An all-reflective freeform microscope objective for ultra-broadband microscopy DOI Creative Commons
Aaron Bauer, Jannick P. Rolland,

Stephan Clark

et al.

Optics Express, Journal Year: 2024, Volume and Issue: 32(27), P. 47893 - 47893

Published: Dec. 5, 2024

Conventional refractive microscope objective lenses have limited applicability to a range of imaging modalities due the dispersive nature their optical elements. Designing conventional that provides well-corrected over broad spectral can be challenging, if not impossible. In contrast, reflective optics are inherently achromatic, so system composed entirely elements is free from chromatic aberrations and, as result, image an ultra-wide with perfect color correction. This study explores design space unobscured high numerical aperture, all-reflective objectives. particular, using freeform we obviate need for center obscuration, rendering objective's modulation transfer function comparable lens systems similar aperture. We detail process objective, determining specifications optimization and sensitivity analysis. The outcome 0.65 aperture diffraction-limited compatible geometric constraints commercial systems.

Language: Английский

Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy for rapid, label-free biological imaging DOI
Jessica Z. Dixon, Wei‐Wen Chen, Haoyu Xu

et al.

Review of Scientific Instruments, Journal Year: 2025, Volume and Issue: 96(4)

Published: April 1, 2025

Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy is a label-free imaging approach that provides detailed chemical information at high spatial resolution in sample through nonlinear, excitation of molecular vibrations and detection spectra. While its utility for biological has been demonstrated, many aspects this technique must mature before it can be widely adopted. One the areas required improvement speed—most BCARS implementations involve rastering, which limits speed. Beam scanning provide faster but presents some unique challenges. Here, we describe beam-scanning system improves twofold speed by fivefold over previous implementation. These enhancements were enabled an supercontinuum power use sCMOS camera data transfer rate low read noise. Implementation correction significant pixel-to-pixel background photon response nonuniformity. We report on method implemented calibrating correcting differences

Language: Английский

Citations

0
An all-reflective freeform microscope objective for ultra-broadband microscopy DOI Creative Commons
Aaron Bauer, Jannick P. Rolland,

Stephan Clark

et al.

Optics Express, Journal Year: 2024, Volume and Issue: 32(27), P. 47893 - 47893

Published: Dec. 5, 2024

Conventional refractive microscope objective lenses have limited applicability to a range of imaging modalities due the dispersive nature their optical elements. Designing conventional that provides well-corrected over broad spectral can be challenging, if not impossible. In contrast, reflective optics are inherently achromatic, so system composed entirely elements is free from chromatic aberrations and, as result, image an ultra-wide with perfect color correction. This study explores design space unobscured high numerical aperture, all-reflective objectives. particular, using freeform we obviate need for center obscuration, rendering objective's modulation transfer function comparable lens systems similar aperture. We detail process objective, determining specifications optimization and sensitivity analysis. The outcome 0.65 aperture diffraction-limited compatible geometric constraints commercial systems.

Language: Английский

Citations

2