Bioengineering approaches for the endometrial research and application

Materials Today Bio, Год журнала: 2024, Номер 26, С. 101045 - 101045

Опубликована: Апрель 3, 2024

The endometrium undergoes a series of precise monthly changes under the regulation dynamic levels ovarian hormones that are characterized by repeated shedding and subsequent regeneration without scarring. This provides potential for wound healing during endometrial injuries. Bioengineering materials highlight faithful replication constitutive cells extracellular matrix simulates physical biomechanical properties to larger extent. Significant progress has been made in this field, functional tissue bioengineering allows an in-depth investigation regulatory factors myometrial defects vitro highly therapeutic methods alleviate obstetric gynecological complications. However, much remains be learned about latest application technologies human endometrium. Here, we summarize existing developments biomaterials models improving female reproductive potential.

Язык: Английский

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Construction organoid model of ovarian endometriosis and the function of estrogen and progesterone in the model

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 24, 2025

Abstract Endometriosis is a refractory estrogen-dependent gynecological disease in which ovarian endometriosis(OE) the most common, and main cell components are endometrial epithelial cells stromal cells. However, constructing ectopic models basic studies still challenging. In this study, we explored feasibility influencing factors of validating eutopic organoid OE as in-vitro models. Eutopic tissues patients were selected to establish organoids. Morphologically, organoids showed three-dimensional glandular structure with vacuoles or cystic irregularities, histological features endometriosis well preserved. Immunofluorescence positive expression markers estrogen/progesterone receptors. Genetic identification revealed 100% match between tissue, indicating common origin. The effects estrogen progesterone on proliferation secretion differed change concentration. successful construction provides new vitro model for drug intervention mechanism study endometriosis.

Язык: Английский

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Revolutionizing the female reproductive system research using microfluidic chip platform

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Дек. 19, 2023

Abstract Comprehensively understanding the female reproductive system is crucial for safeguarding fertility and preventing diseases concerning women's health. With capacity to simulate intricate physio- patho-conditions, provide diagnostic platforms, microfluidic chips have fundamentally transformed knowledge management of health, which will ultimately promote development more effective assisted technologies, treatments, drug screening approaches. This review elucidates diverse systems in mimicking ovary, fallopian tube, uterus, placenta cervix, we delve into culture follicles oocytes, gametes’ manipulation, cryopreservation, permeability especially. We investigate role microfluidics endometriosis hysteromyoma, explore their applications ovarian cancer, endometrial cancer cervical cancer. At last, current status technology integrated devices are introduced briefly. Through delineating multifarious advantages challenges technology, chart a definitive course future research woman health field. As continues evolve advance, it holds great promise revolutionizing diagnosis treatment issues, thus propelling us where can optimize overall wellbeing women everywhere. Graphical

Язык: Английский

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Advances in Nanomedicine and Biomaterials for Endometrial Regeneration: A Comprehensive Review

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 8285 - 8308

Опубликована: Авг. 1, 2024

The endometrium is an extremely important component of the uterus and crucial for individual health human reproduction.However, traditional methods still struggle to ideally repair structure function damaged restore fertility.Therefore, seeking developing innovative technologies materials has potential regenerate or diseased endometrium.The emergence functionalization various nanomedicine biomaterials, as well proposal development regenerative medicine tissue engineering techniques, have brought great hope solving these problems.In this review, we will summarize nanomedicine, that contribute endometrial regeneration, including nanoscale exosomes, nanomaterials, stem cell-based materials, naturally sourced chemically synthesized approaches functionalizing application revolutionary new such organoids, organ-on-chips, artificial intelligence, etc.The diverse design modification biomaterials endow them with functionalities, microstructure nanostructure, mechanical properties, biological functions, cellular microenvironment regulation.It provide options regeneration endometrium, bring reconstruction recovery patients' reproductive abilities.

Язык: Английский

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Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube

Science Advances, Год журнала: 2024, Номер 10(39)

Опубликована: Сен. 27, 2024

The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the tube that molecularly, functionally, architecturally resembles organ. Global label-free proteomics, innovative assays capturing physiological functions (i.e., oocyte transport), whole-organ single-cell resolution mapping used validate these assembloids through multifaceted platform with direct comparisons tissue. These techniques converge at unique combination parameters highest similarity reference tube. This work establishes (i) an optimized human for vitro studies pathophysiology (ii) iterative customized models organs microanatomically accurate by combining tunable tissue methods.

Язык: Английский

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Hydrogel Strategies for Female Reproduction Dysfunction

ACS Nano, Год журнала: 2024, Номер 18(44), С. 30132 - 30152

Опубликована: Окт. 22, 2024

Infertility is an important issue for human reproductive health, with over half of all cases infertility associated female factors. Dysfunction the complex system may cause infertility. In clinical practice, often treated oral medications and/or surgical procedures, and ultimately assisted technologies. Owing to their excellent biocompatibility, low immunogenicity, adjustable mechanical properties, hydrogels are emerging as valuable tools in reconstruction organ function, supplemented by tissue engineering techniques increase structure functionality. Hydrogel-based strategies targeting pathological mechanisms provide alternatives treatment ovarian, endometrium/uterine, fallopian tube dysfunction. this review, we a general introduction basic physiology pathology system, limitations current treatments, lack translation from animal models physiology. We further overview future potential applications dysfunction, highlighting great prospects hydrogel-based field translational medicine, along significant challenges be overcome.

