Computers in Biology and Medicine, Journal Year: 2024, Volume and Issue: 173, P. 108325 - 108325
Published: March 16, 2024
Language: Английский
Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(12), P. 2085 - 2166
Published: Jan. 1, 2024
This comprehensive review summarizes the past, present, and future of plasmonic NP-based sensors in terms different sensing platforms, chemical biological analytes, expected technologies.
Language: Английский
Citations
18
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 486, P. 150224 - 150224
Published: March 6, 2024
Language: Английский
Citations
8
Nanoscale, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Current molecular tests for tuberculosis (TB), such as whole genome sequencing and Xpert Mycobacterium tuberculosis/rifampicin resistance assay, exhibit limited sensitivity necessitate the pre-amplification step of target DNA. This limitation greatly increases detection time poses an increased risk infection. Here, we present a graphene field-effect transistor (GFET) based on CRISPR/Cas system detecting tuberculosis. The CRISPR/Cas12a has ability to specifically recognize cleave By integrating onto FET platform utilizing its electrical amplification capability, achieve rapid sensitive without requiring sample pre-amplification, with limit (LoD) low 2.42 × 10-18 M. Cas12a-GFET devices can differentiate 30 positive cases from 56 serum samples within 5 minutes. These findings highlight immense potential in future biological analysis clinical diagnosis.
Language: Английский
Citations
1
EBioMedicine, Journal Year: 2025, Volume and Issue: 112, P. 105564 - 105564
Published: Jan. 24, 2025
Language: Английский
Citations
1
Aggregate, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 6, 2025
ABSTRACT Metal‐enhanced fluorescence (MEF) represents a captivating phenomenon that transpires when fluorophores are situated in close vicinity to the surface of metallic nanostructures, leading nuanced augmentation their fluorescent characteristics. Given its efficacy enhancing excitation rates, quantum yield, and photostability, MEF has firmly established itself as highly valuable tool for augmenting biosensor sensitivity, bioimaging clarity, intensifying therapeutic responses. Notably, plasmonic gold inherently advantageous MEF, have been widely utilized signal amplification, labeling, theranostics. In this endeavor, we undertake comprehensive examination MEF‐enabled meticulously analyzing fundamental enhancement mechanisms, crucial influencing factors, diverse modes enhancement. Furthermore, spotlight exemplary applications these nanostructures biosensing, bioimaging, theranostics, underscoring revolutionary potential. Ultimately, offer glimpse into future prospects improvement challenges beset gold‐based MEF. Our attention is steadfastly directed toward essential scientific questions technical hurdles remain be surmounted, inviting readers partake an exciting exploration dynamic promising field.
Language: Английский
Citations
1
ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(9), P. 10660 - 10669
Published: April 23, 2024
There is an urgent need for efficient and convenient methods to detect SARS-CoV-2 as the global pandemic continues. Currently, detection of COVID-19 relies primarily on reverse transcription PCR (RT-PCR), a laboratory-based method that time-consuming. To tackle this challenge, study proposes immediate high-throughput platform SARS-CoV-2. The uses gold nanoenzymatic colorimetric assay focuses specific 3CLpro in has been extensively characterized stability, sensitivity, range, response time. not only provides easy-to-read results but also allows color readings be taken using smartphone, with accuracy comparable large instruments such microplate reader. innovation technology lies ability achieve instant detection, which makes rapid feasible. proposed wide range potential applications, particularly field virus providing reliable solution managing current pandemics future disease prevention control.
Language: Английский
Citations
7
Talanta, Journal Year: 2024, Volume and Issue: 278, P. 126463 - 126463
Published: June 25, 2024
Language: Английский
Citations
7
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 16, 2024
Abstract The unmet on‐site clinical diagnostics are increasingly demanding portable bioassays, but compromised by sensitivity and throughput. Herein, a nanostructured bubbles‐enhanced fluorescence (nBEF) assay is reported interfacing with smartphone imaging system to enable portable, ultrasensitive, specific miRNA detection in parallel. In nBEF, the high‐curvature bubbles allow physically enhancing optical propagation, chemically fluorescence, assembling more DNA probes, undergoing faster self‐suspension efficient self‐aggregation enhancement relative their smooth counterparts. By coupling bubble‐driven signal amplification plug‐and‐play concatemer, system, this nBEF achieves ultrasensitive fluorescent of at femtomolar level one‐base resolution single test, capable monitoring drug‐induced dynamic expression accurate cancer diagnosis (AUC = 1) through profiling 40 plasma samples after RNA extraction. This further integrates an R‐value analysis RGB module realize automated, readout. Such high‐performance provides low‐cost, highly sensitive tool diagnosis.
Language: Английский
Citations
4
Small Methods, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 2, 2025
Abstract The rapid, precise, and automated diagnosis of infectious diseases is crucial for effective disease management control. Herein, the integrated portable automatic digital detection system (IPADS), a novel diagnostic platform nucleic acid introduced. device employs hybrid magnetic (HMS), which uses an electromagnet movable permanent magnet to modulate field control bead movement, increasing extraction efficiency over 80%, while simplifying traditional labor‐intensive process enabling quick, low‐risk sample processing. Additionally, disposable cartridge designed HMS based preprocessing, with performed using RPA‐Cas12a, enclosed, automation‐friendly across dynamic range spanning five orders magnitude, sensitivity as low 100 copies mL −1 in serum samples. An further optimizes workflow. As proof concept, IPADS applied detect hepatitis B virus (HBV) DNA 20 clinical samples, demonstrating high concordance gold‐standard quantitative PCR (qPCR) methods. These results validate potential reliable point‐of‐care testing solution.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 16, 2025
Abstract During the COVID‐19 pandemic, reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) has been recognized as most reliable diagnostic tool. However, there is a need to develop multiplexed assays capable of analyzing multiple genes simultaneously expand its application. To address this, RT‐qPCR using double emulsion (DE)‐based carrier and polymer microparticle reactor, termed primer‐incorporated network tailored with Taqman probe (TaqPIN) developed. The DE securely stores nucleic acid reagents like primers probes within until heating releases them for reaction. TaqPIN demonstrates an amplification efficiency 93.8% can detect few 20 copies/µL. By loading microparticles into single reaction, assay only one optical channel enabled. In practice, nine‐plex designed distinguish between variants severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Even subtle variations nucleotide be detected. Testing on 75 nasopharyngeal swab samples yields 100% sensitivity specificity SARS‐CoV‐2 detection 94% accuracy in variant discrimination.
Language: Английский
Citations
0