Neuronal activity modulates the incorporation of newly translated PSD-95 into a robust structure as revealed by STED and MINFLUX DOI Creative Commons
Clara‐Marie Gürth, Maria Augusta do Rego Barros Fernandes Lima, Victor Macarrón‐Palacios

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 20, 2023

Abstract The postsynaptic density component PSD-95 undergoes activity-dependent plasticity mechanisms that rely on protein synthesis and structural remodeling. How synaptic activity can influence these dynamics at the single synapse level remains unclear. Here we combine genome-editing, pulse-chase experiments, STED 3D MINFLUX nanoscopy hippocampal neuronal cultures to study integration of newly translated molecules sites their rearrangement within individual clusters near-molecular resolution. We show amount recruited synapses scales with size, modulates in a bidirectional manner, resulting less new following excitatory more inhibitory stimulation. Furthermore, has dispersed organization is largely robust long-lasting changes activity. Altogether, this work sheds light underlying level, adding previously inaccessible information.

Language: Английский

Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies DOI Creative Commons
Lei Zhang,

Dongwen Shen,

Jia-Zhen Yang

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Buffer-Free MINFLUX Imaging via Nanobody Points Accumulation DOI
Jing Wang, Zixing Gu, Longfang Yao

et al.

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Language: Английский

Citations

0
Bright photoactivatable probes based on triphenylethylene for Cu2+ detection in tap water and tea samples DOI
Zuo‐Qin Liang, Dongdong Song,

Zhuo-Cheng Li

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 434, P. 137439 - 137439

Published: Sept. 15, 2023

Language: Английский

Citations

10
Organic fluorescent probes for live-cell super-resolution imaging DOI Creative Commons
Xinxin Duan, Meng Zhang, Yuhui Zhang

et al.

Frontiers of Optoelectronics, Journal Year: 2023, Volume and Issue: 16(1)

Published: Nov. 10, 2023

Abstract The development of super-resolution technology has made it possible to investigate the ultrastructure intracellular organelles by fluorescence microscopy, which greatly facilitated life sciences and biomedicine. To realize imaging living cells, both advanced systems excellent fluorescent probes are required. Traditional have good availability, but that is not case for live-cell imaging. In this review, we first introduce principles various technologies their probe requirements, then summarize existing designs delivery strategies imaging, finally provide a brief conclusion overview future. Graphical

Language: Английский

Citations

10
Neuronal activity modulates the incorporation of newly translated PSD-95 into a robust structure as revealed by STED and MINFLUX DOI Creative Commons
Clara‐Marie Gürth, Maria Augusta do Rego Barros Fernandes Lima, Victor Macarrón‐Palacios

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 20, 2023

Abstract The postsynaptic density component PSD-95 undergoes activity-dependent plasticity mechanisms that rely on protein synthesis and structural remodeling. How synaptic activity can influence these dynamics at the single synapse level remains unclear. Here we combine genome-editing, pulse-chase experiments, STED 3D MINFLUX nanoscopy hippocampal neuronal cultures to study integration of newly translated molecules sites their rearrangement within individual clusters near-molecular resolution. We show amount recruited synapses scales with size, modulates in a bidirectional manner, resulting less new following excitatory more inhibitory stimulation. Furthermore, has dispersed organization is largely robust long-lasting changes activity. Altogether, this work sheds light underlying level, adding previously inaccessible information.

Language: Английский

Citations

9