A review on Zika vaccine development

Pathogens and Disease, Journal Year: 2024, Volume and Issue: 82

Published: Jan. 1, 2024

Abstract Zika virus (ZIKV), which belongs to the Flavivirus family, is mainly transmitted via bite of Aedes mosquitoes. In newborns, ZIKV infection can cause severe symptoms such as microcephaly, while in adults, it lead Guillain‒Barré syndrome (GBS). Due lack specific therapeutic methods against ZIKV, development a safe and effective vaccine extremely important. Several potential vaccines, live attenuated, inactivated, nucleic acid, viral vector, recombinant subunit have demonstrated promising outcomes clinical trials involving human participants. Therefore, this review, recent developmental progress, advantages disadvantages these five types are examined, practical recommendations for future provided.

Language: Английский

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Development of Vaccines against Emerging Mosquito-Vectored Arbovirus Infections

Vaccines, Journal Year: 2024, Volume and Issue: 12(1), P. 87 - 87

Published: Jan. 15, 2024

Among emergent climate-sensitive infectious diseases, some mosquito-vectored arbovirus infections have epidemiological, social, and economic effects. Dengue virus (DENV), West Nile (WNV), Chikungunya (CHIKV) disease, previously common only in the tropics, currently pose a major risk to global health are expected expand dramatically near future if adequate containment measures not implemented. The lack of safe effective vaccines is critical as it seems likely that emerging will be con-trolled when against each these become available. This paper discusses clinical characteristics DENV, WNV, CHIKV state development viruses. An ideal vaccine should able evoke with single administration prompt activation B T cells, concentrations protecting/neutralizing antibodies, creation strong immune memory capable triggering an secondary antibody response after new infection wild-type and/or mutated agent. Moreover, well tolerated, safe, easily administrated, cost-effective, widely available throughout world. However, diseases far from being satisfactory, take many years before for all made worldwide.

Language: Английский

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Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Journal of Pest Science, Journal Year: 2024, Volume and Issue: 97(4), P. 2203 - 2215

Published: March 21, 2024

Abstract Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes known to produce array natural products with various activities including larvicidal activity. In this study, the effects produced four one bacteria ovipositional behavior albopictus mosquitoes were assessed. Utilizing a binary choice assay insect cages, gravid female presented two oviposition cups containing water supplemented varying concentrations supernatants (50–1% concentrations) versus control medium. After 72 h, eggs deposited filter papers counted. The attractant index (OAI) feature supernatant was evaluated using number laid cups. Notably, all tested exhibited concentration-dependent deterrence oviposition. cabanillasii displayed strongest deterrent effect, inhibiting egg-laying at 50–5% (OAI: − 0.87 0.35), followed X. nematophila (50–10%, OAI: 0.82 0.52). szentirmaii, doucetiae, P. kayaii showed significant ≥ 20% concentrations. Using promoter exchange mutants generated easyPACId approach, fabclavine from szentirmaii identified bioactive compound evident effects. Such deterrents targeting could valuable for controlling populations disrupting their breeding suitable habitats.

Language: Английский

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Synthesis and Biological Evaluation of 1,2-Diborane Derivatives as Mosquito Larvicides

Published: Jan. 1, 2025

Language: Английский

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Lysinibacillus sphaericus exposure impedes Anopheles dirus’s oviposition via downregulating vitellogenin

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 21, 2025

Abstract Background Vector control using Lysinibacillus sphaericus is an effective strategy for preventing the transmission of mosquito-borne diseases. Our previous study demonstrated that exposure to L. during larval stage Anopheles dirus significantly reduced fecundity surviving adult mosquitoes. However, underlying mechanisms driving this reduction remain unclear. Sublethal doses , often resulting from insufficient or delayed application, can still impact mosquito populations. Therefore, aimed investigate how sublethal inhibit reproductive capacity An. Methods First, staining method was used detect in mosquitoes had been exposed stage. Second, were fed a sucrose solution containing and effects on observed. Third, transcriptome sequencing qPCR employed identify validate genes associated with oviposition suppression following treatment . Finally, we assessed direct feeding vitellogenin ( Vg ) expression activation target rapamycin (TOR) signaling pathway Western blotting. Results findings persists An survived doses. Additionally, female suppressed their ability. Transcriptome analysis revealed substantial alterations gene profiles among Notably, lysosomal function lipid metabolism, which are critical physiology. Furthermore, downregulated Akt-TOR Conclusions Exposure larvae resulted persistence by downregulating via inhibition TOR pathway. This offers novel insights into interaction between its host identifies potential molecular targets controlling population density modulating behavior. Graphical

Language: Английский

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Fighting RNA viruses with a gold nanoparticle Cas13d gene-editing armor

Molecular Therapy — Nucleic Acids, Journal Year: 2025, Volume and Issue: 36(2), P. 102540 - 102540

Published: April 17, 2025

A novel Cas13d-based gene-editing approach has been developed to target viral RNAs in infected cells, reducing the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Zika virus (ZIKV) by up 90% compared with controls. Despite its potential, use Cas13d as an antiviral faces several challenges that limit effectiveness before reaching cells. This study presents a proof-of-concept strategy for constructing gold nanoparticles (Au_NPs) destroy SARS-CoV-2 ZIKV genomes into The Au_NPs complexes were administered Huh-7 cells either virus, single or multiple doses. demonstrated cuts comparable efficiency lipofected ribonucleoprotein (RNP). Additionally, we found can spontaneously enter endocytosis diffusion, first 4 h treatment. co-localized virions early endosomes reduced after administration, unlike RNPs, which showed no activity. However, was less efficient at Cas13d-RNPs, likely due different intracellular localization. These results suggest be adapted new strategy, highlighting innovative delivery method against viruses without need transfecting, providing gene-editing-based emerging RNA viruses.

