Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 23, 2023

Abstract Micro-nano biorobots based on bacteria have demonstrated great potential for tumor diagnosis and treatment. The bacterial gene expression drug release should be spatiotemporally controlled to avoid in healthy tissues undesired toxicity. Herein, we describe an alternating magnetic field-manipulated tumor-homing developed by genetically modifying engineered Escherichia coli with Fe 3 O 4 @lipid nanocomposites. After accumulating orthotopic colon tumors female mice, the paramagnetic nanoparticles enable receive convert signals into heat, thereby initiating of lysis proteins under control a heat-sensitive promoter. then lyse, releasing its anti-CD47 nanobody cargo, that is pre-expressed within bacteria. robust immunogenicity lysate cooperates activate both innate adaptive immune responses, generating antitumor effects against not only but also distal mice. magnetically constant field-controlled motion enhanced targeting increased therapeutic efficacy. Thus, behavior can manipulated vivo field, achieving tumor-specific CD47 blockage precision immunotherapy.

Language: Английский

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Recent advances in nanoparticle-mediated antibacterial applications

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 482, P. 215075 - 215075

Published: Feb. 21, 2023

Language: Английский

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Magnetic Microrobots with Folate Targeting for Drug Delivery

Cyborg and Bionic Systems, Journal Year: 2023, Volume and Issue: 4

Published: Jan. 1, 2023

Untethered microrobots can be used for cargo delivery (e.g., drug molecules, stem cells, and genes) targeting designated areas. However, it is not enough to just reach the lesion site, as some drugs only play best therapeutic effect within cells. To this end, folic acid (FA) was introduced into in work a key mediate endocytosis of The here were fabricated with biodegradable gelatin methacryloyl (GelMA) modified magnetic metal–organic framework (MOF). porous structure MOF hydrogel network polymerized GelMA loading FA anticancer doxorubicin (DOX) respectively. Utilizing property MOF, these gather around site navigation fields. combination effects substantially improve efficiency microrobots. result shows that cancer cells inhibition rate up 93%, while ones without 78%. introduction useful method transportation ability microrobots, providing meaningful reference further research.

Language: Английский

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Swarming magnetic nanorobots bio-interfaced by heparinoid-polymer brushes for in vivo safe synergistic thrombolysis

Science Advances, Journal Year: 2023, Volume and Issue: 9(48)

Published: Nov. 29, 2023

Biocompatible swarming magnetic nanorobots that work in blood vessels for safe and efficient targeted thrombolytic therapy vivo are demonstrated. This is achieved by using beads elaborately grafted with heparinoid-polymer brushes (HPBs) upon the application of an alternating field B ( t ). Because dense surface charges bestowed HPBs, demonstrate reversible agglomeration-free reconfigurations, low hemolysis, anti-bioadhesion, self-anticoagulation high-ionic-strength environments. They confirmed vitro to perform synergistic thrombolysis efficiently “motile-targeting” drug delivery mechanical destruction. Moreover, completion removal ), disassemble into dispersed particles blood, allowing them safely participate circulation be phagocytized immune cells without apparent organ damage or inflammatory lesion. provides a rational multifaceted HPB biointerfacing design strategy biomedical general motile platform deliver drugs therapies.

Language: Английский

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Engineered Living Materials for Advanced Diseases Therapy

Advanced Materials, Journal Year: 2023, Volume and Issue: unknown

Published: July 12, 2023

Abstract Natural living materials serving as biotherapeutics exhibit great potential for treating various diseases owing to their immunoactivity, tissue targeting, and other biological activities. In this review, the recent developments in engineered materials, including mammalian cells, bacteria, viruses, fungi, microalgae, plants, active derivatives that are used summarized. Further, future perspectives challenges of such material‐based discussed provide considerations advances biomedical applications.

