Outer membrane lipoproteins: late to the party, but the center of attention DOI Creative Commons
Kerrie L. May, Marcin Grabowicz

Journal of Bacteriology, Journal Year: 2024, Volume and Issue: 207(1)

Published: Dec. 13, 2024

An outer membrane (OM) is the hallmark feature that often used to distinguish "Gram-negative" bacteria. Our understanding of how OM built rests largely on studies Escherichia coli. In organism-and seemingly in all species Proteobacterial phyla-the essential pathways assemble each rely one or more lipoproteins have been trafficked OM. Hence, lipoprotein trafficking pathway appeared be foundational for ability these bacteria build their However, such a notion now appears misguided. New phylogenetic analyses show us was likely very last assembly systems evolved. The emergence must powerful innovation ancestors Proteobacteria, given it assumed central place biogenesis. this minireview, we broadly discuss biosynthesis and ponder why newest system (lipoprotein trafficking) has become so key building We examine diversity among systems, noting uniting commonalities highlighting differences. Current novel antibiotic development targeted against small subset cause severe human diseases; several inhibitors recently reported may new antibiotics. Understanding yield selective antibiotics preferentially kill important pathogens while sparing normal healthy flora.

Language: Английский

Ancient origin and constrained evolution of the division and cell wall gene cluster in Bacteria DOI
Daniela Megrian, Najwa Taïb, Alexander L. Jaffe

et al.

Nature Microbiology, Journal Year: 2022, Volume and Issue: 7(12), P. 2114 - 2127

Published: Nov. 21, 2022

Language: Английский

Citations

39
Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacterium Deinococcus radiodurans DOI Creative Commons
Andriko von Kügelgen, Sofie van Dorst, Keitaro Yamashita

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(16)

Published: April 12, 2023

is an atypical diderm bacterium with a remarkable ability to tolerate various environmental stresses, due in part its complex cell envelope encapsulated within hyperstable surface layer (S-layer). Despite decades of research on this envelope, atomic structural details the S-layer have remained obscure. In study, we report electron cryomicroscopy structure

Language: Английский

Citations

24
Valid publication of names of two domains and seven kingdoms of prokaryotes DOI
Markus Göker, Aharon Oren

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(1)

Published: Jan. 22, 2024

The International Code of Nomenclature Prokaryotes (ICNP) now includes the categories domain and kingdom. For purpose valid publication their names under ICNP, we consider here two known domains, ‘ Bacteria ’ Archaea ’, as well a number taxa suitable for rank kingdom, based on previous phylogenetic taxonomic studies. It is proposed to subdivide into kingdoms Bacillati , Fusobacteriati Pseudomonadati Thermotogati . This arrangement reflects contemporary hypotheses proposals cell wall structure, including ‘diderms’ vs. ‘monoderms’, Gracilicutes Firmicutes Negibacteria Unibacteria Hydrobacteria Terrabacteria Hydrobacterida Terrabacterida ’. include Methanobacteriati Nanobdellati Thermoproteati reflecting division Euryarchaeota ‘DPANN superphylum’ ‘TACK superphylum’.

Language: Английский

Citations

14
The multiplicity of thioredoxin systems meets the specific lifestyles of Clostridia DOI Creative Commons
Cyril Anjou, Aurélie Lotoux, Anna Zhukova

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(2), P. e1012001 - e1012001

Published: Feb. 8, 2024

Cells are unceasingly confronted by oxidative stresses that oxidize proteins on their cysteines. The thioredoxin (Trx) system, which is a ubiquitous system for thiol and protein repair, composed of (TrxA) reductase (TrxB). TrxAs reduce disulfide bonds oxidized then usually recycled single pleiotropic NAD(P)H-dependent TrxB (NTR). In this work, we first analyzed the composition Trx systems across Bacteria. Most bacteria have only one NTR, but organisms in some Phyla several TrxBs. Firmicutes, multiple TrxBs observed Clostridia, with another peculiarity being existence ferredoxin-dependent We used Clostridioides difficile , pathogenic sporulating anaerobic as model to investigate biological relevance multiplicity. Three three present 630Δ erm strain. showed two involved response infection-related stresses, allowing survival vegetative cells exposed oxygen, inflammation-related molecules bile salts. A fourth copy strains also contributes stress-response arsenal. One conserved was found be both spores under dual transcriptional control cell sporulation sigma factors. This spore hypochlorite ensure proper germination presence oxygen. Finally, third through recycling glycine-reductase, Stickland pathway enzyme allows consumption glycine sporulation. Altogether, produced various regulatory signals respond different networks. multiplicity diversity most likely meet specific needs Clostridia adaptation strong stress exposure, pathways.

