New insights into the cultivability of human milk microbiota from ingestion to digestion and implications for its immunomodulatory properties DOI Creative Commons

Charles Le Bras,

Alizé Mouchard,

Lucie Rault

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Human milk (HM) microbiota is increasingly studied for its potential health benefits. However, the physiological state of HM bacteria and consequently their effects on gut homeostasis remain a question. This study investigated by characterizing cultivable fraction, as it might be at point ingestion assessing digestion cultivability immunomodulatory properties six prevalent strains. The 28 samples was analysed 16S metabarcoding either directly raw (raw microbiota, RM) or complete fraction obtained from seven non-selective media (cultivable CM). Diversity lower in CM than RM, with 32 versus 435 genera median 7 69 per sample respectively. also showed under-representation strictly anaerobic genera. Factors like parity iron vitamin supplementation affected RM and/or CM. In vitro gastrointestinal moderately impacted strain cultivability. most strains partially completely lost monocyte THP1 cell line after digestion, except Staphylococcus epidermidis that gained potential.

Language: Английский

Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications DOI Creative Commons
Rine Christopher Reuben, Cármen Torres

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Jan. 18, 2025

Mammalian milk contains a variety of complex bioactive and nutritional components microorganisms. These microorganisms have diverse compositions functional roles that impact host health disease pathophysiology, especially mastitis. The advent use high throughput omics technologies, including metagenomics, metatranscriptomics, metaproteomics, metametabolomics, as well culturomics in microbiome studies suggest strong relationships between phenotype signatures While single undoubtedly contributed to our current understanding mastitis, they often provide limited information, targeting only biological viewpoint which is insufficient system-wide information necessary for elucidating the footprints molecular mechanisms driving mastitis dysbiosis. Therefore, integrating multi-omics approach research could generate new knowledge, improve structural ecosystem, insights sustainable control management.

Language: Английский

Citations

1
A comprehensive review of the characterization, host interactions, and stabilization advancements on probiotics: Addressing the challenges in functional food diversification DOI Creative Commons
Eskindir Getachew Fentie,

Kyeongmo Lim,

Minsoo Jeong

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(5)

Published: Aug. 13, 2024

The demand for functional food is rising in tandem with the prevalence of chronic diseases. Probiotics play a crucial role development, yet their ability to confer health benefits host remains topic debate according Food and Agriculture Organization/World Health Organization requirements. application culturomics, innovative isolation techniques, within realm probiotics increasingly deemed essential fully harnessing latent potential microbial reservoirs. Nevertheless, its confined predominantly human fecal sources. Following integration probiogenomics, significant advancements have been made safety assessment probiotics. However, adoption novel probiotic microorganisms has match requisite pace. Progress research concerning host-probiotic interactions by employing omics technologies, particularly animal models, notable. Nonetheless, comprehensive elucidation mechanisms action trial studies are lagging behind. Additionally, viability probiotics, spanning from production as foods transit colon, markedly improved through encapsulation techniques. opportunities exploration persist regarding alternative coating materials diverse methodologies. Furthermore, there discernible transition domain probiotic-based foods, shifting away primarily dairy-centric focus toward inclusion broader array categories. This review addresses critical issues ranging sources techniques final developments. while doing so, it explores probiogenomics applications characterization, investigations into interactions, strategies stabilization under harsh environmental conditions.

Language: Английский

Citations

4
Relationship Between Human Microbiome and Helicobacter pylori DOI Creative Commons

Giada La Placa,

Marcello Covino,

Marcello Candeli

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(1), P. 24 - 24

Published: Jan. 17, 2025

The enteric microbiota influences gene expression in the colonic epithelium. H. pylori (Helicobacter pylori) affects gastric growth factors, cytokines, and tumor cell markers, potentially benefiting host. interaction between human is complex but appears beneficial disease development. This publication discusses microbiota, microbiome, chronic colonization, bacterium’s role disease. interplay bacterium during infection also explored. gut has most abundant microbial community performs vital roles food digestion nutrient uptake, extraction of absorbable vitamins, inhibition pathogen colonization. Helicobacter a highly specialized pathogen, predominantly colonizing mucus layer, on or adherent to underlying epithelial surfaces. Many studies have suggested that microbiome related infection, some bacterial species can be used for detection diagnosis infection. Human genome analysis revolutionized our understanding intricate relationship humans their associated communities. integration data displayed promising capabilities occult detection, unveiling previously hidden pathologies providing opportunities early intervention. These groundbreaking discoveries paved way novel therapeutic strategies deeper comprehension interconnected nature overall health.

