Revolutionizing the female reproductive system research using microfluidic chip platform DOI Creative Commons
Jinfeng Yan, Tong Wu, Jinjin Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Dec. 19, 2023

Abstract Comprehensively understanding the female reproductive system is crucial for safeguarding fertility and preventing diseases concerning women's health. With capacity to simulate intricate physio- patho-conditions, provide diagnostic platforms, microfluidic chips have fundamentally transformed knowledge management of health, which will ultimately promote development more effective assisted technologies, treatments, drug screening approaches. This review elucidates diverse systems in mimicking ovary, fallopian tube, uterus, placenta cervix, we delve into culture follicles oocytes, gametes’ manipulation, cryopreservation, permeability especially. We investigate role microfluidics endometriosis hysteromyoma, explore their applications ovarian cancer, endometrial cancer cervical cancer. At last, current status technology integrated devices are introduced briefly. Through delineating multifarious advantages challenges technology, chart a definitive course future research woman health field. As continues evolve advance, it holds great promise revolutionizing diagnosis treatment issues, thus propelling us where can optimize overall wellbeing women everywhere. Graphical

Language: Английский

Multiresponsive Microcapsules for Prevention of Intrauterine Adhesion DOI
Jing Wang, Qiong Chen, Yueyue Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Intrauterine adhesion (IUA) has a significant negative impact on women's reproductive health. One of the development trends biomaterials for prevention IUA is improving stability and multifaceted functions. Here, we propose multiresponsive microcapsule (A/G-Fe3O4-Se) with an alginate (ALG) gelatin methacryloyl (GelMA) dual-network hydrogel shell loaded magnetic nanoparticles (Fe3O4-Se) ultrasound-responsive decafluoropentane core using microfluidic technique. The microcapsules inherited biofriendly advantages ALG GelMA. encapsulated magneto-responsive Fe3O4-Se made flexibly change distribution to adapt irregular shape uterus better exert therapeutic effect. Besides, A/G-Fe3O4-Se demonstrated antioxidant, antibacterial, pro-healing properties in vitro. Moreover, rat models, also observed reduction oxidative stress, endometrial regeneration, improved receptivity pregnancy rates after treatment microcapsules. Consequently, can be used as promising strategy damaged endometrium well IUA.

Language: Английский

Citations

0
Evolving from organoid to assembloid with enhanced cellular interactions DOI
Jiarui Liu, Yitong Shi, Xinkun Shen

et al.

Cell organoid (Print), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Construction organoid model of ovarian endometriosis and the function of estrogen and progesterone in the model DOI Creative Commons
Ruiqi Zhang, Yue Yang, Ruyue Li

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 24, 2025

Abstract Endometriosis is a refractory estrogen-dependent gynecological disease in which ovarian endometriosis(OE) the most common, and main cell components are endometrial epithelial cells stromal cells. However, constructing ectopic models basic studies still challenging. In this study, we explored feasibility influencing factors of validating eutopic organoid OE as in-vitro models. Eutopic tissues patients were selected to establish organoids. Morphologically, organoids showed three-dimensional glandular structure with vacuoles or cystic irregularities, histological features endometriosis well preserved. Immunofluorescence positive expression markers estrogen/progesterone receptors. Genetic identification revealed 100% match between tissue, indicating common origin. The effects estrogen progesterone on proliferation secretion differed change concentration. successful construction provides new vitro model for drug intervention mechanism study endometriosis.

Language: Английский

Citations

0
Chemerin's Role in Endometrial Dysfunction: Insights From Transcriptomic Analysis DOI Creative Commons
Ming Yu, Yichun Wang, Jie Cai

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(6)

Published: March 1, 2025

ABSTRACT Endometrium, the lining of uterus, changes dynamically in response to fluctuations ovarian hormones. The proper endocrine environment regulates endometrial functions: menstruation and supporting pregnancy. Obesity is closely related dysfunction, which seriously affects women's health fertility, but pathological mechanism unknown. Chemerin an adipokine involved multiple biological events such as immunity metabolism by acting on its functional receptors. This study aimed characterise effects chemerin human epithelial cells RNA‐Seq. 12Z were utilised model because immunoblot results showed that they expressed markers, markers receptors for chemerin. Principal component analysis (PCA) treatment significantly altered transcriptome. Differential Expression Analysis found 388 significant differentially genes (DEG) group compared with controls. Gene Set Enrichment (GSEA) inhibited lipid induced epithelial‐mesenchymal transition (EMT)‐like process cellular senescence. More importantly, GSEA immunoblots restrained STAT3 signalling pathway, required receptivity establishment. Collectively, these findings provide new evidence over‐produced underlying dysfunctions obesity.

Language: Английский

Citations

0
The human endometrium: atlases, models, and prospects DOI
Frederick C.K. Wong,

C Kim,

Luz García‐Alonso

et al.