Language: Английский

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Autonomous metal-organic framework nanorobots for active mitochondria-targeted cancer therapy

Science Advances, Journal Year: 2023, Volume and Issue: 9(23)

Published: June 9, 2023

Nanorobotic manipulation to access subcellular organelles remains unmet due the challenge in achieving intracellular controlled propulsion. Intracellular organelles, such as mitochondria, are an emerging therapeutic target with selective targeting and curative efficacy. We report autonomous nanorobot capable of active mitochondria-targeted drug delivery, prepared by facilely encapsulating mitochondriotropic doxorubicin-triphenylphosphonium (DOX-TPP) inside zeolitic imidazolate framework-67 (ZIF-67) nanoparticles. The catalytic ZIF-67 body can decompose bioavailable hydrogen peroxide overexpressed tumor cells generate effective movement presence TPP cation. This nanorobot-enhanced targeted delivery induces mitochondria-mediated apoptosis mitochondrial dysregulation improve vitro anticancer effect suppression cancer cell metastasis, further verified vivo evaluations subcutaneous model orthotopic breast model. unlocks a fresh field operation organelle access, thereby introducing next generation robotic medical devices organelle-level resolution for precision therapy.

Language: Английский

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Ultrasound trapping and navigation of microrobots in the mouse brain vasculature

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 21, 2023

The intricate and delicate anatomy of the brain poses significant challenges for treatment cerebrovascular neurodegenerative diseases. Thus, precise local drug delivery in hard-to-reach regions remains an urgent medical need. Microrobots offer potential solutions; however, their functionality restricted by limited imaging capabilities complications within blood vessels, such as high flows, osmotic pressures, cellular responses. Here, we introduce ultrasound-activated microrobots vivo navigation vasculature. Our consist lipid-shelled microbubbles that autonomously aggregate propel under ultrasound irradiation. We investigate capacities vitro microfluidic-based vasculatures vessels a living mouse brain. These self-assemble execute upstream motion vasculature, achieving velocities up to 1.5 µm/s moving against flows ~10 mm/s. This work represents substantial advance towards therapeutic application complex

Language: Английский

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Drug-Free Antimicrobial Nanomotor for Precise Treatment of Multidrug-Resistant Bacterial Infections

Nano Letters, Journal Year: 2023, Volume and Issue: 23(9), P. 3929 - 3938

Published: April 27, 2023

Manufacturing heteronanostructures with specific physicochemical characteristics and tightly controllable designs is very appealing. Herein, we reported NIR-II light-driven dual plasmonic (AuNR-SiO2-Cu7S4) antimicrobial nanomotors an intended Janus configuration through the overgrowth of copper-rich Cu7S4 nanocrystals at only one high-curvature site Au nanorods (Au NRs). These were applied for photoacoustic imaging (PAI)-guided synergistic photothermal photocatalytic treatment bacterial infections. Both performance activity are dramatically improved owing to strong plasmon coupling between NRs component enhanced energy transfer. The motion behavior promotes transdermal penetration enhances matter–bacteria interaction. More importantly, directional navigation could be synchronously driven by light. marriage active antibacterial resulted in expected good effects abscess infection mouse model.

Language: Английский

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Multifunctional Integrated Nanozymes Facilitate Spinal Cord Regeneration by Remodeling the Extrinsic Neural Environment

Advanced Science, Journal Year: 2023, Volume and Issue: 10(7)

Published: Jan. 16, 2023

High levels of reactive oxygen species (ROS) and inflammation create a complicated extrinsic neural environment that dominates the initial post-injury period after spinal cord injury (SCI). The compensatory pathways between ROS limited efficacy modulating above single treatment regimen SCI. Here, novel "nanoflower" Mn

Language: Английский

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Multimodal‐Driven Magnetic Microrobots with Enhanced Bactericidal Activity for Biofilm Eradication and Removal from Titanium Mesh

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(23)

Published: March 30, 2023

Modern micro/nanorobots can perform multiple tasks for biomedical and environmental applications. Particularly, magnetic microrobots be completely controlled by a rotating field their motion powered without the use of toxic fuels, which makes them most promising application. Moreover, they are able to form swarms, allowing specific at larger scale than single microrobot. In this work, developed composed halloysite nanotubes as backbone iron oxide (Fe3 O4 ) nanoparticles material propulsion covered these with polyethylenimine load ampicillin prevent from disassembling. These exhibit multimodal robots well in swarms. addition, transform tumbling spinning vice-versa, when swarm mode change vortex ribbon back again. Finally, is used penetrate disrupt extracellular matrix Staphylococcus aureus biofilm colonized on titanium mesh bone restoration, improves effect antibiotic's activity. Such removal medical implants could reduce implant rejection improve patients' well-being.

Language: Английский

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