Electro-Biofabrication. Coupling Electrochemical and Biomolecular Methods to Create Functional Bio-Based Hydrogels

Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(6), P. 2409 - 2432

Published: May 8, 2023

Twenty years ago, this journal published a review entitled "Biofabrication with Chitosan" based on the observations that (i) chitosan could be electrodeposited using low voltage electrical inputs (typically less than 5 V) and (ii) enzyme tyrosinase used to graft proteins (via accessible tyrosine residues) chitosan. Here, we provide progress report coupling of electronic advanced biological methods for fabrication biopolymer-based hydrogel films. In many cases, initial chitosan's electrodeposition have been extended generalized: mechanisms established various other polymers (proteins polysaccharides), has shown allow precise control hydrogel's emergent microstructure. addition, use biotechnological confer function from conjugation protein engineering create genetically fused assembly tags (short sequences amino acid facilitate attachment function-conferring films alternative enzymes (e.g., transglutaminase), metal chelation, electrochemically induced oxidative mechanisms. Over these 20 years, contributions numerous groups also identified exciting opportunities. First, electrochemistry provides unique capabilities impose chemical cues can induce while controlling Second, it is clear detailed biopolymer self-assembly (i.e., gel formation) are far more complex anticipated, rich opportunity both fundamental inquiry creation high performance sustainable material systems. Third, mild conditions cells co-deposited living materials. Finally, applications expanded biosensing lab-on-a-chip systems bioelectronic medical We suggest electro-biofabrication poised emerge as an enabling additive manufacturing method especially suited life science bridge communication between our technological worlds.

Language: Английский

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Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 21, 2023

Abstract Microelectronic devices can directly communicate with biology, as electronic information be transmitted via redox reactions within biological systems. By engineering biology’s native networks, we enable interrogation and control of systems at several hierarchical levels: proteins, cells, cell consortia. First, electro-biofabrication facilitates on-device component assembly. Then, electrode-actuated data transmission redox-linked synthetic biology allows programming enzyme activity closed-loop electrogenetic cellular function. Specifically, horseradish peroxidase is assembled onto interdigitated electrodes where electrode-generated hydrogen peroxide controls its activity. E. coli ’s stress response regulon, oxyRS , rewired to algorithm-based feedback gene expression, including an eCRISPR module that switches cell-cell quorum sensing communication from one autoinducer another—creating electronically controlled ‘bilingual’ cell. these disparate redox-guided are wirelessly connected, enabling real-time user-based control. We suggest methodologies will help us better understand develop sophisticated for biology.

Language: Английский

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At the crossroads of biology and electronics

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 91, P. 103249 - 103249

Published: Jan. 9, 2025

Language: Английский

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Skin‐Integrated Electrogenetic Regulation of Vasculature for Accelerated Wound Healing

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Neo‐vascularization plays a key role in achieving long‐term viability of engineered cells contained medical implants used precision medicine. Moreover, strategies to promote neo‐vascularization around may also be useful the healing deep wounds. In this context, biocompatible, electroconducti v e b o rophene–poly(ε‐capro l ac t one) (PCL) 3D platform is developed, which called VOLT, support designer with direct‐current (DC) voltage‐controlled gene circuit that drives secretion vascular endothelial growth factor A (VEGFA). The VOLT consists 3D‐printed borophene‐PCL honeycomb‐shaped matrix decorated nanofibers by electrospinning. honeycomb structure provides mechanical stability, while facilitate adhesion, migration, and proliferation cells. incorporate DC‐powered reactive oxygen species (ROS)‐sensing wired an synthetic promoter triggers VEGFA vascularization adjacent extracellular matrix. Cells enclosed matrix, termed system, can simply triggered using off‐the‐shelf AA batteries, utilizing established ability brief DC bias generate non‐cytotoxic levels ROS. For proof‐of‐concept, subcutaneous wound‐healing model rats chosen. Electrostimulation implant (5 V, 20 s per day) induced VEGFA, significantly accelerated neovascularization granulation tissue formation, resulting faster wound closure compared non‐stimulated controls. Complete re‐epithelialization dermal regeneration are observed within 15 days application.

Language: Английский

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3D Printed Spectroelectrochemical Platform for Redox‐Based Bioelectronics

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Redox provides unique opportunities for interconverting molecular/biological information into electronic signals. Here, the fabrication of a 3D‐printed multiwell device that can be interfaced existing laboratory instruments (e.g., well‐plate readers and microscopes) to enable advanced redox‐based spectral electrochemical capabilities is reported. In first application, mediated probing used as soft sensing method biomanufacturing: it shown signal metrics discern intact mAbs from partially reduced mAb variants (fragmentation), these near‐real‐time electrical measurements correlate off‐line chemical analysis. second operando spectroelectrochemical are characterize redox‐active catechol‐based hydrogel film: electron transfer into/from film correlates molecular switching film's redox state with absorbance increasing upon oxidation fluorescence reduction. final example, synthetic biofilm containing redox‐responsive E. coli electro‐assembled: gene expression induced under reducing conditions (via reductive H 2 O generation) or oxidative phenolic redox‐signaling molecule). Overall, this work demonstrates 3D printing allows bespoke devices accelerate understanding phenomena in biology detection/characterization activities technology.

Language: Английский

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