Current Opinion in Genetics & Development, Journal Year: 2025, Volume and Issue: 92, P. 102341 - 102341

Published: March 29, 2025

Language: Английский

Citations

0
Ovarian Endometriosis Accelerates Premature Ovarian Failure and Contributes to Osteoporosis and Cognitive Decline in Aging Mice DOI Open Access
Lei Ge, Yali Yang, Tianxia Xiao

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3313 - 3313

Published: April 2, 2025

Ovarian endometriosis (OEM) is a chronic inflammatory condition that impairs ovarian function. While its effects on reserve are well established, the long-term consequences of OEM dysfunction, premature failure (POF), and systemic health, particularly in context accelerated aging, remain insufficiently studied. In this study, we employed an mouse model bulk RNA sequencing to investigate underlying mechanisms. Our results show accelerates primordial follicle depletion upregulates mTOR signaling pathway gene expression, along with mechanical stress paracrine via Hippo Myc pathways. also induces irregular estrus fibrosis aging mice, decreases serum AMH levels, increases FSH levels. Systemically, elevated IgG levels contribute osteoporosis cognitive decline, both linked dysfunction POF OEM. These findings elucidate mechanisms driving highlight broader effects. This study emphasizes importance monitoring health ectopic tissue safeguard reserves mitigate risks such as decline.

Language: Английский

Citations

0
The 3C (Cell Culture, Computer Simulation, Clinical Trial) Solution for Optimizing the 3R (Replace, Reduction, Refine) Framework during Preclinical Research Involving Laboratory Animals DOI
Sounak Roy,

Mubeena Parveen,

Asis Bala

et al.

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
Emerging targets for advancing endometrial therapeutics DOI Creative Commons
Signe Altmäe,

Aida Rodríguez-Santisteban

Reproductive BioMedicine Online, Journal Year: 2025, Volume and Issue: 50(4), P. 104785 - 104785

Published: April 1, 2025

The endometrium plays a crucial role in female health. Globally, millions of women are affected by endometrial/uterine disorders, yet the and its gynaecological pathologies have been understudied. Gaining insight into detailed endometrial architecture, gene expression, spatial temporal cellular interactions, microenvironment is essential for understanding physiology pathophysiology this dynamic tissue. current paper highlights latest targets advancing research that include single-cell RNA sequencing, transcriptomics, microbiome, organoid models, analysis menstrual blood less-studied ageing. authors hope summary will provide more novel methods, highlight therapeutics inspire readers to generate fresh ideas future avenues.

Language: Английский

Citations

0
PYK2 promotes cell proliferation and epithelial-mesenchymal transition in endometriosis by phosphorylating Snail1 DOI Creative Commons
Lu Liu,

Lan Liu,

Congbo Yue

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: April 27, 2025

Abstract Background Endometriosis can lead to decreased endometrial receptivity, reduced rates of implantation, and diminished ovarian reserve. Currently, more than 50% infertile women are found suffer from endometriosis. However the etiology pathogenesis endometriosis still poorly understood. Epithelial-mesenchymal transition (EMT) has been confirmed be involved in PYK2 is a non-receptor tyrosine kinase that affects cell proliferation, survival, migration by regulating intracellular signaling pathways. plays regulatory role EMT process affecting expression genes associated with through influence transcription factors. Snail1 (Snail1) key highly expressed tissues. On other hand, invasive metastatic ability cells mainly process. However, upstream mechanisms regulate protein stability not clear. Methods We identified kinase, proline-rich 2 (PYK2 or PTK2B), examined The relevant plasmids were constructed. This study enrolled 20 patients laparoscopically meeting ASRM diagnostic criteria, collecting ectopic lesions (14 endometriotic cysts 6 deep infiltrating nodules) along matched eutopic tissues (15 proliferative phase, 5 secretory phase) as controls. All tissue specimens underwent immunohistochemical analysis. Human stromal (HESC) isolated normal endometrium 3 control for vitro meconium induction. Ectopic (EESC) obtained lesions. Protein extracts both subjected Western blot co-immunoprecipitation (Co-IP) interaction validation. Functional assays (proliferation/migration/invasion) performed using EESC 11Z lines triplicate biological replicates. Co-IP experiments verify between Snail1, well determine specific location this interaction. Additionally, we effect on whether VS-6063 inhibits functions cells. models established five-week-old female C57BL/6 mice, randomly allocated into experimental ( n = 10) groups. Statistical analyses conducted GraphPad Prism 7.0, employing parametric tests normally distributed data non-parametric methods otherwise, Benjamini-Hochberg correction multiple comparisons. Results It acts new binding partner enhances increasing phosphorylation Snail1. promotes migration, invasion while inhibiting decidualization. demonstrated inhibited vitro, growth vivo. Conclusions novel occurrence development up-regulating which could promising therapeutic target

Language: Английский

Citations

0
Single-cell insights into epithelial morphogenesis in the neonatal mouse uterus DOI Creative Commons
Thomas E. Spencer, Makenzie T Lowke, Kimberly M. Davenport

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(49)

Published: Nov. 29, 2023

The uterus is vital for successful reproduction in mammals, and two different types of epithelia (luminal glandular) are essential embryo implantation pregnancy establishment. However, the cellular molecular factors pathways governing postnatal epithelium maturation, determination, differentiation developing yet to be elucidated. Here, neonatal mouse was isolated subjected single-cell transcriptome (scRNA-seq) analysis. Both undifferentiated determined luminal were heterogeneous contained several cell clusters based on transcription profiles. Substantial gene expression differences evident as matured differentiated between days 1 15. Two new glandular epithelium-expressed genes ( Gas6 Cited4 ) identified validated by situ hybridization. Trajectory analyses provided a framework understanding lineage bifurcation, differentiation. A candidate set regulatory networks that potentially direct specification morphogenesis. This atlas provides foundation important discover intrinsic mechanisms directing uterine morphogenesis during critical window development.

Language: Английский

Citations

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