Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(9), P. 5397 - 5434

Published: Jan. 1, 2021

Nanoparticles (NPs) have attracted considerable attention in various fields, such as cosmetics, the food industry, material design, and nanomedicine. In particular, fast-moving field of nanomedicine takes advantage features NPs for detection treatment different types cancer, fibrosis, inflammation, arthritis well neurodegenerative gastrointestinal diseases. To this end, a detailed understanding NP uptake mechanisms by cells intracellular localization is essential safe efficient therapeutic applications. first part review, we describe several endocytic pathways involved internalization discuss impact physicochemical properties on process. addition, potential challenges using inhibitors, markers genetic approaches to study endocytosis are addressed along with principal (semi) quantification methods uptake. The second focuses synthetic bio-inspired substances, which can stimulate or decrease cellular NPs. This approach could be interesting where high accumulation drugs target desirable clearance immune avoided. review contributes an improved reveals used improve delivery.

Language: Английский

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Single-molecule localization microscopy

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 3, 2021

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules are computationally localized from sequences localizations used to generate super-resolution or time course images, define trajectories. this Primer, we introduce basic principles SMLM before describing main experimental considerations when performing including labelling, sample preparation, hardware requirements acquisition in fixed live cells. We then explain how low-resolution processed reconstruct images and/or extract quantitative information, highlight selection discoveries enabled by closely related methods. discuss some limitations potential artefacts as well ways alleviate them. Finally, present an outlook on advanced promising new developments fast-evolving field SMLM. hope Primer will be useful reference for both newcomers practitioners

Language: Английский

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Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction

Nature Methods, Journal Year: 2019, Volume and Issue: 16(12), P. 1215 - 1225

Published: July 8, 2019

Language: Английский

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Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus

Molecular Cell, Journal Year: 2019, Volume and Issue: 76(5), P. 767 - 783.e11

Published: Sept. 17, 2019

Language: Английский

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Liquid–liquid phase separation drives cellular function and dysfunction in cancer

Nature reviews. Cancer, Journal Year: 2022, Volume and Issue: 22(4), P. 239 - 252

Published: Feb. 11, 2022

Language: Английский

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Nascent RNA analyses: tracking transcription and its regulation

Nature Reviews Genetics, Journal Year: 2019, Volume and Issue: 20(12), P. 705 - 723

Published: Aug. 9, 2019

Language: Английский

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Quantum-enhanced nonlinear microscopy

Nature, Journal Year: 2021, Volume and Issue: 594(7862), P. 201 - 206

Published: June 9, 2021

Language: Английский

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Roadmap of Terahertz Imaging 2021

Sensors, Journal Year: 2021, Volume and Issue: 21(12), P. 4092 - 4092

Published: June 14, 2021

In this roadmap article, we have focused on the most recent advances in terahertz (THz) imaging with particular attention paid to optimization and miniaturization of THz systems. Such systems entail enhanced functionality, reduced power consumption, increased convenience, thus being geared toward implementation real operational conditions. The article will touch upon advanced solid-state-based systems, including room temperature sensors arrays, as well their on-chip integration diffractive optical components. We cover current-state compact emission sources, both optolectronic electrically driven; emphasis is attributed beam-forming role imaging, holography spatial filtering, nano-imaging, computational imaging. A number techniques, such light-field homodyne spectroscopy, phase sensitive spectrometry, modulated continuous wave frequency combs, passive use artificial intelligence data processing optics development, be reviewed. This presents a structured snapshot current 2021 provides an opinion contemporary scientific technological challenges field, extrapolations possible further evolution

Language: Английский

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Enhancer redundancy in development and disease

Nature Reviews Genetics, Journal Year: 2021, Volume and Issue: 22(5), P. 324 - 336

Published: Jan. 12, 2021

Language: Английский

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Fluorescent Nanoparticles for Super-Resolution Imaging

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(15), P. 12495 - 12543

Published: June 27, 2022

Super-resolution imaging techniques that overcome the diffraction limit of light have gained wide popularity for visualizing cellular structures with nanometric resolution. Following pace hardware developments, availability new fluorescent probes superior properties is becoming ever more important. In this context, nanoparticles (NPs) attracted increasing attention as bright and photostable address many shortcomings traditional probes. The use NPs super-resolution a recent development provides focus current review. We give an overview different methods discuss their demands on NPs. then review in detail features, strengths, weaknesses each NP class to support these applications provide examples from utilization various biological systems. Moreover, we outlook future field opportunities material science multiplexed subcellular

Language: Английский

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