Язык: Английский

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Current advances in understanding endometrial epithelial cell biology and therapeutic applications for intrauterine adhesion

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 25, 2024

The human endometrium is a highly regenerative tissue capable of undergoing scarless repair during the menstruation and postpartum phases. This process mediated by endometrial adult stem/progenitor cells. During healing injuries, swift reepithelization results in rapid covering wound surface facilitates subsequent restoration. involvement endogenous epithelial stem cells, stromal bone marrow-derived cells regeneration epithelium has been subject prolonged debate. Increasing evidence suggests that mainly relies on rather than Currently, no consensus established identity compartment. Several markers, including stage-specific embryonic antigen-1 (SSEA-1), sex-determining region Y-box 9 (SOX9), neural-cadherin (N-cadherin), leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5), CD44, axis inhibition protein 2 (Axin2), aldehyde dehydrogenase 1A1 (ALDH1A1), have suggested as potential candidate markers for identification contributes to our understanding offers new therapeutic insights into diseases characterized defects endometrium, such intrauterine adhesion. review explores different perspectives origins mouse It summarizes locations, hierarchies both endometrium. also discusses cell-based treatments adhesion, hoping inspire further research clinical application

Язык: Английский

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Lactobacillus-Loaded Easily Injectable Hydrogel Promotes Endometrial Repair via Long-Term Retention and Microenvironment Modulation

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

Regeneration of the injured endometrium, particularly functional layer, is crucial for prevention uterine infertility. At present, clinical treatment using sodium hyaluronate hydrogel injection limited by its relatively low fluidity, short-term retention, and insufficient bioactive ingredients, so it necessary to develop an advanced healing-promoting hydrogel. The modulation microenvironment Lactobacillus presents a component that can facilitate regeneration layer. Our study introduces multifunctional Lactobacillus-loaded poly(N-isopropylacrylamide)-grafted bacterial cellulose (BC-g-PN@L) designed with superior injectability in situ stability. 25 °C (room temperature), uniform distribution achieved pressure only 7.90 kPa. 37 (body BC-g-PN@L forms robust three-dimensional nanonetwork, providing space substance exchange channels maintain viability bioactivity. Enhanced hydrophobic isopropyl groups poly(N-isopropylacrylamide) side chains rigid substrates, exhibits prolonged retention properties cavity, persisting over 21 days. These attributes endow versatile pro-healing capacity rat model endometrial injury. promotes development injectable hydrogels both histological repair endometrium.

Язык: Английский

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Enhanced myofibroblast differentiation of eMSCs in intrauterine adhesions

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 4, 2025

Intrauterine adhesions (IUA) is one of the most common gynecological diseases and main causes uterine infertility. Among proposed hypotheses on IUA development, reduced endometrial regeneration resulting from loss functional stem cells has been as key factor affecting prognosis. However, underlying mechanisms mostly remain unclear. Because eMSCs (endometrial mesenchymal stem/stromal cells) play a critical role in both supporting gland development also preparing environment for embryo implantation through decidualization, characteristics functions were compared between derived non-IUA patients, to uncover important roles mechanisms. Endometrium biopsies collected patients controls. The fibrosis features eMSC distributions investigated with IHC (immunohistochemistry). Then isolated their analyzed vitro. Our results indicate that scar tissues are characterized hyper-activation pro-fibrotic fibroblast myo-differentiation, along number eMSCs. controls show similar perspectives cell morphology, proliferation, colony formation, exosome secretion, positive ratio markers conventional MSC markers, tri-differentiation efficiency, ability suppressing lymphocyte aging, promoting vascular tube formation. have levels decidualization higher migration, invasion, myofibroblast differentiation. Further investigations TGF-β pathway, which major inducer differentiation, up-regulated responsible enhanced differentiation potential IUA. In conclusion, we demonstrated here biopsy myofibroblast. tissues, capability increased.

Язык: Английский

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Multiresponsive Microcapsules for Prevention of Intrauterine Adhesion

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Intrauterine adhesion (IUA) has a significant negative impact on women's reproductive health. One of the development trends biomaterials for prevention IUA is improving stability and multifaceted functions. Here, we propose multiresponsive microcapsule (A/G-Fe3O4-Se) with an alginate (ALG) gelatin methacryloyl (GelMA) dual-network hydrogel shell loaded magnetic nanoparticles (Fe3O4-Se) ultrasound-responsive decafluoropentane core using microfluidic technique. The microcapsules inherited biofriendly advantages ALG GelMA. encapsulated magneto-responsive Fe3O4-Se made flexibly change distribution to adapt irregular shape uterus better exert therapeutic effect. Besides, A/G-Fe3O4-Se demonstrated antioxidant, antibacterial, pro-healing properties in vitro. Moreover, rat models, also observed reduction oxidative stress, endometrial regeneration, improved receptivity pregnancy rates after treatment microcapsules. Consequently, can be used as promising strategy damaged endometrium well IUA.

Язык: Английский

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