Frontiers in Cellular and Infection Microbiology,
Journal Year:
2022,
Volume and Issue:
11
Published: Jan. 5, 2022
Background
Despite
the
obvious
advantages
of
metagenomic
next-generation
sequencing
(mNGS)
in
etiological
diagnosis
various
infectious
diseases,
there
are
few
reports
on
suspected
thoracic
and
abdominal
infections
patients
with
end-stage
liver
disease
(ESLD).
Methods
Seventy-three
ESLD
were
enrolled
from
January
2019
to
May
2021
due
complicated
poor
response
empirical
anti-infective
treatment.
Pleural
effusion
ascites
samples
these
collected
for
mNGS
detection
conventional
pathogen
culture.
The
application
value
was
finally
evaluated.
Results
A
total
96
pathogens
detected
using
method,
including
47
bacteria,
32
viruses,
14
fungi,
2
Mycobacterium
tuberculosis
,
1
parasite.
positive
rate
reached
42.5%,
which
significantly
higher
than
that
culture
method
(21.9%)
(p
=
0.008).
Considering
neutrophil
counts,
overall
bacteria
both
methods
Polymorphonuclear
Neutrophils
(PMN)
≥250/mm
3
group
64.3%
PMN
<250/mm
23.7%.
Compared
final
clinical
diagnosis,
agreement
78.6%
(11/14)
44.4%
(8/18),
respectively.
In
addition,
66.7%
(4/6,
respectively)
fungal
detection.
Interestingly,
11
detection,
5
had
alcoholic
disease,
accounting
45.5%
all
disease.
We
also
strains
viruses
mNGS,
mainly
cytomegalovirus
(62.5%).
Conclusions
is
a
useful
supplement
methods,
performs
rate,
sensitivity,
broader
spectrum,
especially
rare
those
difficult
For
patients,
has
great
prospects
early
infections.
cutoff
values
bacterial
infection
(PMN
)
cavities
may
need
be
redefined.
Journal of Hepatology,
Journal Year:
2021,
Volume and Issue:
76(2), P. 332 - 342
Published: Sept. 24, 2021
Rifaximin-α
is
efficacious
for
the
prevention
of
recurrent
hepatic
encephalopathy
(HE),
but
its
mechanism
action
remains
unclear.
We
postulated
that
rifaximin-α
reduces
gut
microbiota-derived
endotoxemia
and
systemic
inflammation,
a
known
driver
HE.
Clinical Microbiology Reviews,
Journal Year:
2024,
Volume and Issue:
37(2)
Published: May 8, 2024
SUMMARYGiven
the
importance
of
gut
microbial
homeostasis
in
maintaining
health,
there
has
been
considerable
interest
developing
innovative
therapeutic
strategies
for
restoring
microbiota.
One
such
approach,
fecal
microbiota
transplantation
(FMT),
is
main
"whole
microbiome
replacement"
strategy
and
integrated
into
clinical
practice
guidelines
treating
recurrent
The Lancet. Gastroenterology & hepatology,
Journal Year:
2023,
Volume and Issue:
8(6), P. 523 - 532
Published: March 6, 2023
Alcohol
is
the
leading
cause
of
liver-related
mortality
worldwide.
The
gut-liver
axis
considered
a
key
driver
in
alcohol-related
liver
disease.
Rifaximin-α
improves
gut-barrier
function
and
reduces
systemic
inflammation
patients
with
cirrhosis.
We
aimed
to
compare
efficacy
safety
rifaximin-α
placebo
Journal of Hepatology,
Journal Year:
2024,
Volume and Issue:
81(2), P. 345 - 359
Published: March 28, 2024
The
rising
prevalence
of
liver
diseases
related
to
obesity
and
excessive
use
alcohol
is
fuelling
an
increasing
demand
for
accurate
biomarkers
aimed
at
community
screening,
diagnosis
steatohepatitis
significant
fibrosis,
monitoring,
prognostication
prediction
treatment
efficacy.
Breakthroughs
in
omics
methodologies
the
power
bioinformatics
have
created
excellent
opportunity
apply
technological
advances
clinical
needs,
instance
development
precision
personalised
medicine.
Via
technologies,
biological
processes
from
genes
circulating
protein,
as
well
microbiome
-
including
bacteria,
viruses
fungi,
can
be
investigated
on
axis.
However,
there
are
important
barriers
omics-based
biomarker
discovery
validation,
semi-quantitative
measurements
untargeted
platforms,
which
may
exhibit
high
analytical,
inter-
intra-individual
variance.
Standardising
methods
need
validate
them
across
diverse
populations
presents
a
challenge,
partly
due
disease
complexity
dynamic
nature
expression
different
stages.
Lack
validity
causes
lost
opportunities
when
studies
fail
provide
knowledge
needed
regulatory
approvals,
all
contributes
delayed
translation
these
discoveries
into
practice.
While
no
matured
implementation,
extent
data
generated
has
enabled
hypothesis-free
plethora
candidate
that
warrant
further
validation.
To
explore
many
hepatologists
detailed
commonalities
differences
between
various
layers,
both
advantages
approaches.
