Orally delivered toxin–binding protein protects against diarrhoea in a murine cholera model

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 19, 2025

Abstract The ongoing seventh cholera pandemic, which began in 1961, poses an escalating threat to public health. There is a need for new control measures, particularly ones that can be produced at low cost, the one billion people living cholera-endemic regions. Orally delivered V H Hs, functioning as target-binding proteins, have been proposed potential approach gastrointestinal pathogens. Here, we describe development of orally deliverable bivalent construct binds B-pentamer toxin, showing it inhibits toxin activity murine challenge model. Infant mice given prior V. cholerae infection exhibit significant reduction toxin–associated intestinal fluid secretion and diarrhoea. In addition, reduces colonization levels small intestine by factor 10. This toxin–binding protein holds promise protecting against severe diarrhoea associated with cholera.

Language: Английский

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Allergenicity, Genotoxicity and Subchronic Toxicity Assessment of IgG Binding Protein LT Produced From Aspergillus oryzae

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

ABSTRACT Gastrointestinal health is one of the fastest growing areas in food and beverage industry, as its importance to overall well‐being becoming increasingly recognized. Immunoglobulins play a key role protecting gastrointestinal tract, nonbovine sources immunoglobulins (including camel milk, which has long history consumption East Africa Asia) are increasing popularity Western countries functional foods, particularly for individuals with allergies or intolerances cow's milk. The physiological benefits consuming certain heavy‐chain from milk relate binding domains camelid single‐domain antibodies; thus, novel protein termed “immunoglobulin G (IgG) LT” (a dimer two antibody sequences) been developed use products, provide some attributed on an industrial scale. To support safety IgG LT such use, comprehensive assessment (in silico allergenicity assessment, vitro genotoxicity studies [bacterial reverse mutation test mammalian cell micronucleus test], 90‐day gavage toxicity study rats) was conducted. results demonstrate that highly unlikely pose risk allergenic cross‐reactivity, there no evidence vitro. There were article–related effects study. These data intended uses foods beverages.

Language: Английский

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Effects of fermented unconventional protein feed on pig production in China

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 31, 2024

Unconventional protein feeds, characterized by low nutritional value, high variability, and poor palatability, have limited their application in swine production. Fermentation technology holds the key to addressing these shortcomings. Given ban on antibiotics China, inferior quality of imported pig breeds, long-term dependence soybean, prospects for fermented unconventional feeds are promising. This paper delves into common types factors influencing fermentation process, mechanisms which they enhance health, challenges offering theoretical insights future development feed industry.

Language: Английский

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Pharmacodynamic Evaluation of Phage Therapy in Ameliorating ETEC-Induced Diarrhea in Mice Models

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2532 - 2532

Published: Dec. 8, 2024

Enterotoxigenic Escherichia coli (ETEC) is a major pathogen causing diarrhea in humans and animals, with increasing antimicrobial resistance posing growing challenge recent years. Lytic bacteriophages (phages) offer targeted environmentally sustainable approach to combating bacterial infections, particularly eliminating drug-resistant strains. In this study, ETEC strains were utilized as indicators, stable, high-efficiency phage, designated vB_EcoM_JE01 (JE01), was isolated from pig farm manure. The genome of JE01 dsDNA molecule, measuring 168.9 kb, transmission electron microscope revealed its characteristic T4-like Myoviridae morphology. effectively lysed multi-drug-resistant isolates. Stability assays demonstrated that retained activity across temperature range 20 °C 50 pH 3–11, showing resilience ultraviolet radiation chloroform exposure. Furthermore, suppressed adhesion porcine intestinal epithelial cells (IPEC-J2), mitigating the inflammatory response triggered by ETEC. To investigate vivo antibacterial efficacy phage preparations, model established using germ-free mice infected strain. findings indicated 12 h post-ETEC inoculation, intragastric administration significantly reduced mortality, alleviated gastrointestinal lesions, decreased colonization jejunum, expression cytokines IL-6 IL-8. These results demonstrate therapeutic benefit treating ETEC-induced mice. Additionally, fluorescent incorporating red protein (RFP) engineered, pharmacokinetics therapy preliminarily assessed through fluorescence imaging showed localized jejunum rapidly, within 45 min. Moreover, markedly slowed presence target gut, suggesting sustained bacteriolytic ETEC-infected intestine. conclusion, study establishes foundation for development phage-based therapies against

Language: Английский

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Effect of second iron injection on growth performance, hematological parameters, and fecal microbiome of piglets fed different dietary iron levels

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 103

Published: Dec. 7, 2024

This experiment was conducted to evaluate the effects of a second iron injection for suckling pigs fed diets with different dietary levels in nursery period on growth performance, hematological parameters, serum and liver trace mineral content, fecal score, microbiome, metabolites. A total 70 newborn from 7 litters were assigned either 1 or 2 injections within litter received first i.m. (200 mg) at 3 d age. Pigs treatment an additional 5 after injection. At weaning (days 27 30 age), treatments divided into diet 27-d period. Treatments 1) no + 100 ppm (NC), 2) NC diets, 3) 200 (PC), 4) PC diets. The increased content (P = 0.08, tendency), iron, hemoglobin, hematocrit until day 13 postweaning < 0.05). In period, receiving had greater final body weight overall rate tendency) feed intake 0.05), lower score 0.05) indicating firmer feces compared those There major effect level interaction any measurements except that hemoglobin late than reduced bacterial alpha-diversity based Faith's phylogenetic diversity while resulted dissimilarity community Unweighted Unifrac analysis 0.05; by level). conclusion, improved performance affected whereas supplementation altered microbiome but did not affect growth.

Language: Английский

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