Non-aqueous electrowetting liquid lens with centimeter-level large aperture based on dielectric failure suppression principle

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: March 12, 2025

Abstract Liquid lens offers a novel approach to achieving large depth of field, wide viewing angle, high speed, and high-quality imaging in zoom optical systems. However, the aperture reliability limit lens’s performance various applications. The liquid material is crucial for large-aperture lens. To solve dielectric failure problem associated with aperture, we first reveal mechanism based on transport properties electrolyte solutions impact electrochemical reaction rates from physical chemistry so as propose theoretical method suppress fundamentally. Based this theory, develop series non-aqueous organic high-voltage failure. Next, identify optimal formulation comprehensive fabricate centimeter-level electrowetting This features an power variation range −11.98 m −1 12.93 , clear function, which can enlarge field view adjustment holographic reconstructions while maintaining excellent edge clarity reconstructed images. proposed effectively suppresses under voltage, demonstrates performance, holds exciting potential applications 3D display, precision measurement, biomedical observation, more.

Language: Английский

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A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Recent advancements in genetically encoded calcium indicators, particularly those based on green fluorescent proteins, have optimized their performance for monitoring neuronal activities a variety of model organisms. However, progress developing red-shifted GECIs, despite advantages over has been slower, resulting fewer options end-users. In this study, we explored topological inversion and soma-targeting strategies, which are complementary to conventional mutagenesis, re-engineer red indicator, FRCaMP, enhanced vivo performance. The sensors, FRCaMPi soma-targeted (SomaFRCaMPi), exhibit up 2-fold higher dynamic range peak ΔF/F 0 per single AP compared widely used jRGECO1a neurons culture . Compared FRCaMPi, SomaFRCaMPi reduces erroneous correlation activity the brains mice zebrafish by two- four-fold due diminished neuropil contamination without compromising signal-to-noise ratio. Under wide-field imaging primary somatosensory visual cortex with high labeling density (80-90%), exhibits 40% SNR decreased artifactual across neurons. Altogether, improves accuracy scale at single-neuron resolution densely labeled brain tissues 2-3-fold automated segmentation, 50% fraction responsive cells, jRGECO1a. Our findings highlight potential SomaFRCaMPi, comparable most sensitive GCaMP, precise spatial recording populations using popular modalities organisms such as mice.

Language: Английский

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Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский

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液体透镜在显微成像系统中的应用

Laser & Optoelectronics Progress, Journal Year: 2025, Volume and Issue: 62(8), P. 0800002 - 0800002

Published: Jan. 1, 2025

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Fast-zoom and high-resolution sparse compound-eye camera based on dual-end collaborative optimization

Opto-Electronic Advances, Journal Year: 2025, Volume and Issue: 0(0), P. 240285 - 240285

Published: Jan. 1, 2025

Language: Английский

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A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(4), P. e3003048 - e3003048

Published: April 29, 2025

Recent advancements in genetically encoded calcium indicators, particularly those based on green fluorescent proteins, have optimized their performance for monitoring neuronal activities a variety of model organisms. However, progress developing red-shifted GECIs, despite advantages over has been slower, resulting fewer options end users. In this study, we explored topological inversion and soma-targeting strategies, which are complementary to conventional mutagenesis, re-engineer red indicator, FRCaMP, enhanced vivo performance. The sensors, FRCaMPi soma-targeted (SomaFRCaMPi), exhibit up 2-fold higher dynamic range peak Δ F / 0 per single AP compared widely used jRGECO1a neurons both culture vivo. Compared FRCaMPi, SomaFRCaMPi reduces erroneous correlation activity the brains mice zebrafish by two- 4-fold due diminished neuropil contamination without compromising signal-to-noise ratio. Under wide-field imaging primary somatosensory visual cortices with high labeling density (80–90%), exhibits 40% SNR decreased artifactual across neurons. Altogether, improves accuracy scale at single-neuron resolution densely labeled brain tissues 2–3-fold automated segmentation, 50% fraction responsive cells, jRGECO1a. Our findings highlight potential SomaFRCaMPi, comparable most sensitive GCaMP, precise spatial recording populations using popular modalities organisms such as mice.

Language: Английский

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Off-axis reflective microscope objective with a centimeter scale field of view and micron resolution

Optics Express, Journal Year: 2024, Volume and Issue: 32(16), P. 27497 - 27497

Published: July 5, 2024

Microscope objectives with wide field-of-view (FOV) and high resolution are urgently needed for the frontier research in life sciences. However, traditional transmission microscope typically have a narrow FOV severe chromatic aberration. A new off-axis reflective objective centimeter scale micron is proposed this paper. This objective, its simple structure, can operate over wavelength range. design method optical system presented, which eliminate obstruction of rays control intermediate image plane. Using method, we novel four-mirror 10 mm × 1.5 numerical aperture 0.33.

Language: Английский

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An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

The mesoscope has emerged as a powerful imaging tool in biomedical research, yet its high cost and low resolution have limited broader application. Here, we introduce the Omni-Mesoscope, cost-effective high-spatial-temporal, multimodal, multiplex mesoscopic platform built from cost-efficient off-the-shelf components. This system uniquely merges capabilities of quantitative phase microscopy to capture live-cell dynamics over large cell population with highly multiplexed fluorescence for comprehensive molecular characterization. integration facilitates simultaneous tracking morphodynamics across thousands cells, alongside high-content analysis at single-cell level. Furthermore, Omni-Mesoscope offers mesoscale field view approximately 5 mm

Language: Английский

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Three-dimensional random-access confocal microscopy with 3D remote focusing system

Communications Engineering, Journal Year: 2024, Volume and Issue: 3(1)

Published: Nov. 11, 2024

Understanding biological activities in cells or deep tissues requires high-speed three-dimensional (3D) imaging. Substantial progress has been made with the emergence of 3D random-access microscopy. However, current solutions for fast imaging remain complex and costly. Herein we propose a simple, cost-effective, confocal microscopy remote focusing system. Our system shows isotropic response times across x, y, z axes, 34-fold improvement axial time over traditional piezo stages. We demonstrate its volumetric performance fluorescent particles live cells. Furthermore, validate capability this by continuously monitoring signals three different planes, showing refresh rate 500 Hz on two positions 3D. The simplicity, versatility, affordability our promise widespread applications research industry.

Language: Английский

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An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization

Science Advances, Journal Year: 2024, Volume and Issue: 10(42)

Published: Oct. 16, 2024

The mesoscope has emerged as a powerful imaging tool in biomedical research, yet its high cost and low resolution have limited broader application. Here, we introduce the Omni-Mesoscope, high–spatial-temporal multimodal mesoscopic platform built from cost-efficient off-the-shelf components. This system uniquely merges capabilities of label-free quantitative phase microscopy to capture live-cell morphodynamics across thousands cells with highly multiplexed fluorescence for comprehensive molecular characterization. Omni-Mesoscope offers mesoscale field view ~5 square millimeters spatial down 700 nanometers, enabling detailed subcellular features. We demonstrate capability delineating characteristics underlying rare morphodynamic cellular phenomena, including cancer cell responses chemotherapy emergence polyploidy drug-resistant cells. also integrate expansion technique enhance three-dimensional volumetric super-resolution thicker tissues, opening avenues biological exploration at unprecedented scales resolutions.

Language: Английский

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