Language: Английский

Citations

10
Terrabacteria: redefining bacterial envelope diversity, biogenesis and evolution DOI

Basile Beaud Benyahia,

Najwa Taïb, Christophe Beloin

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 23(1), P. 41 - 56

Published: Aug. 28, 2024

Language: Английский

Citations

8
Modern microbiology: Embracing complexity through integration across scales DOI Creative Commons
A. Murat Eren, Jillian F. Banfield

Cell, Journal Year: 2024, Volume and Issue: 187(19), P. 5151 - 5170

Published: Sept. 1, 2024

Language: Английский

Citations

6
A multidomain connector links the outer membrane and cell wall in phylogenetically deep-branching bacteria DOI Creative Commons
Andriko von Kügelgen, Sofie van Dorst, Vikram Alva

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(33)

Published: Aug. 9, 2022

Deinococcus radiodurans is a phylogenetically deep-branching extremophilic bacterium that remarkably tolerant to numerous environmental stresses, including large doses of ultraviolet (UV) radiation and extreme temperatures. It can even survive in outer space for several years. This endurance D. has been partly ascribed its atypical cell envelope comprising an inner membrane, periplasmic with thick peptidoglycan (PG) layer, membrane (OM) covered by surface layer (S-layer). Despite intense research, molecular principles governing organization OM stabilization are unclear related bacteria. Here, we report electron cryomicroscopy (cryo-EM) structure the abundant protein SlpA, showing how C-terminal segment forms homotrimers 30-stranded β-barrels OM, whereas N-terminal long, homotrimeric coiled coils linking PG via S-layer homology (SLH) domains. Furthermore, using prediction sequence-based bioinformatic analysis, show SlpA-like putative OM–PG connector proteins widespread Gram-negative Finally, combining our atomic structures fluorescence microscopy envelopes wild-type mutant bacterial strains, model . Our results will have important implications understanding hyperstability bacteria evolutionary transition between Gram-positive

Language: Английский

Citations

27
Teasing apart the evolution of lipoprotein trafficking in gram-negative bacteria reveals a bifunctional LolA DOI Creative Commons
Hannah C. Smith, Kerrie L. May, Marcin Grabowicz

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(6)

Published: Jan. 30, 2023

The outer membrane (OM) is the defining feature of gram-negative bacteria and an essential organelle. Accordingly, OM assembly pathways their protein components are conserved throughout all species. Lipoprotein trafficking lies at heart since it supplies several different biogenesis machines with lipoproteins. Escherichia coli Lol pathway relies on inner LolCDE transporter that transfers newly made lipoproteins to chaperone LolA, which rapidly traffics across periplasm LolB for insertion into OM. Strikingly, many species (like Caulobacter vibrioides ) do not produce LolB, yet still trafficked How final step occurs in these organisms has remained a long-standing mystery. We demonstrate LolA from C. can complement deletion both E. , revealing this possesses activities. Moreover, we define region responsible its bifunctionality. This knowledge enabled us convert similarly bifunctional protein, capable propose eliminates need LolB. Our findings provide explanation why some have retained completely lack dedicated protein.

Language: Английский

Citations

11
Punctuated and continuous structural diversity of S-layers across the prokaryotic tree of life DOI Creative Commons
Ed Johnston, Buse Isbilir, Vikram Alva

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract Surface layers (S-layers) are two-dimensional (2D) crystalline lattices that frequently coat prokaryotic cells, playing a crucial role in protection, maintaining cellular integrity, and mediating environmental interactions. However, the molecular landscape of these abundant proteins has remained underexplored due to lack structural data. By employing AlphaFold2multimer together with planar symmetry constraints workflow validated by electron cryomicroscopy structure determination, we have elucidated lattice structures over 150 S-layers from diverse archaea bacteria. Our findings unveil multifaceted evolutionary for S-layer proteins, highlighting key differences evolution bacterial archaeal S-layers. study allows us discover underlying patterns structure, organisa-tion, cell anchoring mechanisms across tree life, deepening our understanding intricately complex microbial surfaces, which appear evolved proteinaceous independently on multiple occasions. This work will open avenues rational manipulation interactions multicellular microbiomes, as well innovative 2D biomaterial design.

Language: Английский

Citations

4
LD-transpeptidase-mediated cell envelope remodeling enables developmental transitions and survival in Coxiella burnetii and Legionella pneumophila DOI Creative Commons
Dipak Kathayat, Yujia Huang,

J Denis

et al.

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

ABSTRACT Coxiella burnetii and Legionella pneumophila are two phylogenetically related bacterial pathogens that exhibit extreme intrinsic resistance when they enter into a dormancy-like state. This enables both to survive extended periods in growth-limited environments. Survival is dependent upon their ability undergo developmental transitions phenotypically distinct variants, one specialized for intracellular replication another prolonged survival the environment host. We currently lack an understanding of mechanisms mediate these transitions. Here, we performed peptidoglycan (PG) glycoproteome analysis showed significant enrichment PG structures catalyzed by LD-transpeptidases (LDTs) variants C. L. . supported upregulation LDTs, resulting susceptibility carbapenem antibiotics. Furthermore, deletion most upregulated LDT, lpg 1386, significantly changes architecture, survival, Significantly regulated RpoS, stationary-phase sigma factor, LDT-dependent remodeling differentially activated host growth compared axenic culture. In addition, β-barrel tethering, newly discovered mechanism LDT-mediated cell envelope stabilization, seems not be specific variants. Interestingly, outer membrane (OM) long-chain fatty acid transporter (Lpg1810) tethered Collectively, findings show major determinant Understanding this might inform new therapeutic approaches treating chronic infections caused pathogens, as well suggest methods decontaminate environmental reservoirs during outbreaks. IMPORTANCE cause Q Fever Legionnaire’s disease humans, respectively. There effective treatments fatal particularly Fever. These bacteria long term nutrient-limited environments differentiating Our study revealed group enzymes, play prominent role phenotypic differentiations bacteria. LDT-targeting carbapenems against thus demanding exploration pathogens. report tethering unique OM could indicate novel function is, uptake nutrient substrates. Homologs widely present Methylobacteriaceae family known water systems like , suggesting potentially conserved

Language: Английский

Citations

0