Language: Английский

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The current status of arboviruses with major epidemiological significance in Europe

Enfermedades Infecciosas y Microbiología Clínica, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

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A novel pan-epitope based nanovaccine self-assembled with CpG enhances immune responses against flavivirus

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 28, 2024

Flavivirus is a highly prevalent and outbreak-prone disease, affecting billions of individuals annually posing substantial public health challenges. Vaccination critical to reducing the global impact flavivirus infections, making development safe effective vaccine top priority. The self-assembled pan-epitope presents key advantages for improving immunogenicity safety without relying on external vectors or adding immunomodulatory elements, both which are essential successful development. In this study, peptide TBT was combined with adjuvant CpG form TBT-CpG nanovaccine (TBT-CpG NaVs), found be spherical, uniform in shape, demonstrated strong serum stability. vitro studies showed that NaVs were efficiently taken up internalized by bone marrow-derived dendritic cells (BMDCs). Flow cytometry transcriptomic analysis indicated antigens effectively presented antigen-presenting (APCs) via MHC II pathway, facilitated BMDCs maturation promoted release pro-inflammatory cytokines IL-1β, TNF-α, IL-6. vivo confirmed enhanced antigen-specific IgG levels, significantly increased IFN-γ IL-4 expression spleen cells, offered protective effects against Dengue virus (DENV) Zika (ZIKV) infections. Safety evaluations revealed no hepatotoxicity significant organ damage immunized mice. candidate activates triggers targeted immune response, providing antiviral DENV ZIKV. This demonstrates good safety, establishing promising foundation new strategy vaccines.

Language: Английский

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Trends and insights in dengue virus research globally: a bibliometric analysis (1995–2023)

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 3, 2024

Dengue virus (DENV) is the most widespread arbovirus. The World Health Organization (WHO) declared dengue one of top 10 global health threats in 2019. However, it has been underrepresented bibliometric analyses. This study employs analysis to identify research hotspots and trends, offering a comprehensive overview current dynamics this field. We present report spanning from 1995 2023 that provides unique longitudinal research, revealing significant trends shifts not extensively covered previous literature. A total 10,767 DENV-related documents were considered, with notable increase publications, peaking at 747 articles 2021. Plos Neglected Tropical Diseases become leading journal publishing 791 field—the highest number recorded. Our mapping DENV across multiple dimensions, including vector ecology, virology, emerging therapies. delineates complex network immune response genes, IFNA1, DDX58, IFNB1, STAT1, IRF3, NFKB1, highlighting themes, particularly impacts climate change new outbreaks on disease transmission. findings detail progress status key vaccine candidates, licensed Dengvaxia, newer vaccines such as Qdenga TV003, updated clinical trials. underscores advancements antiviral therapies control strategies for dengue, innovative drug candidates AT-752 JNJ-1802, potential repurposing agents like Ribavirin, Remdesivir, Lopinavir. Additionally, discusses biological methods, introduction Wolbachia-infected mosquitoes gene-editing technologies. critical role interdisciplinary collaboration advancing identifying areas needing further exploration, host-virus dynamics, development application drugs vaccines, use artificial intelligence. It advocates strengthened partnerships various disciplines effectively tackle challenges posed by DENV.

Language: Английский

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Unleashing Nature’s Allies: Comparing the Vertical Transmission Dynamics of Insect-Specific and Vertebrate-Infecting Flaviviruses in Mosquitoes

Viruses, Journal Year: 2024, Volume and Issue: 16(9), P. 1499 - 1499

Published: Sept. 23, 2024

Insect-specific viruses (ISVs) include that are restricted to the infection of mosquitoes and spread mostly through transovarial transmission. Despite using a distinct mode transmission, ISVs often phylogenetically related arthropod-borne (arboviruses) responsible for human diseases able infect both vertebrates. can also induce phenomenon called "superinfection exclusion", whereby primary ISV in an insect inhibits subsequent viral infections insect. This has sparked interest use control pathogenic arboviruses transmitted by mosquitoes. In particular, insect-specific flaviviruses (ISFs) have been shown inhibit vertebrate-infecting (VIFs) vitro vivo. potential as new ecologically friendly biological approach arboviral disease. For this intervention lasting impacts control, it is imperative ISFs maintained mosquito populations with high rates vertical Therefore, these strategies will need optimise transmission order establish persistently infected lines sustainable arbovirus control. review compares recent observations determinants understand how may be optimised effective

Language: Английский

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