. 2924 - 2925

Published: March 31, 2022

Language: Английский

Citations

4
Visually Driven Behavior Assays to Study Functionality and Recovery after Damage of the Aged African Turquoise Killifish Visual System DOI
Steven Bergmans, Sophie Vanhunsel, Lieve Moons

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2023(11), P. pdb.prot108096 - pdb.prot108096

Published: March 20, 2023

Loss of vision is a prominent feature aging and considered by many to be the most valuable sense lost. In our graying society, we are increasingly challenged age-related deterioration central nervous system (CNS), as well age-associated neurodegenerative diseases brain injuries, all often affecting visual thus its performance. Here, describe two visually driven behavior assays evaluate performance upon or CNS damage in fast-aging killifish. The first test, optokinetic response (OKR), measures reflexive eye movement triggered motion field allows assessment acuity. second assay, dorsal light reflex (DLR), evaluates swimming angle based on input coming from above. OKR can used study effect acuity improvement recovery after rejuvenation therapy injury disease, whereas DLR best assess functional repair unilateral optic nerve crush.

Language: Английский

Citations

2