Combined biolistic and cell penetrating peptide delivery for the development of scalable intradermal DNA vaccines DOI Creative Commons

Roizza Beth So,

Gang Li, Victoria A. Brentville

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 367, P. 209 - 222

Published: Jan. 26, 2024

Physical-based gene delivery via biolistic methods (such as the Helios gun) involve precipitation of nucleic acids onto microparticles and direct transfection through cell membranes exposed tissue (e.g. skin) by high velocity acceleration. The glycosaminoglycan (GAG)-binding enhanced transduction (GET) system exploits novel fusion peptides consisting cell-binding, acid condensing, cell-penetrating domains, which enable across multiple types. In this study, we combined chemical physical (gene DNA systems, hypothesized combination would generate distribution effective uptake in cells not initially transfected penetration. Physicochemical characterization, optimization bullet contents experiments vitro monolayers engineered demonstrated these formulations efficiently, including DC2.4 dendritic cells. We incorporated into a format for gun forming fireable dry bullets obtained lyophilization (freeze drying). This is simple with scalability compared to conventional bullets. Flushed GET retained their ability mediate (17-fold greater 13-fold reporter expression than standard spermidine absence presence serum, respectively). Fired (in collagen gels) vivo (mice) showed increased untreated controls, whilst maintaining viability having no obvious toxicity vivo. Lastly, SARS-CoV-2 plasmid vaccine spike (S) protein-receptor binding domain (S-RBD) was delivered using Specific T antibody responses comparable were generated. non-physical GET‑gold-DNA carriers shows potential an alternative method that scalable mass deployable vaccination intradermal delivery.

Language: Английский

Adenoviral vectored vaccination protects against Crimean-Congo Haemorrhagic Fever disease in a lethal challenge model DOI Creative Commons
Jack E. Saunders, Ciarán Gilbride, Stuart Dowall

et al.

EBioMedicine, Journal Year: 2023, Volume and Issue: 90, P. 104523 - 104523

Published: March 17, 2023

Language: Английский

Citations

22
An Old Acquaintance: Could Adenoviruses Be Our Next Pandemic Threat? DOI Creative Commons
Gustavo Saint-Pierre, Daniel Conei,

Luis Lizama

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(2), P. 330 - 330

Published: Jan. 24, 2023

Human adenoviruses (HAdV) are one of the most important pathogens detected in acute respiratory diseases pediatrics and immunocompromised patients. In 1953, Wallace Rowe described it for first time oropharyngeal lymphatic tissue. To date, more than 110 types HAdV have been described, with different cellular tropisms. They can cause gastrointestinal symptoms, even urinary tract inflammation, although infections asymptomatic. However, there is a population at risk that develop serious lethal conditions. These viruses double-stranded DNA genome, 25–48 kbp, 90 nm diameter, without mantle, stable environment, resistant to fat-soluble detergents. Currently diagnosis made lateral flow immunochromatography or molecular biology through polymerase chain reaction. This review aimed highlight variability pandemic potential HAdV3 7 recombinant could considering aggressive outbreaks produced health facilities. Herein, we characteristics HAdV, from infection treatment, vaccine development, evaluation social determinants associated suggesting necessary measures future sanitary control prevent disasters such as SARS-CoV-2 pandemic, an emphasis on use AdV vaccines other pandemics.

Language: Английский

Citations

20
Vaccine development for mosquito-borne viral diseases DOI Creative Commons
Zhiwei Huang, Yuxuan Zhang, Hongyu Li

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 12, 2023

Mosquito-borne viral diseases are a group of illnesses that predominantly transmitted by mosquitoes, including viruses from the Togaviridae and Flaviviridae families. In recent years, outbreaks caused Dengue Zika family, Chikungunya virus have raised significant concerns for public health. However, there currently no safe effective vaccines available these viruses, except CYD-TDV, which has been licensed virus. Efforts to control transmission COVID-19, such as home quarantine travel restrictions, somewhat limited spread mosquito-borne diseases. Several vaccine platforms, inactivated vaccines, viral-vector live attenuated protein nucleic acid being developed combat viruses. This review analyzes various platforms against Dengue, Zika, provides valuable insights responding potential outbreaks.

Language: Английский

Citations

18
The Upper Nasal Space: Option for Systemic Drug Delivery, Mucosal Vaccines and “Nose-to-Brain” DOI Creative Commons
Stephen B. Shrewsbury

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1720 - 1720

Published: June 13, 2023

Sino-nasal disease is appropriately treated with topical treatment, where the nasal mucosa acts as a barrier to systemic absorption. Non-invasive delivery of drugs has produced some small molecule products good bioavailability. With recent COVID pandemic and need for mucosal immunity becoming more appreciated, interest become focused on cavity vaccine delivery. In parallel, it been recognized that drug different parts nose can have results “nose-to-brain” delivery, deposition olfactory epithelium upper space desirable. Here non-motile cilia reduced mucociliary clearance lead longer residence time permits enhanced absorption, either into circulation or directly CNS. Many developments in add bioadhesives absorption/permeation enhancers, creating complicated formulations development pathways, but other projects shown device itself may allow differential targeting without these additions could faster efficient programs bring wider range drugs—and vaccines—to market.

Language: Английский

Citations

18
Combined biolistic and cell penetrating peptide delivery for the development of scalable intradermal DNA vaccines DOI Creative Commons

Roizza Beth So,

Gang Li, Victoria A. Brentville

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 367, P. 209 - 222

Published: Jan. 26, 2024

Physical-based gene delivery via biolistic methods (such as the Helios gun) involve precipitation of nucleic acids onto microparticles and direct transfection through cell membranes exposed tissue (e.g. skin) by high velocity acceleration. The glycosaminoglycan (GAG)-binding enhanced transduction (GET) system exploits novel fusion peptides consisting cell-binding, acid condensing, cell-penetrating domains, which enable across multiple types. In this study, we combined chemical physical (gene DNA systems, hypothesized combination would generate distribution effective uptake in cells not initially transfected penetration. Physicochemical characterization, optimization bullet contents experiments vitro monolayers engineered demonstrated these formulations efficiently, including DC2.4 dendritic cells. We incorporated into a format for gun forming fireable dry bullets obtained lyophilization (freeze drying). This is simple with scalability compared to conventional bullets. Flushed GET retained their ability mediate (17-fold greater 13-fold reporter expression than standard spermidine absence presence serum, respectively). Fired (in collagen gels) vivo (mice) showed increased untreated controls, whilst maintaining viability having no obvious toxicity vivo. Lastly, SARS-CoV-2 plasmid vaccine spike (S) protein-receptor binding domain (S-RBD) was delivered using Specific T antibody responses comparable were generated. non-physical GET‑gold-DNA carriers shows potential an alternative method that scalable mass deployable vaccination intradermal delivery.

Language: Английский

Citations

8