Precision Psychobiotics for Gut–Brain Axis Health: Advancing the Discovery Pipelines to Deliver Mechanistic Pathways and Proven Health Efficacy DOI Creative Commons
Rebecca F. Slykerman, Naomi Davies, Klára Vlčková

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

ABSTRACT Advancing microbiome–gut–brain axis science requires systematic, rational and translational approaches to bridge the critical knowledge gaps currently preventing full exploitation of gut microbiome as a tractable therapeutic target for gastrointestinal, mental brain health. Current research is still marked by many open questions that undermine widespread application humans. For example, lack mechanistic understanding probiotic effects means it remains unclear why even apparently closely related strains exhibit different in vivo. live microbial psychobiotics, consensus on their adjunct treatments conventional neuromodulators, use unmedicated populations or at‐risk cohorts with sub‐clinical symptomatology warranted. This missing information both sides equation when treating central nervous system (CNS) conditions makes psychobiotic challenging, especially compared other pharmaceutical functional food approaches. Expediting transition from positive preclinical data proven benefits humans includes interpreting promises pitfalls animal behavioural assays, well navigating mechanism‐informed decision making select right microbe(s) job. In this review, we consider how these decisions can be supported light accrued range clinical studies across healthy, pathological study populations, where specific have been evaluated context gastrointestinal physiology, function behaviour. Examples successful, partial unsuccessful translation bench bedside are considered. We also discuss developments silico analyses enhanced our moved towards pinpointing host–microbe interactions most important optimal gut–brain function. Combining assays vitro ex vivo domains incorporating model organisms prime discovery pipelines promising rationally selected candidates.

Language: Английский

Modulation of gut microbiome in the treatment of neurodegenerative diseases: A systematic review DOI Creative Commons

A. Mincic,

Miklós Antal, Lorena Filip

et al.

Clinical Nutrition, Journal Year: 2024, Volume and Issue: 43(7), P. 1832 - 1849

Published: May 31, 2024

Language: Английский

Citations

10
An optimized culturomics strategy for isolation of human milk microbiota DOI Creative Commons
Fan Wang,

Lingmin Yu,

Yuting Ren

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 1, 2024

Viable microorganisms and a diverse microbial ecosystem found in human milk play crucial role promoting healthy immune system shaping the community infant's gut. Culturomics is method to obtain comprehensive repertoire of microbiota. However, culturomics an onerous procedure, needs expertise, making it difficult be widely implemented. Currently, there no efficient feasible specifically designed for microbiota yet. Therefore, aim this study was develop more We obtained fresh samples from Chinese mothers conducted 27-day enrichment process using blood culture bottles. Bacterial isolates were harvested at different time intervals cultured on four types media. Using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we identified total 6601 colonies successfully 865 strains, representing 4 phyla, 21 genera, 54 species. By combining CBA MRS media, able cultivate over 94.4% bacterial species with high diversity, including species-specific microorganisms. Prolonged pre-incubation bottles significantly increased number by about 33% improved isolation efficiency beneficial bacteria low abundance milk. After optimization, reduced selected optimal picking time-points (0, 3, 6 days) 37°C. testing MALDI-TOF MS, estimated that new protocol could than 90% species, reducing workload 57.0%. In conclusion, our strategy, which involves combination extended enrichment, optimized time-points, studying This improves allows establishment strains

Language: Английский

Citations

9
Effects of microbiome-based interventions on neurodegenerative diseases: a systematic review and meta-analysis DOI Creative Commons
Zara Siu Wa Chui, Lily Man Lee Chan,

Esther Wan Hei Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 26, 2024

Abstract Neurodegenerative diseases (NDDs) are characterized by neuronal damage and progressive loss of neuron function. Microbiome-based interventions, such as dietary biotics, fecal microbiome transplant, have been proposed a novel approach to managing symptoms modulating disease progression. Emerging clinical trials investigated the efficacy interventions GM in alleviating or reversing progression, yet no comprehensive synthesis done. A systematic review literature was therefore conducted investigate microbiome-modulating methods. The search yielded 4051 articles, with 15 included. overall risk bias moderate most studies. Most changed composition. Despite inconsistent changes composition, meta-analysis showed that improved burden (SMD, − 0.57; 95% CI 0.93 0.21; I 2 = 42%; P 0.002) qualitative trend improvement constipation. However, current studies high methodological heterogeneity small sample sizes, requiring more well-designed controlled elucidate complex linkage between microbiome, NDDs.

Language: Английский

Citations

9
Gut Microbiota-Based Interventions for Parkinson’s Disease: Neuroprotective Mechanisms and Current Perspective DOI
Deepak Kumar, Mahendra Bishnoi, Kanthi Kiran Kondepudi

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Language: Английский

Citations

1
Precision Psychobiotics for Gut–Brain Axis Health: Advancing the Discovery Pipelines to Deliver Mechanistic Pathways and Proven Health Efficacy DOI Creative Commons
Rebecca F. Slykerman, Naomi Davies, Klára Vlčková

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

ABSTRACT Advancing microbiome–gut–brain axis science requires systematic, rational and translational approaches to bridge the critical knowledge gaps currently preventing full exploitation of gut microbiome as a tractable therapeutic target for gastrointestinal, mental brain health. Current research is still marked by many open questions that undermine widespread application humans. For example, lack mechanistic understanding probiotic effects means it remains unclear why even apparently closely related strains exhibit different in vivo. live microbial psychobiotics, consensus on their adjunct treatments conventional neuromodulators, use unmedicated populations or at‐risk cohorts with sub‐clinical symptomatology warranted. This missing information both sides equation when treating central nervous system (CNS) conditions makes psychobiotic challenging, especially compared other pharmaceutical functional food approaches. Expediting transition from positive preclinical data proven benefits humans includes interpreting promises pitfalls animal behavioural assays, well navigating mechanism‐informed decision making select right microbe(s) job. In this review, we consider how these decisions can be supported light accrued range clinical studies across healthy, pathological study populations, where specific have been evaluated context gastrointestinal physiology, function behaviour. Examples successful, partial unsuccessful translation bench bedside are considered. We also discuss developments silico analyses enhanced our moved towards pinpointing host–microbe interactions most important optimal gut–brain function. Combining assays vitro ex vivo domains incorporating model organisms prime discovery pipelines promising rationally selected candidates.

Language: Английский

Citations

1