Language: Английский

Citations

0
New insights into the cultivability of human milk bacteria from ingestion to digestion and implications for their Immunomodulatory properties DOI Creative Commons

Charles Le Bras,

Alizé Mouchard,

Lucie Rault

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 31, 2025

Abstract Human milk (HM) microbiota is increasingly studied for its potential health benefits. However, the physiological state of HM bacteria and consequently their effects on gut homeostasis remain a question. This study investigated by characterizing cultivable fraction as it might be at point ingestion assessing digestion, in specific context immature infant digestive tract, cultivability immunomodulatory properties six strains representative prevalent genera HM. Twenty-eight samples were analysed 16 S metabarcoding either directly raw (raw microbiota, RM) or complete obtained from seven non-selective media (cultivable CM). approach enabled more in-depth investigation CM than conventional methods based individual sequencing subset isolates resulted moderate gain diversity within each sample. It confirmed that was lower RM, with ~ 7 versus 69 per sample RM respectively, an under-representation strictly anaerobic CM. In vitro gastrointestinal digestion overall good survival 6 but partial loss monocyte THP1 cell line, except Staphylococcus epidermidis strain gained potential. These results highlight to survive during interact intestinal epithelium immune system, well importance considering process when evaluating host-bacteria interactions.

Language: Английский

Citations

0
Metagenomic and culturomic analyses of bacterial species contributing to tyramine formation in Cheonggukjang DOI Creative Commons
Jong Hyoung Hong, Young Hun Jin, Alixander Mattay Pawluk

et al.

LWT, Journal Year: 2024, Volume and Issue: 201, P. 116265 - 116265

Published: May 28, 2024

This study investigated bacterial compositions and their contributions to biogenic amine (BA) formation in Cheonggukjang using metagenomic culturomic analyses. Metagenomic analysis revealed Bacillus spp. as dominant (96.19%), of which B. piscis (74.79%) hisashii (19.39%) were predominant. Based on the relative abundance BA-associated genes, showed highest correlations with tyrosine (R2 = 0.76) lysine decarboxylation 0.50), histidine 0.42). Meanwhile, that, among mesophilic bacteria, (98.78%). As lactic acid bacteria (LAB), only Enterococcus detected. Of halotolerant LAB, (90.72%). vitro BA productivity all strains, contributed primarily tyramine formation. Thus, it was observed that determined two analyses differed considerably. To verify such observations, fermentation experiments conducted strains. The content higher Bacillus-fermented samples (over 120.00 ± 14.18 mg/kg) than Enterococcus-fermented sample (34.24 3.37 mg/kg). However, spermidine spermine (108.04 6.69 22.93 6.28 mg/kg, respectively). Therefore, these species may contribute together but slightly different manners.

Language: Английский

Citations

2
Identification and Characterization of Human Breast Milk and Infant Fecal Cultivable Lactobacilli Isolated in Bulgaria: A Pilot Study DOI Creative Commons

Asya Asenova,

Hristiyana Hristova,

Stanimira Ivanova

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1839 - 1839

Published: Sept. 5, 2024

During the last few decades, main focus of numerous studies has been on human breast milk microbiota and its influence infant intestinal overall health. The presence lactic acid bacteria in affects both quantitative qualitative composition gut microbiota. aim this study was to assess most frequently detected cultivable rod-shaped lactobacilli, specific for healthy Bulgarian women fecal samples their infants over first month life, 14 mother-infant tandem pairs. Additionally, we evaluated strain diversity among common isolated species. A total 68 Gram-positive catalase-negative strains were subjected identification using MALDI-TOF technique. Predominant populations belonging have identified as

Language: Английский

Citations

2
Culturomics: A Promising Approach for Exploring Bacterial Diversity in Natural Fermented Milk DOI
Yu Li, Xiaoqing Guo, Q. Y. Peng

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105383 - 105383

Published: Oct. 1, 2024

Language: Английский

Citations

1
Function-driven high-throughput screening and isolation of ester-producing strains for glutinous rice wine fermentation DOI
Chong Chen, Zhaojun Zheng, Yue Wang

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 199, P. 115393 - 115393

Published: Nov. 24, 2024

Language: Английский

Citations

1
New insights into the cultivability of human milk microbiota from ingestion to digestion and implications for its immunomodulatory properties DOI Creative Commons

Charles Le Bras,

Alizé Mouchard,

Lucie Rault

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Human milk (HM) microbiota is increasingly studied for its potential health benefits. However, the physiological state of HM bacteria and consequently their effects on gut homeostasis remain a question. This study investigated by characterizing cultivable fraction, as it might be at point ingestion assessing digestion cultivability immunomodulatory properties six prevalent strains. The 28 samples was analysed 16S metabarcoding either directly raw (raw microbiota, RM) or complete fraction obtained from seven non-selective media (cultivable CM). Diversity lower in CM than RM, with 32 versus 435 genera median 7 69 per sample respectively. also showed under-representation strictly anaerobic genera. Factors like parity iron vitamin supplementation affected RM and/or CM. In vitro gastrointestinal moderately impacted strain cultivability. most strains partially completely lost monocyte THP1 cell line after digestion, except Staphylococcus epidermidis that gained potential.

Language: Английский

Citations

0